×

Method for cryopreparing biological tissue

  • US 4,865,871 A
  • Filed: 07/11/1988
  • Issued: 09/12/1989
  • Est. Priority Date: 08/23/1983
  • Status: Expired due to Term
First Claim
Patent Images

1. A method for cryopreparing a biological tissue sample comprising:

  • treating a biological tissue sample with a cryoprotectant;

    vitrifying the tissue sample at a rate and under conditions so as to preclude the formation of significant resolvable ice crystals;

    equilibrating said vitrified tissue sample at temperature and pressure conditions below the glass transition range of said sampleadding energy to said equilibrated tissue sample at a rate sufficient to cause vitrified water in said sample to change physical state directly to the vapor phase without substantially disturbing said equilibrium or completing the induced transition; and

    removing water from said tissue sample by molecular distillation by the addition of energy until substantially no vaporized water can be detected in the atmosphere surrounding said tissue sample.

View all claims
  • 3 Assignments
Timeline View
Assignment View
    ×
    ×