Apparatus and method for dilution and mixing of liquid samples

• US 4,868,129 A
• Filed: 08/27/1987
• Issued: 09/19/1989
• Est. Priority Date: 08/27/1987
• Status: Expired due to Term

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Biotrack, Inc. (Novartis Ag)
• Inventors: Gibbons, Ian, Robertson, Channing R., Hillman, Robert S.
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): Wallen, T. J.
• Application Number:
  US07/090,026
• Time in Patent Office: 754 Days
• Field of Search: 422/99, 422/100, 422/296-299, 422/102, 436/179, 436/180, 436/176, 73/864.02, 73/864.12, 73/864.52, 141/4, 141/7, 141/9, 141/285
• US Class Current: 436/179
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01F 33/452 using independent floating stirring elements
• CPC Class Codes: B01F 35/80 Forming a predetermined ratio of the substances to be mixed controlling ratio of two or more flows of fluid or fluent material G05D11/02
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: G01N 1/38 Diluting, dispersing or mixing samples
• CPC Class Codes: G01N 33/04 Dairy products
• CPC Class Codes: Y10T 436/25625 Dilution
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

An apparatus for diluting a sample with a diluent, comprising a fixed volume measuring chamber, a fixed volume receiving chamber and fluid receiving relationship to the measuring chamber, a gas vent in the receiving chamber, a stop flow junction between the measuring chamber and the receiving chamber, a sample application site in fluid donating relationship to the measuring chamber, wherein the vertical height difference between the sample application site and the stop flow junction is insufficient to provide a hydrostatic pressure capable of overcoming backpressure at the stop flow junction when sample is applied to the sample application site, and the diluent application site and fluid donating relationship to the measuring chamber. Sample and diluent are added sequentially to the apparatus of the invention without requiring any intervening operations. Various means are provided for restarting flow, which causes diluent to wash the sample into the receiving chamber, where the two can be mixed.

Claims

1. An apparatus for automatically diluting an aqueous sample with an aqueous diluent, comprising:

housing means containing

sample application site means for receiving a sample;

measuring chamber means having a first volume;

receiving chamber means having a second volume, wherein said second volume is greater than said first volume;

diluent application site means for receiving a diluent;

first flow means for delivering a sample from said sample application site means to said measuring chamber means by the sum of capillary and gravitational forces upon addition of sample to said sample application site means;

second flow means for delivering diluent from said diluent application site means to said measuring chamber means by the sum of capillary and gravitational forces upon addition of diluent to said diluent application sit means;

third flow means for delivering sample and diluent from said measuring chamber means to said receiving chamber means by the sum of capillary and gravitational forces; and

a stop flow junction located in said third flow means and adapted to the surface tension characteristics of the sample so as to provide sufficient back pressure resulting from contact between said sample and wall means of said housing means at said stop flow means to prevent sample from flowing through said stop flow junction in absence of diluent but allowing flow through said stop flow junction when diluent is present in said apparatus along with said sample,

whereby addition of diluent to said diluent application site means after sample is added to said sample application site means causes hydrostatic pressure at said stop flow junction to exceed said backpressure, whereby said diluent expels sample from said measuring chamber means and causes a fixed ratio of sample to diluent to be delivered to said receiving chamber means.

2. The apparatus of claim 1, wherein said stop flow junction comprises an increase in flow path cross-section from a capillary-flow region to a non-capillary-flow region.

3. The apparatus of claim 1, wherein said stop flow junction comprises a change in liquid contact surfaces.

4. The apparatus in claim 1, wherein said stop flow junction produces a backpressure of from 0.1 to 20 cm H₂ O when sample is present at said junction.

5. The apparatus of claim 1, wherein said measuring chamber means has a volume of from 0.1 μ

L to 100 μ

L.

6. The apparatus of claim 1, wherein said receiving chamber means has a volume of from 3 μ

L to 1000 μ

L.

7. The apparatus of claim 1, wherein said measuring chamber means has a volume of from 1 μ

L to 30 μ

L and said receiving chamber means has a volume of from 10 μ

L to 300 μ

L, with the proviso that the receiving chamber means is larger than the measuring means chamber.

8. The apparatus of claim 1, wherein the diluent application site means has a minimum volume equal to the volume of the receiving chamber means and a maximum volume of 10 mL.

9. The apparatus of claim 1, wherein the backpressure at the stop flow junction is from 0.1 to 10 cm H₂ O and the vertical height difference in cm between the minimum diluent height and the stop flow junction is greater than the numerical value in cm H₂ O of said backpressure.

10. The apparatus of claim 1, further comprising a removable, air-tight cover for said sample application site means.

11. The apparatus of claim 1, wherein said measuring chamber means is a capillary chamber.

12. The apparatus of claim 1, wherein said stop flow junction is located at the bottom of said receiving chamber means.

13. The apparatus of claim 1, wherein capillarity is present in said receiving chamber means.

15. The apparatus of claim 1, wherein a first reagent is present at a first location in said receiving chamber means and a second reagent is present at a second location in said receiving chamber means different from said first location.

16. The apparatus of claim 1, wherein said diluent application site comprises an internal chamber in said housing means and said internal chamber contains diluent, said diluent being prevented from entering said measuring chamber means by a barrier of rupturable impermeable material.

17. A method for diluting an aqueous sample with an aqueous diluent, comprising:

adding an unmeasured volume of said sample to sample application site means of a device in which said sample flows by the sum of capillary and gravitational forces to measuring chamber means having a first volume, said measuring chamber means being terminated by a stop flow junction which stops sample flow as a result of backpressure resulting from interaction of said sample with wall means of said device at said stop flow junction; and

then

adding said diluent to diluent application site means of said device, wherein said diluent flows by the sum of capillary and gravitational forces to said measuring chamber means and displaces sample into receiving chamber means having a second volume larger than said first volume when hydrostatic pressure resulting from the presence of diluent in said device exceeds said backpressure, whereby a fixed ratio sample and diluent fills said receiving chamber means.

14. The apparatus of claim 19, wherein capillarity is present throughout all internal chambers of said apparatus except at said stop flow junction.

18. The method of claim 17, further comprising sealing said sample application site means after sample has filled said measuring chamber means.

19. The method of claim 17, further comprising contacting said sample and said diluent with a first reagent at a first location in said receiving chamber means prior to contacting a second reagent at a second location in said receiving chamber.

20. The method of claim 19, wherein said sample and diluent are incubated for a specific time with said first reagent prior to contacting said second reagent, said time being determined by the rate of filling of said receiving chamber means, said rate being controlled by a flow restrictor that controls the flow of diluent from said diluent application site means.

21. The method of claim 17, wherein said stop flow junction comprises an increase in flow path cross section from a capillary-flow region to a non-capillary-flow region.

22. The method of claim 17, wherein said stop flow junction comprises a change in liquid contact surfaces.

23. The method of claim 17, wherein said stop flow junction produces a back pressure of from 0.1 to 20 cm H₂ O.

24. The method of claim 17, wherein said measuring chamber means has a volume of from 0.1 μ

L to 100 μ

L.

25. The method of claim 17, wherein said receiving chamber means has a volume of from 3 μ

L to 1,000 μ

L.

26. The method of claim 17, wherein said diluent application site means has a minimum volume equal to the volume of the receiving chamber means and a maximum volume of 10 mL.

27. The method of claim 17, wherein said measuring chamber means is a capillary chamber.