High resolution monitoring device

• US 4,891,185 A
• Filed: 01/22/1988
• Issued: 01/02/1990
• Est. Priority Date: 01/22/1988
• Status: Expired due to Term

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Goldin, Stanley M.
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Griffith, Rebekah A.
• Application Number:
  US07/146,818
• Time in Patent Office: 711 Days
• Field of Search: 422/67, 422/69, 422/72, 422/81, 422/82, 422/99, 422/101, 422/103, 436/44, 436/50, 436/57, 436/162, 436/178, 436/180, 436/527, 210/658, 210/659, 73/863.01, 73/863.23, 73/863.71, 73/863.73, 73/864.83, 137/624.12, 137/625.4, 137/628, 222/644
• US Class Current: 422/69
• CPC Class Codes: G01N 13/04 Investigating osmotic effects
• CPC Class Codes: G01N 2001/1037 from an enclosure (hazardous waste, radioactive)
• CPC Class Codes: Y10T 436/115831 Condition or time responsive
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A device for monitoring the release of a substance from a substrate, e.g., the release of ions through membranes, are disclosed.

Claims

1. A device for monitoring the release of a substance from a substrate, said device comprising

a sample chamber adapted to be connected to a solution source and through which a solution supplied by said solution source can flow, said sample chamber comprising a retainer for holding a substance immobilized by a substrate while said supplied solution flows in contact with said immobilized substance and removes any said substance released from said substrate;

a solution collector positioned to receive the solution flowing from said sample chamber and including a substance absorbant having an exposed face and means for supporting said substance absorbant,

said sample chamber including means for directing a stream of said solution from said sample chamber into contact with a portion only of said exposed face of said substance absorbant; and

means for continuously moving the location of said contact of said stream with respect to said exposed face of said substance absorbant.

2. The device of claim 1, said sample chamber comprising flow paths for said solution to flow from said solution source to said solution collector, a first said flow path passing through said retainer, a second relatively unobstructed flow path not passing through said retainer but merging with said first flow path after said first flow path passes through said retainer.

3. The device of claim 1 wherein said means for continuously moving comprises a rotating disc holding said substance absorbant.

4. The device of claim 1 wherein said substrate comprises a structure surrounded by a biological membrane and wherein said substance is released through said biological membrane.

5. The device of claim 1 wherein said retainer comprises a depth filter.

6. The device of claim 5 wherein said depth filter comprises a glass fiber filter.

7. The device for monitoring the release of a substance from a substrate, said device comprising

a sample chamber adapted to be connected to a solution source and through which a solution supplied by said solution source can flow,

said sample chamber comprising a retainer for holding a substance immobilized by a substrate while said supplied solution flows over said immobilized substance removing any said substance released by said substrate, and flow paths for said solution to flow from said solution source to a solution collector, a first said flow path passing through said retainer, a second relatively unobstructed flow path not passing through said retainer but merging with said first flow path after said first flow path passes through said retainer;

and

said solution collector positioned to receive the solution from the merged flow paths.

8. The device of claim 7 wherein said substrate comprises a structure surrounded by a biological membrane and wherein said substance is released through said biological membrane.

9. A device for monitoring the release of a substance from a substrate, said device comprising

a solution source;

a sample chamber adapted to be connected to said solution source and through which a solution supplied by said solution source can flow, said sample chamber comprising

a retainer for holding a substance immobilized by a substrate while said supplied solution flows in contact with said immobilized substance and removes any said substance released from said substrate, and

a plurality of valves corresponding to a plurality of solutions, said valves being responsive to control signals to deliver the solutions corresponding thereto to said sample chamber,

said solution source including means for applying the control signals to said valves, said control signal applying means being programmable to selectively control the timing and duration of the delivery of solution by each valve; and

a solution collector positioned to receive the solution that has flowed over said retainer in said sample chamber.

10. The device of claim 9 wherein said control signal applying means comprises

(a) means for generating a valve activating signal,

(b) means for generating a valve enable signal to enable said valve activating signal to be coupled to said plurality of valves, and

(c) means for switchably coupling said enabled valve activating signal to said plurality of valves to selectively cause said valves to deliver their respective solutions to said sample chamber, said coupling means being programmable to vary the timing and duration of the activation of each said valve.

11. The device of claim 10, said solution collector being positioned to receive the solution flowing from said sample chamber and including a substance absorbant having an exposed face and means for supporting said substance absorbant, said sample chamber including means for directing a stream of said solution from said sample chamber into contact with a portion only of said substance absorbant;

and said device further comprising means for continuously moving the location of said contact of said stream with respect to said exposed face of said substance absorbant.

12. The device of claim 11 wherein said means for continuously moving comprises a rotating disc holding said substance absorbant, and wherein said valve enable signal generating means is synchronized with the rotation of said rotating disc.

13. The device of claim 12 wherein said means for switchably coupling said enabled valve activating signal to said plurality of valves is also synchronized with said rotation of said rotating disc.

14. The device of claim 11 wherein

(a) said means for continuously moving comprises a rotating disc holding said substance absorbant

(b) the delivery of said stream of solution forms a pattern on said substance absorbant;

(c) said valve enable signal generating means is synchronized with the rotation of said rotating disc to produce said valve enable signal for a duration equal to the time required to generate said spiral pattern; and

(d) said means for switchably coupling the valve activating signal to said plurality of valves comprises;

means for producing an output signal having a first state corresponding to the activation of a first one of said plurality of valves and a second state corresponding to the activation of a second one of said plurality of valves, the relative timing and durations of the first and second states being programmable; and

, means for coupling said valve activating signal to said first valve in response to said first state of said output signal and coupling said valve activating signal to said second valve in response to said second state of said output signal.

15. The device of claim 14 wherein said output signal producing means is programmed to produce a series of pulses of said second state thereof during the formation of said pattern.

16. The device of claim 14 wherein said output signal producing means is also synchronized with the rotation of said rotating disc and is programmable to produce a pulse of the second state thereof at a predetermined time within the duration of said valve enable signal.

17. The device of claim 16 wherein said output signal producing means is programmed to produce a pulse of said second state thereof during each revolution of said rotating disc, each said pulse being synchronized with the rotation of said rotating disc.

18. The device of claim 16 wherein the pulses produced during each revolution of said rotation disc are synchronized to be aligned on said pattern.