Isolation of exogenous recombinant proteins from the milk of transgenic mammals
First Claim
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1. A process for the production and secretion into mammal'"'"'s milk of an exogenous recombinate protein comprising the steps of:
- a. producing milk in a transgenic mammal characterized by an expression system comprising a casein promoter operatively linked to an exogenous DNA sequence coding for the recombinant protein through a DNA sequence coding for a signal peptide effective in secreting and maturing the recombinant protein in mammary tissue;
b. collecting the milk; and
c. isolating the exogenous recombinant protein from the milk.
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Abstract
This invention relates to the production of recombinant proteins in mammals'"'"' milk. Particularly, this invention relates to an expression system comprising the mammal'"'"'s casein promoter which when transgenically incorporated into a mammal permits the female species of that mammal to produce the desired recombinant protein in or along with its milk. This invention also relates to the transgenic mammal that produces the desired recombinant product in its milk.
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3 Claims
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1. A process for the production and secretion into mammal'"'"'s milk of an exogenous recombinate protein comprising the steps of:
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a. producing milk in a transgenic mammal characterized by an expression system comprising a casein promoter operatively linked to an exogenous DNA sequence coding for the recombinant protein through a DNA sequence coding for a signal peptide effective in secreting and maturing the recombinant protein in mammary tissue; b. collecting the milk; and c. isolating the exogenous recombinant protein from the milk. - View Dependent Claims (2, 3)
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