Method for measuring a concentration of saccharides having a low molecular weight and sensor for measuring the same

• US 4,865,995 A
• Filed: 02/04/1986
• Issued: 09/12/1989
• Est. Priority Date: 02/04/1985
• Status: Expired due to Fees

• Current Assignee: Kanegafuchi Kagaku Kogyo Kabushiki Kaisha
• Original Assignee: Kanegafuchi Kagaku Kogyo Kabushiki Kaisha
• Inventors: Dairaku, Kazuo
• Primary Examiner(s): Nucker, Christine M.
• Assistant Examiner(s): Johnston, Jill
• Application Number:
  US06/826,038
• Time in Patent Office: 1,316 Days
• Field of Search: 436/501, 436/514, 436/529, 436/94, 436/95, 436/178, 436/536, 436/178, 435/288, 435/299, 435/311, 435/7, 422/68, 422/69
• US Class Current: 436/94
• CPC Class Codes: B01D 15/36 involving ionic interaction
• CPC Class Codes: C12M 41/32 of substances in solution
• CPC Class Codes: G01N 21/35 using infrared light G01N21/39 takes precedence
• CPC Class Codes: G01N 21/3577 for analysing liquids, e.g. polluted water
• CPC Class Codes: G01N 30/02 Column chromatography
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/144444 Glucose
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A method for measuring the concentration of low molecular weight saccharides in a liquid by placing the liquid in contact with one side of a membrane which allows saccharides to pass through, but not polysaccharides. A detecting liquid containing polysaccharides is placed in contact with the other side of the membrane. The detecting liquid is also in contact with a ligand which has a higher affinity for the saccharides than for the polysaccharides. The concentration of saccharides is calculated from the measured change in the concentration of polysaccharides in the detecting liquid. A sensor for measuring the concentration of low molecular weight saccharides based on the same method is also described.

Claims

1. A method for measuring the concentration of saccharides having a low molecular weights dissolved in an aqueous medium, said method comprising the steps of:

(a) bringing a liquid to be analyzed in contact with one side of a membrane provided inside a permeation member, and bringing a detecting liquid in contact with the other side of said membrane inside said permeation member, wherein the detecting liquid contains an aqueous solution of blue dextran, and said membrane allows said saccharides having a low molecular weights to pass through, but does not allow blue dextran to pass through;

(b) circulating said detecting liquid from the permeation member to come into contact with a ligand provided inside an adsorption-desorption member, wherein said ligand has an affinity for both the saccharides having a low molecular weight and blue dextran, said affinity for the saccharides having a low molecular weight being greater than said affinity for blue dextran;

(c) measuring the concentration of blue dextran in the detecting liquid by measuring the absorption of light of a selected wavelength by the detecting; and

(d) calculating the concentration of saccharides having a low molecular weight in the liquid to be analyzed from the change in the concentration of blue dextran in the detecting liquid, said change being due to the adsorption or desorption of said saccharides having a low molecular weight by the ligand and the corresponding desorption or adsorption of blue dextran by the ligand.

2. The method of claim 1, wherein the saccharides having a low molecular weight are those having a mean molecular weight of not more than 1×

10³.

3. The method of claim 1 or 2, wherein the polysaccharides have a mean molecular weight of not less than 5×

10³.

4. The method of claim 3, wherein the polysaccharides have a mean molecular weight in the range of from 1×

10⁴ to 5×

10⁶.

5. The method of claim 1, wherein the ligand is lectin.

6. The method of claim 1, wherein the membrane is an ultrafiltration membrane.

7. The method of claim 1, wherein the membrane is a dialyzer.

8. A circulation-type sensor for measuring the concentration of saccharides having a low molecular weight dissolved in an aqueous medium, said sensor comprising:

(a) a permeation member;

(b) a membrane provided inside said permeation member;

(c) a detecting liquid disposed inside said permeation member on one side of said membrane and containing an aqueous solution of blue dextran, wherein said membrane allows said saccharides having a low molecular weight to pass through, but does not allow blue dextran to pass through;

(d) means for holding a liquid to be analyzed in contact with the other side of said membrane inside said permeation member;

(e) an adsorption--desorption member comprising a ligand, said ligand having an affinity for both the saccharides having a low molecular weight and blue dextran, said affinity for the saccharides having a low molecular weights being greater than said affinity for blue dextran;

(f) means for continuously circulating and conducting the detecting liquid between said permeation member and said adsorption--desorption member; and

(g) means for measuring the concentration of blue dextran in the detecting liquid by measuring the absorption of light of a selected wavelength by the detecting liquid.

9. The sensor of claim 8, wherein the permeation member and the adsorption-desorption member are united in one piece.

10. The sensor of claim 8, wherein the detecting liquid further contains an aqueous solution of said saccharides having a low molecular weight.

11. The sensor of claim 8, wherein said adsorption-desorption member is a column.