Light collecting device with chamber including ellipsoidal surface and spherical surface

• US 4,871,249 A
• Filed: 07/01/1988
• Issued: 10/03/1989
• Est. Priority Date: 07/10/1987
• Status: Expired due to Fees

• Current Assignee: Medical Research Council (Government of United Kingdom)
• Original Assignee: Medical Research Council (Government of United Kingdom)
• Inventors: Watson, James V.
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US07/214,192
• Time in Patent Office: 459 Days
• Field of Search: 350/619, 350/630, 356/73, 356/72, 356/338, 356/318
• US Class Current: 356/73
• CPC Class Codes: G01N 15/1436 the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/6469 Cavity, e.g. ellipsoid
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 2201/065 Integrating spheres
• CPC Class Codes: G02B 17/086 wherein the system is made of a single block of optical material, e.g. solid catadioptric systems
• CPC Class Codes: G02B 19/0023 at least one surface having optical power
• CPC Class Codes: G02B 19/0033 characterised by the use
• CPC Class Codes: G02B 19/008 adapted to collect light from a complete hemisphere or a plane extending 360 degrees around the detector
• CPC Class Codes: G02B 5/10 with curved faces

Abstract

A light collecting device for flow cytometry comprises an internally reflecting chamber for collecting light from a point source within the chamber over a solid angle of substantially 4 pi and directing all the collected light through an exit point at or adjacent the wall of the chamber. The chamber comprises an ellipsoidal surface extending over a solid angle of substantially 2 pi and a spherical surface extending over a solid angle of substantially 2 pi, the firsts conjugate focus of the ellipsoidal surface being coincident with the center of curvature of the spherical surface and the second conjugate focus of the ellipsoidal surface being at the center point of the spherical surface. A capillary bore passes through the first conjugate focus to accommodate a sample stream which is illuminated by laser light to produce fluorescence at the first conjugate focus.

Claims

1. A light collecting device comprising an internally reflecting chamber for collecting light from a point source within the chamber over a solid angle of substantially 4 pi and directing all the collected light through an exit point at or adjacent the wall of the chamber, said chamber being in the form of an ellipsoidal surface extending over a solid angle of substantially 2 pi and a spherical surface extending over a solid angle of substantially 2 pi, the first conjugate focus of the ellipsoidal surface being coincident with the centre of curvature of the spherical surface and the second conjugate focus of the ellipsoidal surface being located at or adjacent the spherical surface at the centre point of the curved surface area thereof, whereby in use light from a point source located at the said first conjugate focus is directed through the said second conjugate focus, some by virtue of a single reflection at the ellipsoidal surface and the rest by virtue of an initial reflection at the spherical surface followed by a reflection at the ellipsoidal surface.

2. A light collecting device according to claim 1, wherein the ellipsoidal and spherical surfaces are connected through a waist which is used for mounting the device.

3. A light collecting device according to claim 2, wherein the waist is an axially-short cylindrical surface.

4. A light collecting device according to claim 1, wherein a polished light exit pupil is provided in the region of the exit point, cut into the spherical surface with a centre of curvature at the exact exit point, so that all light rays reflected from the ellipsoidal surface are normal to the surface of the pupil, whereby the device is non-refracting and hence completely achromatic.

5. A light collecting device according to claim 1, wherein a capillary bore passes through the said first conjugate focus to accommodate a sample stream for flow cytometry.

6. A light collecting device according to claim 5, wherein illumination to produce fluorescence at the said first conjugate focus is by way of laser light directed through a waist interconnecting the ellipsoidal and spherical surface, or arc-lamp light directed through the exit point to be focussed at the first conjugate focus.

7. A light collecting device according to claim 5, wherein the chamber is formed from laser grade fused silica or quartz, and incorporates integral end discs which are interconnected by the capillary bore which passes through the first conjugate focus, which is coincident with the centre of curvature of the spherical surface.

8. A light collecting device according to claim 7, wherein the chamber is silvered except at the waist and exit pupil, and the silvered surface is black lacquer coated for protection.