Method of reendothelializing vascular linings

• US 4,883,755 A
• Filed: 10/28/1987
• Issued: 11/28/1989
• Est. Priority Date: 10/28/1987
• Status: Expired due to Term

• Current Assignee: Thomas Jefferson University (Main Line Health System)
• Original Assignee: Thomas Jefferson University (Main Line Health System)
• Inventors: Carabasi, R. Anthony, Williams, Stuart K., Jarrell, Bruce E.
• Primary Examiner(s): Warren, Charles F.
• Assistant Examiner(s): HOLLRAH, GLENNON
• Application Number:
  US07/114,242
• Time in Patent Office: 762 Days
• Field of Search: 435/240.2, 435/240.25, 435/240.1, 435/1, 435/2, 424/77, 424/407, 514/2, 514/802, 604/4
• US Class Current: 424/93.7
• CPC Class Codes: A61F 2/06 Blood vessels
• CPC Class Codes: A61F 2/062 Apparatus for the production of blood vessels made from natural tissue or with layers of living cells prostheses made from natural tissue or living cells A61L27/507
• CPC Class Codes: A61K 35/14 Blood; Artificial blood perfluorocarbons A61K31/02; umbilical cord blood A61K35/51; haemoglobin A61K38/42
• CPC Class Codes: A61K 35/16 Blood plasma; Blood serum umbilical cord blood A61K35/51
• CPC Class Codes: A61K 35/44 Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
• CPC Class Codes: A61L 27/36 containing ingredients of undetermined constitution or reaction products thereof , e.g. transplant tissue, natural bone, extracellular matrix isolated natural compounds, e.g. collagen A61L27/24
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062
• CPC Class Codes: A61P 9/14 Vasoprotectives; Antihaemorrhoidals; Drugs for varicose therapy; Capillary stabilisers
• CPC Class Codes: C12N 5/069 Vascular Endothelial cells

Abstract

A method for treating the vascular passage of a patient, damaged by procedures such as an endarterectomy which denude portions of the vascular passages of their endothelial cell linings, is disclosed. In this method, endothelial cells are isolated from the patient'"'"'s own microvessels, the flow of blood through the patient'"'"'s damaged vascular passage is interrupted, the endothelial cells isolated from the patient'"'"'s microvessels are applied to the surface of the denuded portion of the patient'"'"'s vascular passage in a density sufficient to provide covereage of at least about 50% of said denuded portion, and interruption of blood flow through the vascular passage is maintained for a period of time sufficient to allow the sodded cells to form an attachment to the vascular lining sufficient to withstand the shear created by resumed blood flow through the vascular passage.

Claims

1. A method of reendothelializing the vascular passage of a patient, the lining of which has been substantially denuded of endothelial cells, comprising

(a) interrupting the flow of blood through said vascular passage,

(b) applying to said denuded vascular lining uncultured endothelial cells retrieved from the microvessels of said patient in a density sufficient to provide coverage of at least about 50% of said denuded portion;

and

(c) maintaining the interruption of blood flow through said vascular passage for a period of time sufficient to allow 5 said applied cells to form an attachment to said vascular lining sufficient to withstand the shear created by resumed blood flow through said vascular passage.

2. The method of claim 1 where said endothelial cells are applied in a density of at least about 1×

10⁵ cells per cm² of seeded surface.

3. The method of claim 1 where said endothelial cells are applied in a density sufficient to form a confluent layer of said cells on said vascular lining.

4. The method of claim 1 where said endothelial cells are applied to said vascular passage in a suspension of blood or plasma.

5. The method of claim 2 where said endothelial cells are applied to said vascular passage in a suspension of blood or plasma.

6. The method of claim 3 where said endothelial cells are applied to said vascular passage in a suspension of blood or plasma.

7. The method of claim 1 where said endothelial cells are applied to said vascular passage in a suspension of a non-gel-forming buffer solution.

8. The method of claim 2 where said endothelial cells are applied to said vascular passage in a suspension of a non-gel-forming buffer solution.

9. The method of claim 3 where said endothelial cells are applied to said vascular passage in a suspension of a non-gel-forming buffer solution.

10. The method of claim 1 where said endothelial cells are derived from the fat tissues of the patient.

11. The method of claim 4 where said endothelial cells are derived from the fat tissues of the patient.

12. The method of claim 7 where said endothelial cells are derived from the fat tissues of the patient.

13. The method of claim 1 where said endothelial cells are retrieved from said patient and applied to said patient'"'"'s vascular lining during the sam operative procedure.