Enzyme immobilization and bioaffinity separations with perfluorocarbon polymer-based supports
First Claim
1. A solid support containing an attached ligand or binder for the ligand consisting essentially of:
- (A) a chemically inert, water immiscible solid perfluorocarbon polymer carrier having low nonspecific binding to a ligand or binder for the ligand;
(B) a perfluorocarbon-substituted ligand or binder for the ligand securely but reversibly attached to the surface of said carrier; and
(C) a nonionic fluorosurfactant coating on said carrier.
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Accused Products
Abstract
A bioaffinity separation method is provided along with a solid affinity support utilized in that method. Additionally, immobilized enzyme systems are provided for use as enzyme electrode systems. The support is based on an inert perfluorocarbon polymer carrier with ligands or binders attached to its surface. The ligand, binder or enzyme is preferably modified by attaching a perfluorocarbon anchor group, and the modified ligand, binder or enzyme is attached to the carrier through the anchor group. Methods for preparing such supports and their use in capturing target molecules from samples and in analytical applications are also provided.
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Citations
23 Claims
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1. A solid support containing an attached ligand or binder for the ligand consisting essentially of:
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(A) a chemically inert, water immiscible solid perfluorocarbon polymer carrier having low nonspecific binding to a ligand or binder for the ligand; (B) a perfluorocarbon-substituted ligand or binder for the ligand securely but reversibly attached to the surface of said carrier; and (C) a nonionic fluorosurfactant coating on said carrier. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A bioaffinity separation process comprising the steps of:
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(A) forming a solid affinity support by (a) attaching a perfluorocarbon-substituted ligand or binder for the ligand to the surface of a solid perfluorocarbon polymer carrier; and (b) contacting the product of step (a) with a nonionic fluorosurfactant to form a solid affinity support; and (B) capturing a binder or ligand, complementary to the ligand or binder attached to the carrier from a mixture using said solid affinity support. - View Dependent Claims (10, 11, 12, 13, 14, 15)
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16. An immobilized enzyme system consisting essentially of:
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(A) a chemically inert, water immiscible solid perfluorocarbon polymer carrier having low nonspecific binding to a ligand or binder for the ligand; (B) a perfluorocarbon-substituted enzyme attached securely but reversibly to the surface of said carrier; and (C) a nonionic fluorosurfactant coating on said carrier.
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17. A process of preparing a solid support containing an attached ligand or binder for the ligand comprising the steps of:
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(A) modifying a ligand or binder for the ligand by attaching a perfluorocarbon anchor group to said ligand or binder for the ligand; (B) contacting a solid perfluorocarbon polymer with the perfluorocarbon-substituted ligand or binder for the ligand to attach the ligand or binder to the polymer through said anchor group; and (C) contacting the product formed in step (B) with a nonionic fluorosurfactant. - View Dependent Claims (18, 19, 20, 21, 22, 23)
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Specification