Apparatus for determining base sequence

• US 4,881,812 A
• Filed: 12/07/1987
• Issued: 11/21/1989
• Est. Priority Date: 03/31/1987
• Status: Expired due to Term

• Current Assignee: Shimadzu Corporation 1 Nishinokyo Kuwabaracho Nakagyo-Ku Kyoto Japan
• Original Assignee: Shimadzu Corporation
• Inventors: Ohkubo, Kunihiko, Fujii, Hidehiko
• Primary Examiner(s): Rosenberger, Richard A.
• Application Number:
  US07/129,295
• Time in Patent Office: 715 Days
• Field of Search: 356/344, 356/417, 356/431, 250/461.2, 250/563, 204/299 R, 204/182.8
• US Class Current: 204/612
• CPC Class Codes: G01N 27/44721 by optical means

Abstract

An apparatus for determining a base sequence comprising an assembly for making an exciting beam incident on a slab of electrophoretic gel in a direction normal to the plane thereof, the gel containing nucleic acid fragments labeled with a labeling pigment and developed or being developed therein by electrophoresis, and a light receiving unit for receiving at an end face of the gel the luminescent emitted by the labeling pigment on the fragments developed in the gel.

Claims

1. An apparatus for determining a base sequence comprising an assembly for making an exciting beam incident on a surface of a slab of electrophoretic gel in a direction normal to said surface, the gel containing nucleic acid fragments labeled with a labeling pigment and developed or being developed therein by electrophoresis;

means for concentrating said exciting beam on said gel;

means for scanning said surface of said gel with said exciting beam; and

a light receiving unit for receiving at an end face of said gel the luminescence emitted by said labeling pigment on the fragments developed in said gel.

2. An apparatus as defined in claim 1 wherein the light receiving unit comprises a bundle of optical fibers having one end face in the form of a rectangle of small width and shaped in conformity with the shape of said end face of the gel and the other end face opposed to the sensitive surface of a photoelectric device.

3. An apparatus as defined in claim 1 wherein the light receiving unit comprises a shaped optical product having one end face in the form of a rectangle of small width and shaped in conformity with the shape of said end face of the gel and the other end face opposed to the sensitive surface of a photoelectric device.

4. An apparatus as defined in claim 1 which comprises means for correcting the intensity of the received luminescence at the position of incidence of the exciting beam.

5. An apparatus as defined in claim 1 wherein the gel is covered with a reflecting mirror over at least one of the end faces, front surface and rear surface thereof other than said end face of the gel and the front surface portion thereof to be exposed to the exciting beam.

6. An apparatus for determining a base sequence comprising an assembly for making an exciting beam incident on a slab of electrophoretic gel in a direction normal to the plane thereof, the gel containing nucleic acid fragments labeled with a labeling pigment and developed or being developed therein by electrophoresis, and a light receiving unit for receiving at an end face of the gel the luminescence emitted by the labeling pigment on the fragments developed in the gel, wherein the incidence assembly comprises an excitation light source, a condenser lens for concentrating an exciting beam from the light source, and means for making the concentrated exciting beam incident on the gel having the fragments developed therein by electrophoresis in a direction normal to the plane of the slab of gel and for scanning the surface of the gel with the beam in the direction of electrophoresis and in a direction perpendicular thereto.

7. An apparatus for determining a base sequence comprising an assembly for making an exciting beam incident on a slab of electrophoretic gel in a direction normal to the plane thereof, the gel containing nucleic acid fragments labeled with a labeling pigment and developed or being developed therein by electrophoresis, and a light receiving unit for receiving at an end face of the gel the luminescence emitted by the labeling pigment on the fragments developed in the gel, wherein the incidence assembly comprises an excitation light source, and means for concentrating an exciting beam from the light source, making the concentrated exciting beam incident on the gel having the fragments being developed therein by electrophoresis in a direction normal to the plane of the slab of gel and scanning the surface of the gel with the beam on a straight line perpendicular to the direction of electrophoresis.

8. An apparatus for determining a base sequence, comprising an assembly for making an exciting beam of laser light incident on a surface of a slab of electrophoretic gel in a direction normal to the plane of said surface, said gel containing nucleic acid fragments labeled with a labeling pigment and developed or being developed therein by electrophoresis;

means for scanning the surface of said gel with said exciting beam; and

a light receiving unit for receiving, at an end face of said gel, luminescence emitted by said labeling pigment on said fragments developed in said gel.

9. The apparatus as defined in claim 8, further comprising means for condensing said exciting beam on said gel.

10. The apparatus as defined by claim 8, wherein said exciting beam of laser light is an argon laser beam.