Poly-labelled oligonucleotide derivative
First Claim
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1. A poly-labelled oligonucleotide derivative represented by the following formula [I]:
- ##STR5## wherein;
either one of R1 and R2 is a group represented by the formula [II] shown below and the other is a hydrogen atom or a phosphate group, or both of R1 and R2 are groups represented by the formula [II] shown below;
n is a natural number from 6 to 200; and
B is nucleotide base selected from the group consisting of adenine, thymine, cytosine and guanine;
##STR6## wherein;
a is a natural number from 2 to 50 and b is a natural number from 1 to 50, with b+1≧
a;
R3 is an alkylene group having 2 to 20 carbon atoms;
X represents an oxygen atom or an imino group;
(Lys)b represents a lysine moiety when b=1 and a polylysine moiety when b=2 to 50, wherein said polylysine is a polymerization product of lysine through the reaction of its amino group and its carboxylic group with the number of molecules of lysine polymerized therein being equal to b, and wherein (Lys)b is bonded to the moiety --NH-- in formula [II] through reaction of the carboxylic group in the (Lys)b moiety;
Y is a label which is selected from the group consisting of biotin, haptens, fluorophores, and chemiluminescent substances, and which is covalently bonded to the (Lys)b moiety through reaction with the amino group or groups in the (Lys)b moiety.
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Abstract
A poly-labelled oligonucleotide having a plurality of labels, comprising labels carried on a polylysine and having the polylysine introduced on the extension from 5'"'"'- and/or 3'"'"'-end through phosphate group is useful as a probe for hybridization.
209 Citations
21 Claims
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1. A poly-labelled oligonucleotide derivative represented by the following formula [I]:
- ##STR5## wherein;
either one of R1 and R2 is a group represented by the formula [II] shown below and the other is a hydrogen atom or a phosphate group, or both of R1 and R2 are groups represented by the formula [II] shown below;
n is a natural number from 6 to 200; and
B is nucleotide base selected from the group consisting of adenine, thymine, cytosine and guanine;
##STR6## wherein;
a is a natural number from 2 to 50 and b is a natural number from 1 to 50, with b+1≧
a;
R3 is an alkylene group having 2 to 20 carbon atoms;
X represents an oxygen atom or an imino group;
(Lys)b represents a lysine moiety when b=1 and a polylysine moiety when b=2 to 50, wherein said polylysine is a polymerization product of lysine through the reaction of its amino group and its carboxylic group with the number of molecules of lysine polymerized therein being equal to b, and wherein (Lys)b is bonded to the moiety --NH-- in formula [II] through reaction of the carboxylic group in the (Lys)b moiety;
Y is a label which is selected from the group consisting of biotin, haptens, fluorophores, and chemiluminescent substances, and which is covalently bonded to the (Lys)b moiety through reaction with the amino group or groups in the (Lys)b moiety. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
- ##STR5## wherein;
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Current AssigneeWakunaga Seiyaku Kabushiki Kaisha
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Original AssigneeWakunaga Seiyaku Kabushiki Kaisha
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InventorsKawasoe, Tatsuro, Tsukumo, Noriko, Miyoshi, Kenichi, Yamane, Akio
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Primary Examiner(s)Rollins, John W.
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Application NumberUS07/067,798Time in Patent Office847 DaysField of Search536/27, 536/28, 536/29US Class Current536/24.3CPC Class CodesC07H 21/00 Compounds containing two or...C12Q 1/689 for bacteria
