Disposable analytical device

• US 4,865,813 A
• Filed: 07/07/1986
• Issued: 09/12/1989
• Est. Priority Date: 07/07/1986
• Status: Expired due to Fees

• Current Assignee: Luis P. Leon
• Original Assignee: Luis P. Leon
• Inventors: Leon, Luis P.
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): Kummert, Lynn M.
• Application Number:
  US06/882,167
• Time in Patent Office: 1,163 Days
• Field of Search: 422/44, 422/56, 422/58, 422/59, 422/61, 422/68, 422/101, 422/102, 436/165, 436/177, 436/178, 436/180, 436/808, 436/810, 210/198.1, 210/500.21, 210/511, 210/321.84, 210/345
• US Class Current: 422/82.05
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: G01N 2021/0325 Cells for testing reactions, e.g. containing reagents
• CPC Class Codes: G01N 2021/0385 Diffusing membrane; Semipermeable membrane
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: Y10S 436/808 Automated or kit
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

The disposable analytical device includes an outer shell portion having an interior which is divided into reagent chambers and a test sample chamber. The reagent chambers are non-communicable with each other. However the test sample chamber is communicable with each of the reagent chambers through a semi-permeable membrane. The device is formed of an optic quality material to permit analysis of the test results in a spectrophotometer.

Claims

1. An article of manufacture in the form of a disposable analytical device for testing the properties of a test sample comprising:

(a) a container having opposite base portions and a non-permeable imperforate outer peripheral portion extending between said opposite base portions to define an enclosed interior space,

(b) a semi-permeable membrane within said outer peripheral portion extending from one said base portion to the other said base portion for dividing said interior space into separate chambers having corresponding opposite closed base ends corresponding to said opposite base portions,

(c) a test sample to be analyzed being chargeable in one of said chambers through one of said base portions, the opposite base portions being imperforate,

(d) the other of said chambers being sealed at said opposite base ends by said opposite base portions and including nonpermeable baffle means to subdivide the other said chamber into at least two noncommunicable reagent chambers each said reagent chamber having a predetermined analytical reagent contained therein, said non-permeable baffle means extending away from said semi-permeable membrane to said outer peripheral portion to permit common communication between said one of said chambers and the respective said at least two reagent chambers,

(e) said semi-permeable membrane and said analytical reagents being selected such that communication occurs from said one of said chambers through said semi-permeable membrane to each of said at least two reagent chambers,

(f) said container having no inlet nor outlet flow means such that any fluid disposed within said one of said chambers and said at least two reagent chambers remains inside said container, and

(g) said base portions being light transmitting for permitting light to pass through said at least two reagent chambers in predetermined fashion for light measurement purposes.

2. The device as claimed in claim 1 wherein said semi-permeable membrane joins the opposite base portions in leak-tight arrangement.

3. The device as claimed in claim 1 wherein one of said base portions comprises a cover member through which said one chamber is charged with said test sample.

4. The device as claimed in claim 1 wherein said one of said base portions comprises a cover member, said outer peripheral portion and said other base portion together constituting a component of said device that is attachable to said shell portion to form said one base portion of said device.

5. The device as claimed in claim 1 wherein said semi-permeable membrane is supported by a perforated support member.

6. The device as claimed in claim 1 wherein said container is of a first predetermined size and shape and has a first closed periphery and a first cross-section, said semi-permeable membrane being of a second predetermined size and shape and having a second closed periphery and a second cross-section, said semi-permeable membrane being disposed in said container such that said second closed periphery is spaced radially inwardly from said first closed periphery.

7. The device as claimed in claim 6 including a space within said second closed periphery and wherein the space within said second closed periphery defines one of said chambers and the space between said first and second closed peripheries defines the other of said chambers.

8. The device as claimed in claim 6 wherein said container and said semi-permeable membrane are generally circular in cross section.

9. The device as claimed in claim 1 wherein said baffle means comprises a baffle member having opposite end portions joined to the respective opposite base portions of said container.

10. The device as claimed in claim 9 wherein said baffle means comprises a predetermined plurality of said baffle members spaced from each other to define a corresponding predetermined plurality of said reagent chambers.

11. The device as claimed in claim 10 wherein said container and said semi-permeable membrane are generally circular in cross section and said baffle members extend radially away from said semi-permeable membrane to said non-permeable outer peripheral portion.

12. The device of claim 11 wherein one of said reagent chambers is a reference chamber for electrical testing and includes electrical terminals at opposite base portions of said container, a non-permeable wall being provided between said reference chamber and said test sample chamber.

13. The device as claimed in claim 11 wherein said non-permeable outer peripheral portion and said baffle member are integrally joined.

14. The device as claimed in claim 10 including four baffle members in said other of said chambers to divide said other of said chambers into four non-communicable reagent chambers.

15. The device as claimed in claim 10 including eight baffle members in said other of said chambers to divide said other of said chambers into eight non-communicable reagent chambers.

16. The device as claimed in claim 10 including five baffle members in said other of said chambers to divide said other of said chambers into six non-communicable reagent chambers.

17. The device as claimed in claim 1 wherein said container is of a first predetermined size and shape and has a first closed periphery, said semi-permeable membrane being of a second predetermined size and shape and extending across the first closed periphery of said container.

18. The device as claimed in claim 17 wherein said semi-permeable membrane extends across said first closed periphery to divide the enclosed interior space into a space on one side of said semi-permeable membrane that defines said one chamber and a space on the other side of said semi-permeable membrane defines said other chamber.

19. The device as claimed in claim 17 wherein non-permeable baffle means are provided in said other chamber to subdivide the other of said chambers into at least-two reagent chambers.

20. An article of manufacture in the form of a disposable analytical device for testing the properties of a test sample comprising,

(a) a container having an imperforate non-permeable outer peripheral wall, a base and a cover member to define an enclosed interior space,

(b) a semi-permeable membrane extending from said base to said cover member to divide said interior space into two separate chambers having corresponding opposite closed base ends corresponding to said base and said cover member, respectively,

(c) a test sample to be analyzed being disposable in one of said chambers,

(d) the other of said chambers being sealed at said opposite ends by said base and said cover member, respectively and including nonpermeable baffle means to subdivide the other said chamber into at least two noncommunicable reagent chambers, each said reagent chamber having a predetermined analytical reagent container therein, said nonpermeable baffle means extending away from said semipermeable membrane to said outer peripheral wall to permit common communication between said one of said chambers and the respective said at least two reagent chambers,

(e) said semi-permeable membrane and said analytical reagents being selected such that communication occurs from said one of said chambers through said semi-permeable membrane to each of said at least two reagent chambers, and

(f) said base and said cover member being light transmitting for permitting light to pass through said at least two reagent chambers in predetermined fashion for light measurement purposes.

21. An article of manufacture in the form of a disposable analytical device for testing the properties of a test sample comprising,

(a) a container having opposite base portions and a non-permeable imperforate outer peripheral portion extending between said opposite base portions to define an enclosed interior space,

(b) a pair of spaced apart semi-permeable membranes within said outer peripheral portion extending from one said base portion to the other said base portion and between opposite sides of said outer peripheral portion for dividing said interior space into three separate chambers having corresponding opposite closed base ends corresponding to said opposite base portions,

(c) a test sample to be analyzed being chargeable in one of said three chambers defined in part by said pair of semi-permeable membranes, through one of said base portions, the opposite base portion being imperforate,

(d) the other two of said three chambers being reagent chambers sealed at said opposite base ends by said opposite base portions and each of said reagent chambers having a predetermined analytical reagent contained therein,

(e) said semi-permeable membranes and said analytical reagents selected such that communication occurs from said one chamber through the respective said semi-permeable membranes to the respective said two reagent chambers,

(f) said container having no inlet nor outlet flow means such that any fluid disposed within said one chamber and said two reagent chambers remains inside said container, and

(g) said base portions being light transmitting for permitting light to pass through said two reagent chambers in predetermined fashion for light measurement purposes.

23. The device as claimed in claim 21 wherein said semi-permeable membrane joins the opposite base portions in leak-tight arrangement.

24. The device as claimed in claim 21 wherein one of said base portions comprises a cover member through which said one chamber is charged with said test sample.

25. The device as claimed in claim 21 wherein said one of said base portions comprises a cover member, said outer peripheral portion and said other base portion together constituting a shell portion, said cover member being a positionable component of said device that is attachable to said shell portion to form said one base portion of said device.

26. The device as claimed in claim 21 wherein said semi-permeable membranes are supported by a perforated support member.

22. An article of manufacture in the form of a disposable analytical device for testing the properties of a test sample comprising,

(a) a container having an imperforate non-permeable outer peripheral wall, a base and a cover member to define an enclosed interior space,

(b) a pair of spaced apart semi-permeable membranes extending from said base to said cover member and between opposite sides of said outer peripheral wall to divide said interior space into three separate chambers wherein two of said chambers are spaced from each other by the third said chamber, said third chamber being defined in part by the spaced-apart semi-permeable membranes, said two of said chambers are non-communicable with each other but are each communicable with said third chamber via said semi-permeable membranes, said three chambers having corresponding opposite closed base ends corresponding to said base and said cover member, respectively,

(c) a test sample to be analyzed being disposable in said third chamber,

(d) said two of said chambers being reagent chambers sealed at said opposite base ends by said base and said cover member, respectively, and each of said reagent chambers having a predetermined analytical reagent contained therein,

(e) said semi-permeable membrane and said analytical reagents being selected such that communication occurs from said third chamber through the respective said semi-permeable membranes to the respective said two reagent chambers, and

(f) said base and said cover member being light transmitting for permitting light to pass through said two reagent chambers in predetermined fashion for light measurement purposes.