Process for producing tryptophan

• US 4,874,698 A
• Filed: 07/16/1987
• Issued: 10/17/1989
• Est. Priority Date: 02/17/1983
• Status: Expired due to Term

• Current Assignee: Kyowa Hakko Kogyo Company Limited (Kirin Holdings Company Limited)
• Original Assignee: Kyowa Hakko Kogyo Company Limited (Kirin Holdings Company Limited)
• Inventors: Oka, Tetsuo, Ozaki, Akio, Katsumata, Ryoichi
• Primary Examiner(s): Huleatt, Jayme A.
• Application Number:
  US07/073,888
• Time in Patent Office: 824 Days
• Field of Search: 435/108, 435/172.3, 435/252.32, 435/320, 435/840, 435/843, 935/60, 935/72
• US Class Current: 435/108
• CPC Class Codes: C12N 15/52 Genes encoding for enzymes or proenzymes
• CPC Class Codes: C12P 13/04 Alpha- or beta- amino acids other amino acids C12P13/005
• CPC Class Codes: C12P 13/06 Alanine; Leucine; Isoleucine; Serine; Homoserine
• CPC Class Codes: C12P 13/10 Citrulline; Arginine; Ornithine
• CPC Class Codes: C12P 13/22 Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
• CPC Class Codes: Y10S 435/84 Brevibacterium
• CPC Class Codes: Y10S 435/843 Corynebacterium

Abstract

Disclosed is a process for producing tryptophan by transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA of a DNA fragment containing a gene involved in the biosynthesis of tryptophan and a vector DNA, culturing the transformant in a nutrient medium, accumulating tryptophan in the culture medium and recovering tryptophan therefrom.

Claims

1. A process for producing tryptophan, which comprises:

transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a vector containing a DNA fragment containing an anthranilic acid synthetase gene isolated from Brevibacterium flavum ATCC 14067 or Corynebacterium glutamicum ATCC 13032, culturing the transformant in a nutrient medium containing a molasses carbon source, accumulating tryptophan in the culture medium and recovering tryptophan therefrom.

2. The process according to claim 1, wherein the host microorganism belongs to the genus Corynebcterium or Brevibacterium and is sensitive to lysozyme.

3. A process for producing tryptophan, which comprises:

culturing in a nutrient medium containing a molasses carbon source a microorganism belonging to the genus Corynebacterium or Brevibacterium comprising a vector containing a DNA fragment encoding an anthranilic acid synthetase insensitive to tryptophan, which fragment was isolated fom Brevibacterium flavum ATCC 14067 or Corynebacterium glutamicum ATCC 13032, accumulating tryptophan in the culture medium and recovering tryptophan therefrom.

4. A microorganism belonging to the genus Corynebacterium or Brevibacterium and comprising a vector containing a DNA fragment encoding an anthranilic acid synthetase insensitive to tryptophan, which fragment was isolated from Brevibacterium flavum ATCC 14067 or Corynebacterium glutamicum ATCC 13032.

5. A micoorganism belonging to the genus Corynebacterium or Brevibacterium comprising vector containing a DNA fragment containing an anthranilic acid synthetase gene isolated from Brevibacterium flavum ATCC 14067 or Corynebacterium glutamicum ATCC 13032.

6. A biologically pure culture of Corynebacterium glutamicum K20, ATCC 39035, which contains plasmid pTrp 2-3.

7. A biologically pure culture of Corynebacterium glutamicum K31, ATCC 39280, which contains plasmid pTrp 4-3.

8. A biologically pure culture of Corynebacterium glutamicum K37, ATCC 39285, which contains plasmid pTrp 13-2.