Protein label and drug delivery system
First Claim
1. A method of conjugating a protein with a bridging compound having the formula ##STR8## wherein X and Y comprise separate functional groups reactive with sulfhydryl groups formed by reduction of disulfide bonds of a protein molecule and A comprises a drug, dye or label portion which is unreactive with sulfhydryl groups, said method comprising the steps of:
- (a) reducing the disulfide bonds of said protein;
(b) reacting the bridging compound across the reduced disulfide bonds of said protein; and
(c) oxidizing or alkylating the reduced disulfide bonds remaining after step (b).
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Abstract
A bridging molecule carrying a drug, or a label such as a fluorophore, which adds across disulfide bonds of molecules, particularly proteins, and methods of manfacturing and using the bridging molecules, are disclosed. The bridging molecule is reactive with sulfhydryl groups formed by the reduction of disulfide bonds of the protein. The functional groups of the bridging molecules are typically --SH groups.
22 Citations
12 Claims
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1. A method of conjugating a protein with a bridging compound having the formula ##STR8## wherein X and Y comprise separate functional groups reactive with sulfhydryl groups formed by reduction of disulfide bonds of a protein molecule and A comprises a drug, dye or label portion which is unreactive with sulfhydryl groups, said method comprising the steps of:
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(a) reducing the disulfide bonds of said protein; (b) reacting the bridging compound across the reduced disulfide bonds of said protein; and (c) oxidizing or alkylating the reduced disulfide bonds remaining after step (b). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification