Protein adsorbent including a colored compound containing a quaternary ammonium especially amidinium or guanidimium cationic group and a cellulose reactive group
First Claim
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1. A protein adsorbent suitable for use in the chromatographic separation of complex biological molecules which comprises a coloured compound having the formula:
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space="preserve" listing-type="equation">A--Y--Bor,
space="preserve" listing-type="equation">Y'"'"'--Bwherein A is a cationic group selected from (i) an acyclic or cyclic fully substituted ammonium group of the formulae;
##STR4## wherein R1 to R4 are alkyl, alkenyl or aryl groups, B represents an aromatic radical and D represents an alicyclic radial in which at least one of R1 or R4, B and D contains a free valency, and (ii) an amidinium or guandiinium group of the formula;
##STR5## wherein R6 to R9 are H or alkl, alkenyl or aryl groups and n is 0 or 1;
Y is a chromophore;
Y'"'"' is a cationic chromophore; and
B is a mono- or poly-halogenoheterocycle selected from dichloropyrimidinyl, difluoropyrimidinyl, trichloropyrimidinyl, 5-chlorodifluoropyrimidinyl, 5-cyanodichloropyrimidinyl, dichlorotriazinyl and monochlorotriazinyl bound to an inert substrate.
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Abstract
A colored compound containing a cationic group and a cellulose reactive group, especially a compound of the formula:
A--Y--B I
Y'"'"'--B II
wherein
A is the cationic group;
Y is a chromophore;
Y'"'"' is a cationic chromophore
and
B is the reactive group, which is suitable for the preparation of a protein adsorbent or precipitant for use in the separation of mixtures of proteins.
46 Citations
4 Claims
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1. A protein adsorbent suitable for use in the chromatographic separation of complex biological molecules which comprises a coloured compound having the formula:
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space="preserve" listing-type="equation">A--Y--Bor,
space="preserve" listing-type="equation">Y'"'"'--Bwherein A is a cationic group selected from (i) an acyclic or cyclic fully substituted ammonium group of the formulae;
##STR4## wherein R1 to R4 are alkyl, alkenyl or aryl groups, B represents an aromatic radical and D represents an alicyclic radial in which at least one of R1 or R4, B and D contains a free valency, and (ii) an amidinium or guandiinium group of the formula;
##STR5## wherein R6 to R9 are H or alkl, alkenyl or aryl groups and n is 0 or 1;
Y is a chromophore;
Y'"'"' is a cationic chromophore; and
B is a mono- or poly-halogenoheterocycle selected from dichloropyrimidinyl, difluoropyrimidinyl, trichloropyrimidinyl, 5-chlorodifluoropyrimidinyl, 5-cyanodichloropyrimidinyl, dichlorotriazinyl and monochlorotriazinyl bound to an inert substrate. - View Dependent Claims (2, 3, 4)
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Specification