Sample processor card for centrifuge

• US 4,883,763 A
• Filed: 09/30/1988
• Issued: 11/28/1989
• Est. Priority Date: 05/03/1984
• Status: Expired due to Term

• Current Assignee: Abbott Laboratories Incorporated
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Holen, James T., Kazlauskas, Vidas P.
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US07/253,370
• Time in Patent Office: 424 Days
• Field of Search: 422/58, 422/61, 422/72, 422/102, 422/104, 436/45, 436/165, 436/177, 436/180, 356/426, 356/422, 356/246, 250/576
• US Class Current: 436/45
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B04B 5/0407 for liquids contained in receptacles B04B5/0442 takes precedence
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A sample processor card for use with a centrifuge in which the direction of centrifugal force can be altered at will, wherein the card includes a supply of chemical reagent and inlet means for supplying a chemical sample to the card. The sample is advanced under centrifugal force through capillary means to sample measuring means, and the measured sample is then mixed with reagent to permit a chemical test to be carried out on the reagent, all under centrifugal force.

Claims

1. A sample processor card for carrying out chemical tests under centrifugal force, said centrifugal force being applied in at least two directions relative to said card by the orientation of the card on a centrifuge rotor, said card comprising:

means defining an outer enclosure having a plurality of walls defining therein;

(a) inlet means for supplying a sample to the card, sample measuring chamber means communicating with said inlet means to measure a volume of sample, and a sample overflow means communicating with the inlet means and the sample measuring chamber means, said sample measuring chamber means and said sample overflow means being disposed such that centrifugal force acting in a first direction relative to said card moves sample into said sample measuring chamber means, with excess sample moving into said sample overflow means;

(b) reagent containing means for supplying at least one reagent;

(c) mixing chamber means communicating with said sample measuring chamber means and said reagent containing means and disposed such that centrifugal force acting on the card in a second direction relative to the card admits said measured volume of sample and said reagent into said mixing chamber means; and

(d) cuvette means communicating with the mixing chamber means;

wherein said sample measuring chamber means, said reagent containing means, said mixing chamber means and said cuvette means are arranged in said enclosure such that movement of sample or reagent among said sample measuring chamber means, said reagent containing means, said mixing chamber means and said cuvette means can be accomplished solely by changing the orientation of the processor card relative to a centrifuge rotor without substantially lowering the magnitude of centrifugal force.

2. The card as defined in claim 1 wherein the enclosure further defines sample separating chamber means disposed intermediate and in communication with the inlet means and the sample measuring chamber means and adapted to separate, under centrifugal force in one of said first and second directions, constituents of the sample.

3. The card as defined in claim 1 wherein the card further includes means for dispensing reagent solely in response to centrifugal force applied to the card.

4. The card as defined in claim 3 wherein the means for dispensing reagent includes means for dispensing reagent and means for dispensing diluent for the reagent.

5. The card as defined in claim 3 wherein the means for dispensing reagent includes reagent chamber means adapted to contain the reagent and strippable sealing means closing the reagent chamber means whereby the sealing means is stripped from the reagent chamber means in response to the application of centrifugal force applied to the card to release reagent from the chamber means.

6. The card as defined in claim 5 which includes diluent chamber means positioned adjacent to the reagent chamber means, the sealing means closing the reagent chamber means and the diluent chamber means whereby the application of centrifugal force releases reagent and diluent.

7. The card as defined in claim 6 wherein the reagent chamber means is positioned forward of the diluent chamber means whereby the reagent is released prior to the release of diluent in response to the application of centrifugal force.

8. The card as defined in claim 5 which includes means for securing to the card the sealing means whereby the application of centrifugal force to the card displaces the reagent chamber means in the direction of the centrifugal force to strip the sealing means from the reagent chamber means to thereby release the reagent.

9. The card as defined in claim 3 wherein the means for dispensing reagent comprises a reagent chamber means having a portion thereof adapted to be punctured by an impinging means in response to centrifugal force applied to said card.

10. The card of claim 9 which includes a diluent chamber means having a portion thereof adapted to be punctured by an impinging means in response to centrifugal forces applied to said card.

11. The card as defined in claim 1 wherein said enclosure further defines a plurality of channel and chamber means disposed intermediate and communicating with said reagent containing means whereby the flow and measurement of said reagent and/or diluent can be adjusted.

12. The card as defined in claim 1 wherein the enclosure defines a plurality of baffles defining restricted openings positioned between the mixing chamber means and the cuvette means so that the reagent and sample pass through the restricted openings while flowing, in response to centrifugal force applied to the card in either direction, between the mixing chamber means and the cuvette means to facilitate intimate mixing between the reagent and the sample.

13. The card as defined in claim 1 wherein the enclosure defines a key opening extending into the card and adapted to receive a key to insure that the card is properly registered in a centrifuge.

14. The card as defined in claim 1 wherein the means defining the outer enclosure further includes substantially parallel upper and lower surfaces and side walls having a double bevel with an apex between said upper and lower surfaces, said apex adapted for securing the card to a centrifuge rotor.

15. A method for carrying out chemical tests under centrifugal force on a centrifuge rotor, comprising the steps of:

(a) supplying a sample to be tested to a sample processor card having

(1) inlet means for supplying a sample to the card, sample measuring chamber means communicating with said inlet means to measure a volume of sample, and a sample overflow means communicating with the inlet means and the sample measuring chamber means, said sample measuring chamber means and said sample overflow means being disposed such that centrifugal force acting in a first direction relative to said card moves sample into said sample measuring chamber means, with excess sample moving into said sample overflow means;

(2) reagent containing means for supplying at least one reagent;

(3) mixing chamber means communicating with said sample measuring chamber means and said reagent containing means and disposed such that centrifugal force acting on the card in a second direction relative to the card admits said measured volume of sample and said reagent into said mixing chamber means; and

(4) cuvette means communicating with the mixing chamber means;

(b) subjecting the card to a substantially uniform and continuous centrifugal force acting in a first direction relative to the card to move sample into said sample measuring chamber means, with excess sample moving into said sample overflow means;

(c) reorienting the card relative to a centrifuge rotor to change the effective direction of centrifugal force acting thereon to a second direction, thereby to contact the sample with reagent and effect a chemical reaction therebetween, said contact being accomplished solely by changing the relative direction of centrifugal force and without substantially lowering the magnitude of centrifugal force; and

(d) measuring the reaction product of the sample and reagent.

16. A method as defined in claim 15 which includes the step of centrifugally separating the sample constituents before contacting the sample with reagent.

17. A method as defined in claim 15 which includes the step of releasing reagent from a closed container in response to centrifugal force.

18. A method as defined in claim 15 which includes the step of mixing reagent with the sample.

19. A method as defined in claim 18 wherein the sample and reagent are mixed by passing through at least one restricted opening under the effect of centrifugal force.

20. A method as defined in claim 19 wherein the reagent and sample are passed through the restricted opening by centrifugal force acting in a first direction and the card is rotated to change the direction of the centrifugal force to pass the reagent and sample again through the restricted opening in the opposite direction.

21. A method as defined in claim 15 which includes the step of measuring a substantially precise amount of reagent and/or diluent for contacting the sample and retaining excess reagent and/or diluent.