Optical method and apparatus for detecting and measuring aging, photoaging, dermal disease and pigmentation in skin

• US 4,894,547 A
• Filed: 09/28/1987
• Issued: 01/16/1990
• Est. Priority Date: 09/28/1987
• Status: Expired due to Fees

• Current Assignee: Yale University
• Original Assignee: Yale University
• Inventors: Leffell, David J., Deckelbaum, Lawrence
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US07/101,944
• Time in Patent Office: 841 Days
• Field of Search: 250/461.2, 250/461.1, 250/458.1, 250/459.1, 128/633, 128/634
• US Class Current: 250/461.2
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/444 Evaluating skin marks, e.g. mole, nevi, tumour, scar
• CPC Class Codes: A61B 5/445 Evaluating skin irritation or skin trauma, e.g. rash, eczema, wound, bed sore

Abstract

A method and apparatus for inducing fluorescence in human skin, in vivo, and for evaluating certain skin characteristics from the spectral intensity of induced fluorescence. The energy of a helium-cadmium laser is directed to a skin area to be evaluated by a fiberoptic element. The induced fluorescent energy having a wavelength band of interest above that of the laser wavelength, is directed to a device by a fiberoptic path for performing a spectral analysis of the fluorescent intensity. A ratio of chracteristic portions of fluorescent intensity of the skin area under test is developed and used to compare with different test subjects. The developed ratio, when taken in relation to the skin of subjects of different ages, indicates a marked difference in older subjects in comparison to that of very young subjects which correlates with long-term exposure of the skin to ultraviolet radiation. The method and apparatus are useful for evaluating skin pigmentation as well as for evaluating certain solid compounds such as amino acids.

Claims

1. Apparatus for inducing naturally occurring fluorescence, in vivo, in human skin and for measuring and quantifying such induced fluorescence for diagnostic purposes comprising:

means for producing laser light energy having a predetermined wavelength and power;

means responsive to said laser light energy for directing the laser light energy to an untreated skin area to induce endogenous fluorescence;

means for receiving endogenous fluorescent radiation from the skin area induced to fluorescence by said laser light energy;

means for performing a spectral analysis of said received fluorescent radiation and for developing a numerical value representative of a predetermined portion of said spectrum, said numerical value having use as a diagnostic measure of the untreated skin area induced to endogenous fluorescence; and

means responsive to said receiving means for directing said received endogenous fluorescent radiation to said spectral analysis means.

2. The apparatus of claim 1, wherein said means for producing laser light energy is a helium-cadmium laser which emits radiation at approximately 325 nm.

3. The apparatus of claim 2, wherein said laser energy is applied at a power level of between 1 to 10 mW for a predetermined length of time.

4. The apparatus of claim 3, wherein said laser energy is applied for approximately 300 ms.

5. The apparatus for claim 1, wherein said means for directing said laser light energy is a fiberoptic element.

6. The apparatus of claim 5, wherein said fiberoptic element is a central fiber of a coaxial fiberoptic probe placed from 0 mm to approximately 3 mm from the skin surface.

7. The apparatus of claim 1, wherein said means for performing spectral analysis includes a spectrograph for spectral dispersion and an optical multichannel analyzer including a linear diode array coupled to a microchannel plate intensifier.

8. The apparatus of claim 7, wherein the spectral analysis means detects fluorescence over a wavelength range of approximately 400 nm.

9. The apparatus of claim 8, wherein said range is from 350 nm to 750 nm.

10. The apparatus of claim 7, including means for recording the frequency spectrum of the detected fluorescence and for calculating a numerical value representative of a predetermined portion of said spectrum.

11. The apparatus of claim 7, including means for displaying the frequency spectrum of the detected fluorescence.

12. A method for inducing naturally occurring fluorescence in untreated human skin, in vivo, comprising the steps of:

developing laser light energy of a predetermined ultraviolet band wavelength; and

directing said laser light energy to an area of untreated skin to be induced to endogenous fluorescence.

13. The method of claim 12, wherein said light energy is produced by a laser having a wavelength of approximately 325 nm.

14. The method of claim 13, wherein said laser energy has a power of from 1 to 10 mW and is applied for a predetermined length of time.

15. The method of claim 12, wherein said directing step includes directing said ultraviolet light energy to the skin area by a fiberoptic path.

16. The method of claim 15, wherein the fiberoptic path includes a distal end, the method including arranging the distal end to be adjacent the skin area to be exposed at a distance of from 0 mm to approximately 3 mm.

17. A method for quantifying and evaluating naturally occurring induced fluorescence in human skin, in vivo, comprising the steps of:

including an untreated subject area to endogenous fluorescence by application of laser energy;

sensing spectra of the induced endogenous fluorescent radiation;

obtaining a measure of intensity of said induced endogenous fluorescent radiation spectra over a predetermined frequency band; and

using said measure of intensity as a basis to make comparisons between different test subject areas for diagnostic purposes.

18. The method of claim 17, wherein said inducing step includes exposing said skin to laser energy having a wavelength of approximately 325 nm.

19. The method of claim 18, wherein said exposing step includes exposing said skin to laser energy at a level of from 1 to 10 mW at a distance of 0 to approximately 3 mm.

20. The method of claim 18, wherein said frequency band wavelength of said measure of intensity is from 350 to 750 nm.

21. The method of claim 17, wherein said using step includes the step of employing a pattern of measured intensities over an area of skin.

22. The method of claim 21, wherein said pattern recognition step includes forming a ratio of the measured intensity at two preselected wavelengths.

23. The method of claim 22, wherein said two wavelengths are approximately 390 nm and 429 nm.

24. The method of claim 22 including the step of employing said ratio to determine the degree of long term exposure of the skin to solar ultraviolet radiation.

25. The method of claim 24, wherein different test subject areas of said using step includes solar-exposed skin and non-solar exposed skin of the same subject.

26. The method of claim 17, wherein said subject area has a particular pigment, wherein said using step including using said measure of intensity to determine the degree of pigmentation of the subject area.

27. The method of claim 26, wherein the measure of intensity is inversely proportional to pigmentation.

28. The method of claim 17 wherein said method includes the step of evaluating changes in skin pigmentation by said measure of intensity.

29. A method for inducing naturally occurring fluorescence, in vivo, in human skin and for measuring and quantifying such induced fluorescence for diagnostic purposes comprising the steps of:

developing laser light energy having a predetermined wavelength and power;

directing said laser light energy to an untreated area of skin of an individual to induce endogenous fluorescence;

sending spectra of endogenous fluorescent radiation induced from said skin area;

obtaining a measure of intensity of said induced radiation spectra over a predetermined frequency band; and

using said measure of intensity as a basis to make comparisons for diagnostic purposes between said skin area and other skin areas of the same individual.