Metal chelate resins

• US 4,877,830 A
• Filed: 07/13/1987
• Issued: 10/31/1989
• Est. Priority Date: 07/10/1986
• Status: Expired due to Term

• Current Assignee: Roche Diagnostic Systems Incorporated (Roche Holding AG)
• Original Assignee: Hoffmann-La Roche Incorporated (Roche Holding AG)
• Inventors: Dobeli, Heinz, Hochuli, Frich
• Primary Examiner(s): Kight, John
• Assistant Examiner(s): Nutter, Nathan M.
• Application Number:
  US07/072,452
• Time in Patent Office: 841 Days
• Field of Search: 525/54.1, 525/54.3, 525/54.31, 525/54.32, 525/54.4, 525/54.45
• US Class Current: 525/54.3
• CPC Class Codes: B01D 15/3828 Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
• CPC Class Codes: B01J 20/3265 with an organic functional group containing a metal, e.g. a metal affinity ligand
• CPC Class Codes: C07K 1/22 Affinity chromatography or related techniques based upon selective absorption processes

Abstract

Metal chelate resins whose complexed nitrilotriacetic acid residues are bound to a carrier matrix via a spacer and which are suitable for metal chelate chromatography of proteins, especially those which contain neighboring histidines.

Claims

1. A metal chelate resin of the formula:

space="preserve" listing-type="equation">carrier matrix-spacer--NH--(CH.sub.2).sub.x --CH(COOH)--N(CH.sub.2 COO.sup.-).sub.2 Ni.sup.2+

wherein X=2-4.

2. The metal chelate resin of claim 1 wherein the carrier matrix is cross-linked agarose.

3. The metal chelate resin of claim 2 wherein the spacer is --O--CO--.

4. A method for the manufacture of a metal chelate resin comprising the steps of:

a) reacting a carrier matrix with a spacer;

b) reacting the carrier matrix-spacer complex with a compound of the formula;

space="preserve" listing-type="equation">H.sub.2 N--(CH.sub.2).sub.X --CH(COOH)--N(CH.sub.2 COOH).sub.2

wherein X=2-4; and

c) washing with a nickel salt solution.

5. The method of claim 8, wherein the carrier matrix-spacer complex is oxirane-agarose which contains ##STR3## groups.

6. The method of claim 8 wherein the fuctionalized carrier matrix-spacer complex is imidazolecarbamate-agarose which contains ##STR4## groups.