Temperature control apparatus

• US 4,865,986 A
• Filed: 10/06/1988
• Issued: 09/12/1989
• Est. Priority Date: 10/06/1988
• Status: Expired due to Fees

• Current Assignee: COY Pty., Limited
• Original Assignee: COY Pty., Limited
• Inventors: Waycaster, Roy A., Coy, Richard A.
• Primary Examiner(s): Jones, Larry
• Application Number:
  US07/254,255
• Time in Patent Office: 341 Days
• Field of Search: 435/290, 435/300, 435/316, 435/286, 435/289, 435/288, 435/296, 422/67, 422/109, 422/173, 422/198, 422/202, 422/285, 422/290, 422/307
• US Class Current: 435/285.1
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: F25B 21/02 using Peltier effect; using Nernst-Ettinghausen effect

Abstract

Apparatus is disclosed for controlling the heating and cooling of a plurality of upright containers containing a mixture used for performing gene amplification. The apparatus includes a support rack comprising aluminum blocks which is partially submerged in a thermally conductive fluid such that at least the lower portions of the containers are submerged in the fluid with the upper portions engaging the aluminum blocks for efficient heat transfer. Heaters are disposed within the aluminum block for heating the block and a plurality of thermoelectric cooling cells are used to cool the block. A programmable microprocessor is used for controlling the heating and cooling cycles, thereby allowing repetitive heating and cooling of the mixture to produce the copies of the genetic material sought to be copied. A cam separates the support rack from the cooling cells during the heating portion of the process.

Claims

1. Apparatus for selectively heating and cooling a plurality of upright containers and their contents comprising:

a metal heating block with vertical cavities therethrough for supporting said upright containers;

a container for holding a quantity of thermally conductive fluid and for reception of said heating block within said fluid container with a portion of said upright containers in contact with the fluid;

means for heating the metal heating block and the fluid, said heating means being disposed within said heating block between said vertical cavities;

a metal cooling block below the fluid container and in vertical surface to surface engagement with said fluid container;

thermoelectric cooling means beneath said cooling block and engaging said cooling block for cooling said heating block and fluid;

temperature monitoring means within said heating block; and

means selectively operative to provide alternatively for vertical separation of the fluid container and cooling block and vertical surface to surface engagement to enable heating of the contents of the upright containers rapidly and cooling of the contents of the upright containers rapidly while maintaining precise temperature conditions for precise periods of time.

2. The apparatus of claim 1 wherein said selectively operable means includes an electric motor.

3. The apparatus of claim 2 wherein said selectively operable means includes cam means disposed between said fluid container and said cooling block, said cam means rotatable from an engagement position wherein said fluid container and cooling block are in surface to surface engagement to a separation position wherein said cam means separates said fluid container from said cooling block, said cam means being rotatable by said electric motor.

4. The apparatus of claim 3 wherein said cam means includes an elongated flat plate having a greater width than thickness, said plate extending between the fluid container and cooling block and positioned within a longitudinal groove in the top of said cooling block and bottom of said fluid container.

5. The apparatus of claim 2 wherein said selectively operable means includes floating mount means for attaching said electric motor and cam means.

6. The apparatus of claim 1 wherein said heating means includes an electrical resistance heater.

7. The apparatus of claim 1 wherein said thermoelectric cooling means includes peltier cells.

8. The apparatus of claim 1 further comprising control means for selectively activating said heating and cooling means.

9. An apparatus for selectively heating and cooling a plurality of upright containers and the contents thereof, comprising:

a support rack having a plurality of metal blocks in a side-by-side relationship, each of said blocks having a plurality of vertical apertures therethrough for supporting said upright containers;

a container for holding a quantity of thermally conductive fluid for submerging a portion of said upright containers therein by placing said rack in said fluid container;

electric resistance heaters disposed between said metal blocks and engaging said blocks for heating said support rack and fluid and thereby heating said upright container;

a cooling block beneath said fluid container, said cooling block engageble with the bottom of said container;

a plurality of thermoelectric cooling cells beneath said cooling block and engaging said cooling block for cooling said cooling block, fluid container, support rack and fluid, thereby cooling said upright containers;

a thermocouple disposed within said support rack for monitoring the temperature of said rack;

control means for activating the heaters and cooling cells for alternating heating and cooling said upright containers; and

means operatively associated with said fluid container and cooling block for selectively disengaging and engaging said fluid container and said cooling block.

10. The apparatus of claim 9 wherein said disengaging and engaging means includes cam means disposed between said fluid container and said cooling block, said cam means rotatable from an engagement position wherein said fluid container and cooling block are in surface to surface engagement to a separation position wherein said cam means separates said fluid container from said cooling block.