Protection of proteins and the like
First Claim
1. A method of protecting proteins or other biological macromolecules against denaturation during drying, comprising subjecting an aqueous system containing the protein or other biological macromolecule to drying at a temperature above freezing in the presence of trehalose in an amount between about 0.05 and 20 weight percent based on the total weight of said aqueous system.
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Accused Products
Abstract
Sensitive proteins and other macromolecules, such as enzymes, antibodies, antigens, serum complement, fluorescent proteins, vaccine components, polysaccharides such as agarose etc, can be preserved by drying at ambient temperature and at atmospheric pressure in the presence of trehalose. A porous matrix impregnated with trehalose is provided as a receiver for a blood or other liquid sample to be dried.
357 Citations
12 Claims
- 1. A method of protecting proteins or other biological macromolecules against denaturation during drying, comprising subjecting an aqueous system containing the protein or other biological macromolecule to drying at a temperature above freezing in the presence of trehalose in an amount between about 0.05 and 20 weight percent based on the total weight of said aqueous system.
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5. A product dried at a temperature above freezing containing trehalose and a protein or other biological macromolecule in a weight ratio of at least 1.4:
- 1 respectively.
- View Dependent Claims (6)
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7. A plate having adhered thereto one or more antibodies dried at a temperature above freezing in the presence of trehalose in an amount at least about 1.4 times greater by weight than the amount of said one or more antibodies.
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11. A method for protecting a protein or other biological macromolecule against denaturation during drying comprising subjecting an aqueous system containing said protein or other biological macromolecule to drying at a temperature above freezing in the presence of trehalose in an amount of at least about 1.4 time greater by weight than the amount of said protein or other biological macromolecule.
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12. A method for protecting a protein or other biological macromolecule against denaturation during drying comprising subjecting an aqueous system containing said protein or other biological macromolecule to drying at a temperature above freezing in the presence of trehalose in an amount of at least about 0.05 weight percent based on the total weight of said aqueous system.
Specification