Apparatus for preparative gel electrophoresis

• US 4,877,510 A
• Filed: 10/25/1988
• Issued: 10/31/1989
• Est. Priority Date: 10/25/1988
• Status: Expired due to Term

• Current Assignee: Bio-Rad Laboratories Incorporated
• Original Assignee: Bio-Rad Laboratories Incorporated
• Inventors: Chen, Jin-Hai
• Primary Examiner(s): Niebling, John F.
• Assistant Examiner(s): Starsiak, Jr., John S.
• Application Number:
  US07/262,905
• Time in Patent Office: 371 Days
• Field of Search: 204/182.8, 204/299 R, 204/301
• US Class Current: 204/613
• CPC Class Codes: B01D 57/02 by electrophoresis treatment of water, waste water, sewage or sludge by electrophoresis C02F1/469; electrophoretic production of compounds or non-metals C25B7/00; investigating or analysing materials by using electrophoresis G01N27/26
• CPC Class Codes: G01N 27/44717 Arrangements for investigating the separated zones, e.g. localising zones

Abstract

Emerging solutes from a preparative gel electrophoresis column are drawn into a narrow transfer tube through a collar which fits over the column end and secures a porous plate and dialysis membrane to the column end. The collar is provided with a series of channels running from the collar exterior to a series of locations on its interior wall based around the periphery of the porous plate. An external pump draws lower buffer solution into these channels and laterally through the porous plate to essentially located withdrawal tubing. The solutes emerging from the tubing are then directed to a fraction collector.

Claims

1. Apparatus for drawing solutes eluting from an open-ended tubular electrophoresis column having a given cross section into transfer tubing, said apparatus comprising

a porous plate and a dialysis membrane, both sized to span said cross section, said dialysis membrane being impermeable to said solutes;

a collar adapted to receive said open end and to secure said porous plate and said dialysis membrane thereto;

a plurality of channels in said collar communicating the exterior of said collar with a plurality of loci along the interior wall thereof, said loci positioned to reside along the periphery of said open end of said tubular electrophoresis column when said collar is joined thereto; and

means for drawing fluid from said porous plate at a withdrawal site within the periphery thereof.

2. Apparatus in accordance with claim 1 in which said loci are positioned at substantially regular intervals around said periphery.

3. Apparatus in accordance with claim 1 further comprising means for clamping said collar to said open-ended tubular electrophoresis column.

4. Apparatus in accordance with claim 1 in which said channels are furrows along the interior wall of said collar.

5. Apparatus in accordance with claim 4 in which said open-ended tubular electrophoresis column has an axis and said furrows are parallel to said axis.

6. Apparatus in accordance with claim 4 in which said furrows are spaced at substantially regular intervals along said interior wall.

7. Apparatus in accordance with claim 4 in which said furrows are at least six in number.

8. Apparatus in accordance with claim 4 in which said furrows are at least twelve in number.

9. Apparatus in accordance with claim 1 in which said withdrawal site is approximately at the center of said porous plate.

10. Apparatus in accordance with claim 1 in which said fluid drawing means draws fluid in a direction parallel to the axis of said open-ended tubular electrophoresis column.

11. Apparatus in accordance with claim 10 in which said direction is away from the interior of said open-ended tubular electrophoresis column.

12. Apparatus in accordance with claims 1 or 10 in which said dialysis membrane has an opening to permit passage of said solutes therethrough and said direction is away from the interior of said open-ended tubular electrophoresis column through said opening.

13. Apparatus in accordance with claim 10 in which said fluid drawing means draws fluid through the interior of said open-ended tubular electrophoresis column.

14. Apparatus for preparative electrophoresis, comprising:

a gel enclosure open at opposite ends;

upper and lower buffer chambers and means for imposing an electric potential therebetween, said upper buffer chamber adapted to contain an upper buffer solution and to place said upper buffer solution in fluid contact with a first open end of said gel enclosure, and said lower buffer chamber sized to contain a lower buffer solution and to permit immersion of a second open end of said gel enclosure therein;

a porous plate and a dialysis membrane, both sized to span said second open end, said dialysis membrane being impermeable to said solutes;

a collar adapted to receive said second open end and to secure said porous plate and said dialysis membrane thereto;

a plurality of channels in said collar communicating the exterior of said collar with a plurality of loci along the interior wall thereof, said loci position to reside along the periphery of said second open end of said tubular electrophoresis column when said collar is joined thereto; and

means for drawing fluid from the interior of said collar at a withdrawal site within the periphery of said porous plate to a location outside said lower buffer chamber.

15. Apparatus in accordance with claim 14 in which said channels are furrows along the interior wall of said collar.

16. Apparatus in accordance with claim 15 in which said furrows are spaced at substantially regular intervals along said interior wall.

17. Apparatus in accordance with claim 14 in which said collar is shaped to receive said second open end of said gel enclosure in a friction fit.

18. Apparatus in accordance with claim 14 in which said withdrawal site is approximately at the center of said porous plate.

19. Apparatus in accordance with claim 14 in which said gel enclosure has a central axis and said fluid drawing means draws fluid in a direction parallel to said central axis.

20. Apparatus in accordance in claim 19 in which said direction is away from the interior of said gel enclosure when said collar is joined thereto.

21. Apparatus in accordance with claim 19 in which said dialysis membrane has an opening to permit passage of said solutes therethrough and said direction is away from the interior of said gel enclosure through said opening.

22. Apparatus in accordance with claim 19 in which said fluid drawing means draws fluid through the interior of said gel enclosure.