Compositions for the detection of high levels of hydrogen peroxide

• US 4,876,207 A
• Filed: 02/16/1988
• Issued: 10/24/1989
• Est. Priority Date: 02/16/1988
• Status: Expired due to Term

• Current Assignee: Clinical Diagnostic Systems Inc.
• Original Assignee: Eastman Kodak Company
• Inventors: Mack, Arthur R. II, Sutherland, John W. H. Jr.
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US07/156,129
• Time in Patent Office: 616 Days
• Field of Search: 435/4, 435/10, 435/11, 435/12, 435/14, 435/22, 435/25, 435/26, 435/28, 435/171, 435/189, 435/190, 435/192, 435/805, 435/810, 435/911, 436/71, 436/95, 436/97, 436/99, 436/169, 436/170, 436/163, 436/175, 436/903, 436/135, 436/63, 436/171, 422/55-58, 252/186.1, 252/186.2, 252/186.25, 252/186.28, 252/188.1, 252/408.1
• US Class Current: 436/135
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72
• CPC Class Codes: G01N 33/526 the element being adapted for a specific analyte
• CPC Class Codes: Y10T 436/104998 Glucose, ketone, nitrate standard or control
• CPC Class Codes: Y10T 436/206664 Ozone or peroxide

Abstract

A dye development composition comprising 1,10-phenanthroline and 1,7-dihydroxynaphthalene is disclosed for use in assaying high levels of hydrogen peroxide.

Claims

1. A dye development composition comprising 1,10-phenanthroline and 1,7-dihydroxynaphthalene.

2. The composition of claim 1 also comprising a peroxidase active material.

3. The composition of claim 1 or 2 wherein each chemical component is present in a sufficient concentration to at least react stoichiometrically with an expected concentration of an analyte.

4. A method for determining hydrogen peroxide levels in aqueous solution having a pH comprising the steps of:

(a) adjusting the pH of the aqueous solution between 5 and 10;

(b) providing a peroxidase active material;

(c) providing a composition comprising 1,10-phenanthroline and 1,7-dihydroxynaphthalene;

(d) combining (a), (b) and (c) to form a dye; and

(e) determining the hydrogen peroxide level photometrically from the dye.

5. The method of claim 4 wherein the photometric determinations are carried out at wavelengths greater than 620 nanometers.

6. The method of claim 4 wherein the photometric determination is carried out at about 670 nanometers.

8. The method as in claim 4 or 7 wherein the aqueous solution is blood serum.

7. A method for determining glucose levels in an aqueous solution having a pH comprising the steps of:

(a) adjusting the pH of the aqueous solution between 5 and 10;

(b) providing a peroxidase active material;

(c) providing a composition comprising 1,10-phenanthroline and 1,7-dihydroxynaphthalene;

(d) combining a), b) and c) to form a dye; and

(e) determining the glucose level photometrically from the dye.

9. An analytical element for determining hydrogen peroxide levels in aqueous fluids comprising an absorbent material containing a peroxidase active material;

1,10-phenanthroline and 1,7-dihydroxynaphthalene.

10. A multilayer analytical element for determination of hydrogen peroxide in an aqueous medium comprising a support bearing in the following order;

(a) one or more reagent layer(s) comprising a peroxidase active substance, 1,10-phenanthroline and 1,7-dihydroxynaphthalene;

wherein the peroxidase active substance and 1,10-phenanthroline and 1,7-dihydroxynaphthalene are in the same reagent layers; and

(b) a spreading layer in fluid contact with the reagent layer(s).

11. The element of claim 10 comprising two or more reagent layers wherein one of the reagent layers comprises a material which oxidizes glucose to produce hydrogen peroxide and the second reagent layer comprises 1,10-phenanthroline and 1,7-dihydroxynaphthalene.

12. The element of claim 10 comprising a combination spreading and reagent layer in contact with a second reagent layer.

13. The element of claim 12 wherein a) the spreading and reagent layer comprises from 1 to 8 g/m² of NaH₂ PO₄ ;

from 2 to 5 g/m² of polyvinyl pyrrolidone-K90 reagent;

from 10,000 to 50,000 I.U. of peroxidase;

from 10,000 to 50,000 I.U. of glucose oxidase and from 0.05 to 0.5 g/m² of a non ionic surfactant; and

(b) the reagent layer comprises from 0.5 to 1.5 g/m² 1,10-phenanthroline;

from 0.5 to 1.5 g/m² 1,7-dihydroxynaphthalene;

from 1 to 5 g/m² of polyvinyl pyrrolidone-K90 reagent;

from 0.1 to 3 g/m² of poly(styrene-co-m+p-N-vinyl-benzyl-N-benzyl-N,N-dimethyl ammonium chloride (60,40)-co-divinylbenzene (49.5;

49.5;

     1) and from 0.05to -5 g/m² of a non ionic surfactant.

14. A multilayer analytical element for determination of hydrogen peroxide in an aqueous medium comprising a support bearing in the following order;

(a) one or more reagent layer(s) comprising a peroxides active substance, 1, 10-phenanthroline and 1,7-dihydroxynaphthalene;

wherein the peroxidase active substance and 1,10-phenanthroline and 1,7-dihydroxynaphthalene are in different reagent layers; and

(b) a spreading layer in fluid contact with the reagent layer(s).