Optical assay: method and apparatus

• US 4,877,747 A
• Filed: 04/04/1986
• Issued: 10/31/1989
• Est. Priority Date: 04/12/1985
• Status: Expired due to Fees

• Current Assignee: Plessey Overseas Limited (Marconi Holdings Company Limited)
• Original Assignee: Plessey Overseas Limited (Marconi Holdings Company Limited)
• Inventors: Stewart, William J.
• Primary Examiner(s): Hill, Jr., Robert J.
• Application Number:
  US06/848,679
• Time in Patent Office: 1,306 Days
• Field of Search: 436/164, 436/525, 436/805, 436/807, 422/55, 422/57, 422/58, 422/68, 422/69, 350/96.12, 350/96.15, 350/96.17, 350/96.19, 350/96.34, 435/287, 435/291, 435/808
• US Class Current: 436/525
• CPC Class Codes: G01N 2021/651 Cuvettes therefore
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: G01N 21/554 detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures

Abstract

An optical assay apparatus of the type comprising an optic waveguide and a coating sensitized to a specific assay species. The light signal response of the apparatus is enhanced by coupling a resonant metallic medium to the waveguide and to the sensitized coating. To this end, a metal coated transparent buffer layer may be interposed between the waveguide and the sensitized coating. Alternatively, a metallized optically matched grating can be used in place of the buffer layer or the metallic medium can be of particulate form, with each particle having a sensitized coating.

In using the optical assay apparatus, the sensitized coating is exposed to a fluid assay sample. A light beam is directed onto the sensitized coating and reflected onto a detector. The detected light is monitored for any change indicating the presence of the specific assay species in the fluid sample.

Claims

1. An optical assay method comprising the following steps:

providing a coated metallic medium, the coating thereof being sensitized for a specific assay species;

generating in the metallic medium a resonant signal at optical frequency, the power of this signal extending into the sensitized coating;

providing coupling between said resonant signal and a monitored optical beam;

exposing the coated metallic medium to a fluid assay sample; and

,

measuring any changes in the optical beam to detect a response to the presence of the specific assay species in the fluid sample.

2. An optical assay apparatus comprising:

a light source;

a light detector;

a propagating medium arranged relative to the source and detector for directing light from the source onto the detector;

a buffer means of optically less dense material than that of the propagating medium adjacent to a surface of the propagating medium;

a metallic medium, having the form of a metal film, on the surface of the buffer medium remote from the propagating medium; and

a coating, adjacent to the metallic medium, sensitized for a specific assay species.

3. An optical assay apparatus comprising:

a light source;

a light detector;

a propagating medium arranged relative to the source and detector for directing light from the source onto the detector;

an optical grating at a surface of the propagating medium;

a metallic medium, having the form of a metal film, on a surface of the optical grating; and

a coating, adjacent to the metallic medium, sensitized for a specific assay species.

4. An optical assay apparatus comprising:

a light source;

a light detector;

a propagating medium, arranged relative to the source and the detector, for directing light from the source onto the detector;

a dispersion of fine metal particles, each coated with a material sensitized for a specific assay species; and

said propagating medium and dispersion of fine metal particles being positioned relative to one another to provide optical coupling therebetween.

5. An optical assay apparatus comprising:

a light source;

a light detector;

a light propagating medium having a reflecting surface, said reflecting surface being arranged relative to the source and the detector for directing light from the source onto the detector;

a coated metallic medium arranged in close proximity to said reflecting surface, said metallic medium having a coating sensitized for a specific assay species; and

said propagating medium and metallic medium being positioned relative to one another to provide optical coupling therebetween.

6. Apparatus as claimed in claim 5, wherein the reflecting surface has the form of an optical grating, and the metallic medium has the form of a metal film on a surface of said optical grating.

7. An optical assay apparatus comprising:

a light source;

a light detector;

a light propagating medium having a reflecting surface, said reflecting surface being arranged relative to the source and the detector for directing light from the source onto the detector;

a coating metallic medium arranged in close proximity to said reflecting surface, said metallic medium having a coating sensitized for a specific assay species; and

said coated metallic medium having the form of a continuous thin metal film spaced from the reflecting surface of the light propagating medium by a buffer layer of material optically less dense than that of the light propagating medium.

8. An optical assay apparatus comprising:

a light source;

a light detector;

a light propagating medium having a reflecting surface, said reflecting surface being arranged relative to the source and the detector for directing light from the source onto the detector; and

a coating metallic medium arranged in close proximity to said reflecting surface, said metallic medium having a coating sensitized for a specific assay species; and

said metallic medium having the form of a dispersion of metal particles, with a multiplicity of the metal particles lying upon the reflecting surface.

9. An optical assay method comprising the following steps:

providing a light propagating medium having a reflecting surface, said reflecting surface arranged to direct light from a source onto a detector;

providing a coated metallic medium arranged in close proximity to said reflecting surface, said coated metallic medium having a coating sensitized for a specific assay species;

providing optical coupling between the coated metallic medium and the directed light to generate in the coated metallic medium a resonant signal at optical frequency with the power of said signal extending into the coating;

exposing the coated metallic medium to a fluid assay sample; and

measuring any changes in the directed light to detect a response to the presence of the specific assay species in the fluid assay sample.

10. An optical assay method as claimed in claim 9 further comprising the step of providing a buffer medium of optically less dense material than said propagating medium between said propagating medium and said coated metallic medium.

11. An optical assay method as claimed in claim 9 further comprising the step of providing an optical grating as said reflecting surface.

12. An optical assay method as claimed in claim 9 further comprising the step of laying a multiplicity of metal particles upon the reflecting surface as said coated metallic medium.