Analyte detection by means of energy transfer

• US 4,868,103 A
• Filed: 02/19/1986
• Issued: 09/19/1989
• Est. Priority Date: 02/19/1986
• Status: Expired due to Term

• Current Assignee: Enzo Diagnostics, Inc.
• Original Assignee: Enzo Biochem Incorporated
• Inventors: Wetmur, James G., Abrams, Samuel B., Rabbani, Elazar, Stavrianopoulos, Jannis
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Jay, Jeremy M.
• Application Number:
  US06/831,250
• Time in Patent Office: 1,308 Days
• Field of Search: 436/501, 436/821, 436/800, 436/518, 436/536, 436/537, 436/528, 436/805, 935/78, 435/6, 435/803, 435/5
• US Class Current: 435/5
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: Y10S 435/803 Physical recovery methods, e.g. chromatography, grinding
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/821 involving complement factors or complement systems

Abstract

A method is disclosed to detect the presence of an analyte. The method involves forming a complex comprising the analyte and a binding entity. The binding entity comprises a first partner of an energy transfer system. The complex is then contacted with a reporting entity to form a unit. The reporting entity comprises a second partner of the energy transfer system. The first partner and the second partner are within Furster'"'"'s radius of each other in the formed unit. The unit is irradiated with energy which can only be absorbed by one of said partners, namely, the energy donor, which then emits fluorescent energy. Some of this energy is absorbed by the other of said partners, namely, the energy acceptor, which also emits fluorescent energy. However, the fluorescent energy of the energy acceptor is of longer wavelength and in addition may be of substantially greater duration than the fluorescent energy of the energy donor. The detection of fluorescence at the longer wavelength or after a given time interval verifies the presence of the analyte.

Claims

1. An assay for detecting the presence of an analyte comprising the steps of:

a. forming a complex comprising said analyte and binding entity comprising an analyte recognition segment and a first partner of a fluorescent energy transfer system, wherein said first partner is selected from the group consisting of an energy donor and an energy acceptor, wherein said energy donor is a fluorescent aromatic agent or a lanthanide metal and said energy acceptor is a fluorescent, aromatic agent or a lanthanide metal, with the proviso that when said energy donor is a fluorescent, aromatic agent, then said energy acceptor is a fluorescent, aromatic agent or a lanthanide metal and when said energy donor is a lanthanide metal, then said energy acceptor is a fluorescent, aromatic agent;

b. contacting said complex with a reporting entity composition comprising a second partner of said fluorescent energy transfer system and at least one component for rendering said analyte detectable which binds to said second partner to form a unit, wherein;

(i) said second partner is selected from the group consisting of an energy donor and an energy acceptor, with the proviso that when said first partner is an energy donor then said second partner is an energy acceptor and when said first partner is an energy acceptor then said second partner is an energy donor, and (ii) the distance between said first partner and said second partner is 30 Angstroms or less;

c. irradiating said unit with energy that can be absorbed by said energy donor and not by said energy acceptor with the proviso that said energy donor emits fluorescent energy which can excite said energy acceptor; and

d. detecting the fluorescence emitted by said energy acceptor.

2. The method of claim 1 wherein said first partner is attached covalently to said recognition segment of said binding entity by means of a linker arm.

3. The method of claim 1 wherein said binding entity comprises an analyte-specific binding substance, said first partner is selected from the group consisting of europium and terbium, and said second partner is a fluorescent aromatic intercalating agent.

4. The method of claim 1 wherein said lanthanide metal is chelated.

5. The method of claim 1 wherein said assay is carried out in a one phase system.

6. The method of claim 1 wherein said analyte is selected from the group consisting of antigens, haptens, antibodies and target polynucleotides.

7. The method of claim 6 wherein said analyte is an antigen.

8. The method of claim 7 wherein said antigen is selected from the group consisting of proteins, polysaccharides, viruses, phages, and bacteria.

9. The method of claim 1 wherein said first partner is an energy acceptor and said second partner is an energy donor.

10. The method of claim 1 wherein said energy donor is a fluorescent aromatic agent and said energy acceptor is selected from the group consisting of fluorescent aromatic agents and lanthanide metals.

11. The method of claim 10 wherein said energy donor is a fluorescent aromatic agent and said energy acceptor is a lanthanide metal.

12. The method of claim 1 wherein aromatic agents are selected from the group consisting of auromine O, lumichrome, and 9-aminoacridine.

13. The method of claim 10 wherein said metal is selected from the group consisting of europium and terbium.

14. The method of claim 1 wherein said second partner is attached covalently or non-covalently to said component of said reporting entity by means of a linker arm.

15. The method of claim 6 wherein said analyte is selected from the group consisting of antigens and antibodies and wherein said component of said reporting entity is selected from the group consisting of Clq, antibodies, and solid supports.

16. The method of claim 15 wherein said analyte is selected from the group consisting of antigens and antibodies and wherein said component of said reporting entity is selected from the group consisting of Clq and solid supports.

17. The method of claim 6 wherein said analyte is a target polynucleotide and wherein said component of said reporting entity is selected from the group consisting of intercalating agents and solid supports.

18. The method of claim 3 wherein said solid support is selected from the group consisting of glass, plastic, cellulose, and gel polymers.

19. The method of claim 1 wherein said assay is carried out in a two phase system, wherein said two phases comprise a solid support and a liquid.

20. The method of claim 1 wherein said analyte is an antigen, said first partner is an energy acceptor, said second partner is an energy donor, and said component of said reporting entity selected from Clq and a solid support.

21. The method of claim 20 wherein said energy acceptor is a lanthanide metal.

22. The method of claim 5 wherein said analyte is an antigen, said binding entity comprises an antibody said first partner is an energy acceptor, said second partner is an energy donor, and said reporting component entity comprises Clq.

23. The method of claim 22 wherein said energy acceptor is a lanthanide metal.

24. The method of claim 1 wherein said analyte is a target polynucleotide, said recognition segment is a complementary polynucleotide, said first partner is a lanthanide metal, and said second partner is a fluorescent aromatic intercalating agent.

25. The method of claim 5 wherein said analyte is a target polynucleotide, said binding entity comprises a complementary polynucleotide, said first partner is a lanthanide metal, and said second partner is a fluorescent aromatic intercalating agent.

26. The method of claim 19 wherein said analyte is a target polynucleotide, said binding entity comprises a complementary polynucleotide, said first partner is a lanthanide metal, said second partner is a fluorescent aromatic intercalating agent.

27. The method of claim 1 wherein said analyte and said binding entity form a complex selected from the group consisting of antigen/antibody, lectin/sugar, hormone/receptor, inhibitor/enzyme, cofactor/enzyme, and ligand/substrate.

28. The method of claim 5 wherein said analyte is an antigen and wherein said binding entity comprises a specific antibody.

29. An assay for detecting the presence of an analyte comprising the steps of:

a. forming a complex comprising said analyte and a binding entity comprising an analyte recognition segment and a first partner of a fluorescent energy transfer system, wherein said first partner is selected from the group consisting of an energy donor and an energy acceptor, wherein said energy donor is a fluorescent, aromatic agent and said energy acceptor is selected from the group consisting of fluorescent, aromatic agents, europium and terbium;

b. contacting said complex with a reporting entity comprising a second partner of said fluorescent energy transfer system and a component which binds to said second partner to form a unit, wherein;

(i) said second partner is selected from the group consisting of an energy donor and an energy acceptor, with the proviso that when said first partner is an energy donor then said second partner is an energy acceptor and when said first partner is an energy acceptor then said second partner is an energy donor, and (ii) the distance between said first partner and said second partner is 30 Angstroms or less;

c. irradiating said unit with energy that can be absorbed by said energy donor and not by said energy acceptor with the proviso that said energy donor emits fluorescent energy which can excite said energy acceptor and wherein said irradiation is performed without prior separation of said binding entity which has not formed said complex with said analyte; and

d. detecting the fluorescence emitted by said energy acceptor.

30. The method of claim 29 wherein said binding entity comprises an analyte-specific binding substance, said first partner is selected from the group consisting of europium and terbium, and said second partner is a fluorescent aromatic intercalating agent.

31. The method of claim 29 wherein said assay is carried out in a two phase system, wherein said two phases comprise a solid support and a liquid.

32. The method of claim 29 wherein said europium is chelated.

33. The method of claim 29 wherein said terbium is chelated.

34. The method of claim 29 wherein said assay is carried out in a one phase system.