Method of producing an artifical skin

• US 4,883,487 A
• Filed: 04/06/1987
• Issued: 11/28/1989
• Est. Priority Date: 04/19/1986
• Status: Expired due to Fees

• Current Assignee: Koken Limited
• Original Assignee: Koken Limited
• Inventors: Taira, Toshio, Yoshizato, Katsutoshi, Miyata, Teruo
• Primary Examiner(s): Apley, Richard J.
• Assistant Examiner(s): Isabella, David J.
• Application Number:
  US07/034,958
• Time in Patent Office: 967 Days
• Field of Search: 128/155, 623/1, 623/11, 623/15, 623/16
• US Class Current: 128/898
• CPC Class Codes: A61F 2/10 Hair or skin implants
• CPC Class Codes: A61F 2/105 Skin implants, e.g. artificial skin
• CPC Class Codes: A61F 2310/00365 Proteins; Polypeptides; Degradation products thereof
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/36 containing ingredients of undetermined constitution or reaction products thereof , e.g. transplant tissue, natural bone, extracellular matrix isolated natural compounds, e.g. collagen A61L27/24
• CPC Class Codes: A61L 27/60 Materials for use in artificial skin
• CPC Class Codes: Y10S 623/901 Method of manufacturing prosthetic device

Abstract

An artifical skin comprising an insoluble atelocollagen sheet and an epidermal cell layer cultured only on one surface of the sheet, and a method of producing the same are disclosed, which method includes the steps of: forming an insoluble atelocollagen sheet; inoculating epidermal cells only on one surface of the atelocollagen sheet; and suspending the atelocollagen sheet in a liquid culture medium to culture the epidermal cells, thereby forming an epidermal cell layer.

Claims

1. A method of producing an artificial skin comprising:

(a) pouring an acidic atelocollagen solution into a mold to a depth of 1 to 5 mm;

(b) air-drying said poured atelocollagen solution to form a dry sheet;

(c) cross-linking said dry sheet by ultraviolet irradiation for a time sufficient to form an insoluble atelocollagen sheet;

(d) inoculating epidermal cells on one surface of said atelocollagen sheet; and

(e) suspending the inoculated atelocollagen sheet in a liquid culture medium to culture said epidermal cells, thereby forming an epidermal cell layer.

2. A method according to claim 1, further comprising culturing fibroblasts on at least one surface of said insoluble altelocollagen sheet prior to inoculating with epidermal cells.

3. A method according top claim 1, wherein the concentration of atelocollagen in the poured solution is 0.3 to 1.0%.