Enzyme electrode

• US 4,894,137 A
• Filed: 09/14/1987
• Issued: 01/16/1990
• Est. Priority Date: 09/12/1986
• Status: Expired due to Term

• Current Assignee: Omron Healthcare Company Limited (Omron Corporation)
• Original Assignee: Omron Tateisi Electronics Co. (Omron Corporation)
• Inventors: Arai, Masato, Nakajima, Satoshi, Takizawa, Koichi
• Primary Examiner(s): Kaplan, G. L.
• Application Number:
  US07/095,977
• Time in Patent Office: 855 Days
• Field of Search: 204/403, 204/1 E, 435/291, 435/817, 435/288, 128/635
• US Class Current: 204/403.09
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

An enzyme electrode comprising: an insulative base plate; two or more electrodes formed on the surface of the insulative base plate and each having an exposed portion; an insulative protection film to insulate and protect the electrodes excluding at least those exposed portions; and an immobilized enzyme film to integratedly cover the exposed portions.

Claims

1. An enzyme electrode comprising:

an insulative base;

at least two electrodes including a working electrode and a reference electrode provided on the surface of said insulative base and each having an exposed portion and a connecting portion, the area of the exposed portion of the reference electrode being larger than that of the exposed portion of the working electrode;

an insulative protection film covering the portions excluding said exposed portions and connecting portions of said electrodes; and

an immobilized enzyme film which covers integrally at least the exposed portions of said working electrode.

2. An enzyme electrode according to claim 1, wherein windows are formed in said insulative protection film and parts of said electrodes are exposed from said windows, thereby forming said exposed portions and connecting portions.

3. An enzyme electrode according to claim 1, wherein said insulative protection film is made of a photosensitive resin.

5. An enzyme electrode according to claim 1, wherein said insulative base consists of a flexible insulative base plate, and said electrode comprises conductor patterns formed on the surface of said base plate and one or two or more plating layers laminated on said conductor patterns.

6. An enzyme electrode according to claim 1, wherein said insulative base consists of a flexible insulative base plate, said electrodes are made of conductive pastes formed on said base plate, another conductor film is laminated on the exposed portion of at least one of said electrodes, and the exposed portion onto which at least said conductor film is laminated is covered by the immobilized enzyme film.

7. An enzyme electrode according to claim 1, wherein said insulative base consists of a flexible insulative base plate, said electrodes are made of conductive pastes formed on said insulative base plate, a metal foil is adhered onto the exposed portion of at least one of the electrodes by a conductive adhesive agent, and the exposed portion on which at least said metal foil is adhered is covered by the immobilized enzyme film.

8. An enzyme electrode according to claim 1, wherein said insulative base is a flexible insulative base.

9. An enzyme electrode according to claim 1, wherein the area of the exposed portion of the reference electrode is at least 20 times as large as that of the exposed portion of the working electrode.

10. An enzyme electrode according to claim 1, wherein the working electrode, the reference electrode, the insulative protection film and the immobilized enzyme film are integrally fabricated on the insulative base.

4. An enzyme electrode according to claim 4, wherein the electrodes are respectively formed on at least two surfaces of said insulative base.

11. A combination enzyme electrode, comprising:

a pair of insulative bases;

a working electrode provided on the surface of one of said insulative bases and a reference electrode provided on the surface of the other of said insulative bases, each of said working electrode and said reference electrode having an exposed portion and a connecting portion, the area of the exposed portion of said reference electrode being larger than that of the exposed portion of said working electrode;

insulative protection films covering the portions of said insulative bases excluding said exposed portions and connecting portions of said electrodes; and

an immobilized enzyme film which integrally covers the exposed portion of said working electrode.

12. An enzyme electrode according to claim 11, wherein the area of the exposed portion of the reference electrode is at least 20 times as large as that of the exposed portion of the working electrode.

13. An enzyme electrode according to claim 11, wherein the working electrode, the reference electrode, the insulative protection films and the immobilized enzyme film are integrally fabricated on the respective insulative bases.

14. An enzyme electrode comprising:

an insulative base plate;

two or more electrodes provided on the surface of said insulative base plate and each having an exposed portion;

an insulative protection film insulating and protecting said electrodes except for said exposed portions; and

an immobilized enzyme film which integrally covers said exposed portion of at least one of said electrodes, said immobilized enzyme film comprising a first protective film formed on the portion including said exposed portion for preventing said electrode from sensing interfering materials, an enzyme film, and a second protective film for covering said enzyme film, said enzyme film being sandwiched between said first and second protective films.

15. An enzyme electrode according to claim 14, wherein said first and second protective films are acetyl cellulose films.

16. An enzyme electrode according to claim 14, wherein the area of the exposed portion of the reference electrode is at least 20 times as large as that of the exposed portion of the working electrode.

17. An enzyme electrode according to claim 14, wherein the working electrode, the reference electrode, the insulative protection film and the immobilized enzyme film are integrally fabricated on the insulative base.

18. An enzyme electrode comprising:

an insulative base plate;

an electrode provided on the surface of said insulative base plate and having an exposed portion;

an insulative protection film which insulates and protects said electrodes except for said exposed portion; and

an immobilized enzyme film which integrally covers said exposed portion of at least one of said electrodes, said immobilized enzyme film comprising a first protective film formed on the portion including said exposed portion for preventing said electrode from sensing interfering materials, an enzyme film, and a second protective film for covering said enzyme film, said enzyme film being sandwiched between said first and second protective films.

19. An enzyme electrode according to claim 18, wherein the area of the exposed portion of the reference electrode is at least 20 times as large as that of the exposed portion of the working electrode.

20. An enzyme electrode according to claim 18, wherein the working electrode, the reference electrode, the insulative protection film and the immobilized enzyme film are integrally fabricated on the insulative base.