Apparatus and process for reagent fluid dispensing and printing

• US 4,877,745 A
• Filed: 03/14/1989
• Issued: 10/31/1989
• Est. Priority Date: 11/17/1986
• Status: Expired due to Term

• Current Assignee: Abbott Laboratories
• Original Assignee: Abbott Laboratories
• Inventors: Verlee, Donald J., Houseman, Kenneth R., Wallace, David B., Hayes, Donald J.
• Primary Examiner(s): LUSIGNAN, MICHAEL R
• Application Number:
  US07/325,037
• Time in Patent Office: 231 Days
• Field of Search: 346/1.1, 346/140 R, 422/55, 422/56, 422/58, 422/61, 436/166, 436/180
• US Class Current: 436/166
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00603 Making arrays on substantially continuous surfaces
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 4/02 for feeding measured , i.e. prescribed quantities of reagents
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01F 11/08 of the diaphragm or bellows type
• CPC Class Codes: G01F 11/088 using a deformable conduit-like element
• CPC Class Codes: G01N 2035/1041 Ink-jet like dispensers
• CPC Class Codes: G01N 35/1095 for supplying the samples to flow-through analysers for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

This invention relates to a system for printing and dispensing chemical reagents in precisely controlled volumes onto a medium at a precisely controlled location. A jetting tube, comprising an orifice at one end and a fluid receiving aperture at the other end, is concentrically mounted within a cylindrical piezo-electric transducer. The fluid receiving aperture is connected to a reservoir containing a selected reagent by means of a filter. The reservoir may be pressurized by a regulated air supply. An electrical signal of short duration is applied to the transducer. The pulse causes the transducer and the volume defined by the jetting tube to expand, thereby drawing in a small quantity of reagent fluid. The cessation of the pulse causes the transducer and the volume of the jetting tube to de-expand, thereby causing at least a substantially uniformly sized droplet of reagent fluid to be propelled through the orifice. The droplet may be directed to impact a printing medium or collected in a dispensing recepticle.

Claims

1. A diagnostic analyzing system comprising:

means for positioning and containing at least one of (1) a diagnostic reagent fluid and (2) a sample; and

means for precisely metering and dispensing the other one of the (1) diagnostic reagent fluid and (2) sample to interact with the positioned one of the (1) diagnostic reagent fluid and (2) sample, the metering and dispensing means comprising;

a jetting chamber defining a volume and comprising a first and second aperture, the first aperture adapted to receive the other one of the (1) diagnostic reagent fluid and (2) sample, the second aperture defining an orifice;

a transducer in mechanical communication with the jetting chamber, the transducer operative to alternately expand and de-expand the volume of the jetting chamber in response to a selected electrical pulse and thereby cause the jetting chamber to propel a reproducibly sized droplet of the other one of the (1) diagnostic reagent fluid and (2) sample from the orifice;

means for generating a number of electrical pulses sufficient to cause a precise quantity of the other one of the (1) diagnostic reagent fluid and (2) sample to be dispensed; and

means for detecting reaction results produced by the interaction of the diagnostic reagent fluid and sample.

2. The invention of claim 1 wherein the fluid is an analytic reagent.

3. The invention of claim 1 wherein the system further comprises means for incubating at least one of the fluid and sample at a predetermined temperature for a predetermined time period.

4. The invention of claim 1 wherein at least one of the fluid and sample is a biologically active reagent.

5. The invention of claim 1 wherein the means for generating the electrical pulse comprises means for adjusting the electrical characteristics of the pulse in accordance with selected variable macroscopic properties of the other one of the (1) diagnostic reagent fluid and (2) sample.

6. The invention of claim 1 wherein the transducer is one of (1) a piezo-electric transducer;

(2) a magneto-strictive transducer;

(3) an electro-strictive transducer; and

(4) an electro-mechanical transducer.

7. The invention of claim 1 wherein the orifice comprises an end face and the end face is coated with a hydrophobic material.

15. The invention of claim 1 wherein the sample is a fluid.

16. The invention of claim 1 wherein the system further comprises means for pressurizing the other one of the (1) diagnostic reagent fluid and (2) sample, the means for pressurizing and the transducer cooperating to produce a stream of distinct reproducibly sized droplets.

8. A method of assaying for an analyte comprising the steps of:

(1) dispensing precise quantities of at least one diagnostic reagent fluid and at least one fluid sample by;

(a) generating at least one electrical pulse of selected predefined characteristics;

(b) altering the volume of a chamber containing the fluid by electro-mechanical means in response to the electrical pulse such that at least one droplet of fluid of a selected predetermined and reproducible volume is jetted from the chamber; and

(c) repeating steps (a) and (b) until a desired quantity of fluid has been dispensed; and

(2) detecting a reaction result produced by the fluid mixture.

9. The invention of claim 8 wherein the method further comprises the step of incubating at least one of the fluids at a predetermined temperature for a predetermined time period.

10. A method of manufacturing a diagnostic test device, the test device of the type including a chemically treated test medium, the method comprising the steps of:

(a) positioning a test device medium relative to a jetting chamber;

(b) generating at least one electrical pulse of selected predetermined characteristics;

(c) altering the volume of a chamber containing a reagent fluid by electro-mechanical means in response to the selected electrical pulse such that reagent fluid jetted from the chamber is in the form of a predetermined and reproducibly sized droplet and impacts the test device medium; and

(d) repeating steps (a) through (c) until a desired pattern of reagent fluid has been formed on the test device medium.

11. A diagnostic test device manufactured in accordance with the method of claim 10.

12. A diagnostic test device manufactured in accordance with the method of claim 10 and wherein the pattern corresponds to at least one alphanumeric character.

17. The invention of claim 10 wherein the system further comprises means for pressurizing the fluid, the means for pressurizing and the transducer cooperating to produce a stream of distinct reproducibly sized droplets.

13. A diagnostic fluid dispensing apparatus comprising:

a jetting chamber comprising an orifice and a fluid intake aperture;

a collapsible fluid reservoir containing at least one diagnostic reagent fluid connected to the intake aperture of the jetting chamber; and

a transducer adapted to alternately expand and de-expand the jetting chamber to expel fluid droplets;

the jetting chamber, reservoir and transducer cooperating such that capillary action at the orifice produces a pressure differential between the interior and exterior of the fluid reservoir upon emittance of fluid from the jetting chamber.

18. The invention of claim 13 wherein the diagnostic reagent fluid is a biologically active reagent.

14. A method of manufacturing a diagnostic test device comprising the steps of:

(a) positioning a test device medium relative to a jetting chamber;

(b) applying pressurized diagnostic reagent fluid to a jetting chamber with an orifice such that fluid is expelled through the orifice;

(c) altering the volume of the chamber by electro-mechanical means such that the expelled fluid is in the form of a stream of distinct uniformly sized droplets; and

(d) directing at least one of the (1) expelled fluid and (2) the test device medium such that the fluid impacts the medium in a desired manner.