Spectroscopic method and apparatus for measuring sugar concentrations
First Claim
1. A spectroscopic method for measuring concentrations of sugars in samples, which comprisesa first step wherein light intensity of a light source in an interferometer is set at a first prescribed value and outcoming light beams from said interferometer are emitted into an integrating sphere to irradiate a sample placed at a sample aperture of said integrating sphere,a second step wherein diffusely reflected light beams from said sample are detected by a photo detector,a third step wherein absorption spectrum of the sugar under measurement at a first depth in said sample is determined by processing electrical signals from said photo detector by a computer,a fourth step wherein the light intensity of the light source in said interferometer is set at a second prescribed value and absorption spectrum of the sugar under measurement at a second depth in said sample is determined in the same manner as in said first, second and third steps, anda fifth step wherein absorption spectrum of the sugar under measurement at a deeper region between said first and second depths in the sample is determined on the basis of the difference between the respective absorption spectrum determined in said third and fourth steps so that the concentration of the sugar under measurement in said deeper region in said sample can be determined on the basis of said absorption spectrum at said deeper region,the wavelengths for a use for said absorption spectrum being selected from a wavelength band of from 950 to 1,150 nm, from 1,150 to 1,300 nm, or from 1,300 to 1,450 nm.
1 Assignment
0 Petitions
Accused Products
Abstract
This disclosure relates to a method for measuring the concentrations of sugars in liquids by the use of near infrared as well as an apparatus for practicing said method.
The method comprises a step wherein the absorption spectrum of the sugar under investigation existing at a first depth relatively close to the surface in a sample is computed by using relatively weak power of near infrared, a step wherein the absorption spectrum of the sugar under investigation existing at a relatively deep second depth in the sample is computed by using relatively strong power of infrared, and a step wherein the concentration of the sugar under investigation in the sample is determined by computing the absorption spectrum of the sugar at a different depth between said first and second depths on the basis of the results of measurement in said first and second steps. The wavelengths for the use for said absorption spectrum are selected from anyone of wavelength bands of 950-1,150 nm, 1,150-1,300 nm, and 1,300-1,450 nm.
Since, according to this method, wavelengths which are shorter and closer to the visible region than in the prior art are used, the method enables the measuring light beams to penetrate deeper in a living tissue and makes it possible to measure the concentrations of sugars existing in a deeper region non-invasively.
-
Citations
5 Claims
-
1. A spectroscopic method for measuring concentrations of sugars in samples, which comprises
a first step wherein light intensity of a light source in an interferometer is set at a first prescribed value and outcoming light beams from said interferometer are emitted into an integrating sphere to irradiate a sample placed at a sample aperture of said integrating sphere, a second step wherein diffusely reflected light beams from said sample are detected by a photo detector, a third step wherein absorption spectrum of the sugar under measurement at a first depth in said sample is determined by processing electrical signals from said photo detector by a computer, a fourth step wherein the light intensity of the light source in said interferometer is set at a second prescribed value and absorption spectrum of the sugar under measurement at a second depth in said sample is determined in the same manner as in said first, second and third steps, and a fifth step wherein absorption spectrum of the sugar under measurement at a deeper region between said first and second depths in the sample is determined on the basis of the difference between the respective absorption spectrum determined in said third and fourth steps so that the concentration of the sugar under measurement in said deeper region in said sample can be determined on the basis of said absorption spectrum at said deeper region, the wavelengths for a use for said absorption spectrum being selected from a wavelength band of from 950 to 1,150 nm, from 1,150 to 1,300 nm, or from 1,300 to 1,450 nm.
-
5. A spectrophotometric apparatus for measuring concentrations of sugars in samples, which comprises
an interferometer for emitting superposed light beams, a controller for adjusting light intensity of a light source which is placed in said interferometer, an integrating sphere for collecting diffusely reflected light beams from a sample placed at said integrating sphere, a photo detector for detecting said collected light and converting it into an electric signal, a processing means for processing electric signal from said photo detector so as to compute absorption spectrum of the sugar in said sample to be determined and for determining the concentration of the sugar under measurement on the basis of said absorption spectrum, and a means for selecting optimum wavelengths for a use for said absorption spectrum from a wavelength band of from 950 to 1,150 nm, from 1,150 to 1,300 nm, or from 1,300 to 1,450 nm, said processing means including a means whereby, on the basis of the difference between the absorption spectrum of the sugar under measurement at a first depth in said sample which is computed for light intensity of said light source in said interferometer set at a first prescribed value and absorption spectrum of said sugar at a second depth which is computed for light intensity of said source set at a second prescribed value, the absorption spectrum of the sugar under measurement at a deeper region between said first and second depths in said sample is computed and including furthermore a means whereby, on the basis of said absorption spectrum at said deeper region, the concentration of the sugar under measurement in said deeper region in the sample is computed.
Specification