Immobilized enzyme membrane for a semiconductor sensor

• US 4,894,339 A
• Filed: 12/17/1986
• Issued: 01/16/1990
• Est. Priority Date: 12/18/1985
• Status: Expired due to Fees

• Current Assignee: Mitsubishi Denki Kabushiki Kaisha (Mitsubishi Electric Corporation)
• Original Assignee: Seitaikinouriyou Kagakuhin Sinseizogijutsu Kenkyu Kumiai
• Inventors: Yamada, Satoshi, Shiono, Satoru, Hanazato, Yoshio, Nakako, Mamiko
• Primary Examiner(s): Naff, David M.
• Application Number:
  US06/942,797
• Time in Patent Office: 1,126 Days
• Field of Search: 435/174, 435/177, 435/180, 435/182, 435/817, 435/288, 204/403
• US Class Current: 435/182
• CPC Class Codes: C12N 11/082 obtained by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: C12N 11/091 Phenol resins; Amino resins
• CPC Class Codes: C12N 11/098 formed in the presence of the enzymes or microbial cells
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A membrane containing an immobilized enzyme for a semiconductor sensor is prepared containing a water soluble photosensitive resin including a high molecular weight polyvinyl pyrrolidone crosslinked to 2, 5-bis (4'"'"'-azide-2'"'"'-sulfobenzal) cyclopentanone sodium salt, and an enzyme. Glutaraldehyde and bovine serum albumin, polyamino acid or polyamino amino acid copolymer may also be present to provide chemical crosslinking. The enzyme may be glucose oxidase, urease or lipase. The membrane can be directly formed on ion-sensitive protions of a pH-ion sensitive field effect transistor to form a semiconductor sensor by coating an aqueous solution of the resin and enzyme on the ion-sensitive portion, drying and irradiating with light such as ultraviolet light to provide photo crosslinking.

Claims

1. A membrane containing an immobilized enzyme comprising:

a water soluble photosensitive resin including poly-vinyl pyrrolidone having a high molecular weight crosslinked to 2, 5-bis (4'"'"'-azide2'"'"'-sulfobenzal) cyclopentanone sodium salt; and

an enzyme.

2. A membrane as set forth in claim 1 wherein said enzyme is selected from the group consisting of glucose oxidase, urease and lipase.

3. A membrane as set forth in claim 1, further comprising;

glutaraldehyde; and

a material adapted to cooperate with glutaraldehyde to form chemical crosslinking.

4. A membrane as set forth in claim 3 wherein said enzyme is selected from the group consisting of glucose oxidase, urease and lipase.

5. A membrane as set forth in claim 3 wherein said material adapted to cooperate with glutaraldehyde to form chemical crosslinking is selected from the group consisting of bovine serum albumin, polyamino acid, and polyamino acid copolymer.

6. A membrane as set forth in claim 4 wherein said material adapted to cooperate with glutaraldehyde to form chemical crosslinking is selected from the group consisting of bovine serum albumin, polyamino acid, and polyamino acid copolymer.

7. A membrane as set forth in claim 5 wherein said polyamino acid is poly-L-lysine.

8. A membrane as set forth in claim 6 wherein said polyamino acid is poly-L-lysine.

9. A membrane as set forth in claim 5 wherein said polyamino acid copolymer is a copolymer of poly-L-lysine and poly-L-thyrosin.

10. A membrane as set forth in claim 6 wherein said polyamino acid copolymer is a copolymer of poly-L-lysine and poly-L-thyrosin.

11. A membrane as set forth in claim 1 wherein said polyvinyl pyrrolidone having a high molecular weight is 2-20 weight %;

and said 2, 5-bis (4'"'"'-azide-2'"'"'-sulfobenzal) cyclopentanone sodium salt is 0.5-1.5 weight %; and

said enzyme comprises glucose oxidase of 5.0-7.5 weight %;

said membrane further comprising bovine serum albumin of 5-10 weight %.

12. A method for producing a membrane containing an immobilized enzyme comprising the steps of:

coating on a base an aqueous solution which is composed of a water soluble photosensitive resin including polyvinyl pyrrolidone having a high molecular weight and 2,5-bis(4'"'"'-azide-2'"'"'-sulfobenzal) cyclopentanone sodium salt, and an enzyme;

drying the aqueous solution thus coated on said base so as to form a membrane containing an immobilized enzyme; and

irradiating said membrane containing an immobilized enzyme with ultraviolet light to provide photo-crosslinking between said polyvinyl pyrrolidone, said cyclopentanone sodium salt, and said enzyme.

13. A method for producing a membrane as set forth in claim 12 wherein said enzyme is selected from the group consisting of glucose oxidase, urease and lipase.

14. A method for producing a membrane as set forth in claim 12 wherein said aqueous solution further comprises;

glutaraldehyde; and

a material adapted to cooperate with glutaraldehyde to form chemical crosslinking.

15. A method for producing a membrane as set forth in claim 14 wherein said enzyme is selected from the group consisting of glucose oxidase, urease and lipase.

16. A method for producing a membrane as set forth in claim 14 wherein said material adapted to cooperate with glutaraldehyde to form chemical crosslinking is selected from the group consisting of bovine serum albumin, polyamino acid, and polyamino acid copolymer.

17. A method for producing a membrane as set forth in claim 15 wherein said material adapted to cooperate with glutaraldehyde to form chemical crosslinking is selected from the group consisting of bovine serum albumin, polyamino acid, and polyamino acid copolymer.

18. A method for producing a membrane as set forth in claim 16 wherein said polyamino acid is poly-L-lysine.

19. A method for producing a membrane as set forth in claim 17 wherein said polyamino acid is poly-L-lysine.

20. A method for producing a membrane as set forth in claim 16 wherein said polyamino acid copolymer is a copolymer of poly-L-lysine and poly-L-thyrosin.

21. A method for producing a membrane as set forth in claim 17 wherein said polyamino acid copolymer is a copolymer of poly-L-lysine and poly-L-thyrosin.

22. A method for producing a membrane as set forth in claim 12 wherein said polyvinyl pyrrolidone having a high molecular weight is 2-20 weight %;

and said 2, 5-bis (4'"'"'-azide-2'"'"'-sulfobenzal) cyclopentanone sodium salt is 0.5-1.5 weight %; and

said enzyme comprises glucose oxidase of 5.0-7.5 weight %;

said aqueous solution further comprising bovine serum albumin of 5-10 weight %.

23. A method for producing a membrane as set forth in claim 12 further comprising dipping said membrane after irradiation by said ultraviolet light into an aqueous solution of glutaraldehyde so as to form chemical crosslinking.

24. A method for producing a membrane as set forth in claim 23 further comprising washing the membrane containing an immobilized enzyme with a buffer solution after crosslinking.

25. A method for producing a membrane as set forth in claim 24 wherein said enzyme immobilized in said membrane is glucose oxidase.

26. A method for producing a membrane as set forth in claim 24 further comprising:

dipping said membrane containing an immobilized enzyme into an aqueous solution of glycine after washing with said buffer solution; and

again washing said membrane containing an immobilized enzyme with a buffer solution for removal of residual glutaraldehyde.

27. A method for producing a membrane as set forth in claim 26 wherein said enzyme immobilized in said membrane is glucose oxidase.

28. A sensor containing an enzyme comprising:

a first pH-ion sensitive field effect transistor having a membrane containing a first immobilized enzyme, said membrane being composed of a first water soluble photosensitive resin including polyvinyl pyrrolidone having a high molecular weight crosslinked to 2,5-bis(4'"'"'-azide-2'"'"'-sulfobenzal) cyclopentanone sodium salt, and said first enzyme;

a second pH-ion sensitive field effect transistor; and

a reference electrode.

29. A sensor containing an enzyme as set forth in claim 28 further comprising a third pH-ion sensitive field effect transistor having a membrane containing a second immobilized enzyme deposited thereon, said second membrane being composed of a second water soluble photosensitive resin including polyvinyl pyrrolidone having a high molecular weight of and 2,5-bis(4'"'"'-azide-2'"'"'-sulfobenzal) cyclopentanone sodium salt, and said second enzyme.

30. A sensor containing an enzyme as set forth in claim 28 wherein said first enzyme is selected from the group consisting of glucose oxidase, urease and lipase.

31. A sensor containing an enzyme as set forth in claim 29 wherein said second enzyme is selected from the group consisting of glucose oxidase, urease and lipase.

32. A sensor containing an enzyme as set forth in claim 28 further comprising;

glutaraldehyde; and

a material adapted to cooperate with glutaraldehyde to form chemical crosslinking.

33. A sensor containing an enzyme as set forth in claim 29 further comprising;

glutaraldehyde; and

a material adapted to cooperate with glutaraldehyde to form chemical crosslinking.

34. A sensor containing an enzyme as set forth in claim 31 wherein said first enzyme is selected from the group consisting of glucose oxidase, urease and lipase.

35. A sensor containing an enzyme as set forth in claim 32 wherein said second enzyme is selected from the group consisting of glucose oxidase, urease and lipase.

36. A sensor containing an enzyme as set forth in claim 32 wherein said material adapted to cooperate with glutaraldehyde to form chemical crosslinking in selected from the group consisting of bovine serum albumin, polyamino acid, and polyamino acid copolymer.

37. A sensor containing an enzyme as set forth in claim 33 wherein said material adapted to cooperate with glutaraldehyde to form chemical crosslinking is selected from the group consisting of bovine serum albumin, polyamino acid, and polyamino acid copolymer.

38. A sensor containing an enzyme as set forth in claim 34 wherein said material adapted to cooperate with glutaraldehyde to form chemical crosslinking is selected from the group consisting of bovine serum albumin, polyamino acid, and polyamino acid copolymer.

39. A sensor containing an enzyme as set forth in claim 35 wherein said material adapted to cooperate with glutaraldehyde to form chemical crosslinking is selected from the group consisting of bovine serum albumin, polyamino acid, and polyamino acid copolymer.

40. A sensor containing an enzyme as set forth in claim 36 wherein said polyamino acid is poly-L-lysine.

41. A sensor containing an enzyme as set forth in claim 37 wherein said polyamino acid is poly-L-lysine.

42. A sensor containing an enzyme as set forth in claim 38 wherein said polyamino acid is poly-L-lysine.

43. A sensor containing an enzyme as set forth in claim 39, wherein said polyamino acid is poly-L-lysine.

44. A sensor containing an enzyme as set forth in claim 36 wherein said polyamino acid copolymer is a copolymer of poly-L-lysine and poly-L-thyrosine.

45. A semiconductor enzyme sensor as set forth in claim 37 wherein said polyamino acid copolymer is a copolymer of poly-L-lysine and poly-L-thyrosine.

46. A sensor containing an enzyme as set forth in claim 38 wherein said polyamino acid copolymer is a copolymer of poly-L-lysine and poly-L-thyrosine.

47. A sensor containing an enzyme as set forth in claim 39 wherein said polyamino acid copolymer is a copolymer of poly-L-lysine and poly-L-thyrosine.

48. A sensor containing an enzyme as set forth in claim 28 wherein said polyvinyl pyrrolidone having a high molecular weight is 2-20 weight %;

and said 2,5-bis (4'"'"'-azide-2'"'"'-sulfobenzal) cyclopentanone sodium salt is 0.5-1.5 weight %; and

said enzyme comprises glucose oxidase of 5.0-7.5 weight %;

said membrane containing a first immobilized enzyme further comprising bovine serum albumin of 5-10 weight %.

49. A sensor containing an enzyme as set forth in claim 29 wherein said polyvinyl pyrrolidone having a high molecular weight is 2-20 weight %;

and said 2, 5-bis (4'"'"'-azide-2'"'"'-sulfobenzal) cyclopentanone sodium salt is 0.5-1.5 weight %; and

said enzyme comprises glucose oxidase of 5.0-7.5 weight %;

said membrane containing a second immobilized enzyme further comprising bovine serum albumin of 5-10 weight %.

50. A sensor containing an enzyme as set forth in claim 28 wherein said membrane containing a first immobilized enzyme is directly coated and hardened on said pH-ion sensitive field effect transistor.

51. A sensor containing an enzyme as set forth in claim 29 wherein said membrane containing a second immobilized enzyme is directly coated and hardened on said pH-ion sensitive field effect transistor.

52. A sensor containing an enzyme as set forth in claim 28 wherein said membrane containing a first immobilized enzyme is formed by coating on a pH-ion sensitive field effect transistor a first aqueous solution containing said first water soluble photosensitive resin, glucose oxidase, bovine serum albumin, and hardening said first aqueous solution thus coated by irradiation with ultraviolet light, said membrane containing a first immobilized enzyme being dipped into a glutaraldehyde solution to form chemical crosslinking for improved mechanical strength.

53. A sensor containing an enzyme as set forth in claim 29 wherein said membrane containing a second immobilized enzyme is formed by coating on a pH-ion sensitive field effect transistor a second aqueous solution containing said second water soluble photosensitive resin, glucose oxidase, bovine serum albumin, and hardening said second aqueous solution thus coated by irradiation with ultraviolet light, said membrane containing a second immobilized enzyme being dipped into a glutaraldehyde solution to form chemical crosslinking for improved mechanical strength.

54. A sensor containing an enzyme as set forth in claim 28 wherein said first pH-ion sensitive field effect transistor has an ionsensitive surface, and said membrane containing a first immobilized enzyme is formed through patterning on the ion-sensitive surface of said first pH-ion sensitive field effect transistor by means of photolithography.

55. A sensor containing an enzyme as set forth in claim 29 wherein said third pH-ion sensitive field effect transistor has an ion-sensitive surface, and said membrane containing a second immobilized enzyme is formed through patterning on the ion-sensitive surface of said third pH-ion sensitive field effect transistor by means of photolithography.

56. A sensor containing an enzyme as set forth in claim 28 further comprising one or more pH-ion sensitive field effect transistors each having a further membrane containing an immobilized enzyme connected thereto, said further membrane being composed of a further water soluble photosensitive resin including polyvinyl pyrrolidone having a high molecular weight crosslinked to 2, 5-bis (4'"'"'-azide-2'"'"'-sulfobenzal) cyclopentanone sodium salt, and a further enzyme.