Integral multilayer analytical element

• US 4,895,704 A
• Filed: 10/27/1987
• Issued: 01/23/1990
• Est. Priority Date: 04/23/1985
• Status: Expired due to Term

• Current Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Igarashi, Takeshi, Arai, Fuminori
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): WARDEN, JILL ALICE
• Application Number:
  US07/117,355
• Time in Patent Office: 819 Days
• Field of Search: 422/57, 422/56, 436/169, 436/170, 435/805
• US Class Current: 422/428
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: Y10S 435/805 Test papers

Abstract

An integral multilayer analytical element which comprises a water-impermeable light-transmissive support, a hydrophilic layer containing a water-absorptive hydrophilic polymer binder and a spreading layer superposed in this order, and contains at least one reagent capable of reacting with a component of a sample to produce a detectable species capable of detecting by light, which is characterized in that light-scattering particulates in an amount to make light transmittance to 10 to 2.5% are contained in the above hydrophilic layer and/or a layer located on the side of the support therefrom. This element may decrease the influence of a conventional reagent layer up to the extent being negligible without lowering the sensitivity. Precision of the measurement at a low concentration range is also high, and reproducibility of the meaurement is well. Manufacture of this element is easy.

Claims

1. In an integral multilayer analytical element which comprises a water-impermeable light-transmissive support, a hydrophilic layer containing a water-absorptive hydrophilic polymer binder and a spreading layer superposed in this order, and containing at least one reagent capable of reacting with a component of a sample to produce a detectable species capable of detection by light, the improvement which comprises said hydrophilic layer containing light-scattering particulates in an amount to make light transmittance in the range from 10 to 2.5%.

3. The integral multilayer analytical element of claim 1, wherein said reagent is contained in said spreading layer.

4. The integral multilayer analytical element of claim 1 or 2, wherein mean particle size of said light-scattering particulates is in the range of 0.1 μ

m to 2 μ

m.

5. The integral multilayer analytical element of claim 4, wherein said light-scattering particulates are titanium dioxide particulates or barium sulfate particulates.

6. The integral multilayer analytical element of claim 1 or 2, wherein said light-scattering particulates are contained in said hydrophilic layer.

7. The integral multilayer analytical element of claim 4 or 6, wherein said reagent is contained in said hydrophilic layer.

8. The integral multilayer analytical element of claim 7 wherein a mordant is contained in said hydrophilic layer.

9. The integral multilayer analytical element of claim 1 or 2, wherein said support is selected from the group consisting of polyethylene terephthalate, polycarbonate, a cellulose ester and polystyrene.

10. The integral multilayer analytical element of claim 1 or 2, wherein said hydrophilic polymer binder is selected from the group consisting of gelatin, gelatin derivatives, agarose, pullulan, pullulan derivatives, polyacrylamide, polyvinyl alcohol and polyvinyl pyrrolidone.

11. The integral multilayer analytical element of claim 1 or 2, wherein said spreading layer is selected from the group consisting of a membrane filter, a continuous microspaces-containing porous layer, a spreading layer of woven fabric, a spreading layer of knitted fabric and a spreading layer comprising a paper containing a fibrous pulp of an organic polymer.

12. The integral multilayer analytical element of claim 1 or 2, wherein said hydrophilic layer contains a water-absorption layer.

13. The analytical element of claim 1 or 2 wherein the hydrophilic layer contains a registration layer.

2. In an integral multilayer analytical element which comprises a water-impermeable light-transmissive support, a hydrophilic layer containing a water-absorptive hydrophilic polymer binder and a spreading layer superposed in this order, and containing at least one reagent capable of reacting with a component of a sample to produce a detectable species capable of detection by light, the improvement which comprises an additional layer on the same side of the support as said hydrophilic layer, said additional layer containing light-scattering particulates in an amount to make light transmittance in the range from 10 to 2.5%.

14. In a method for the detection of a blood component wherein a blood sample is placed on an integral multilayer analytical element and analyzed the improvement wherein said analytical element comprises a superposed in this order, a water-permeable light-transmissive support, a hydrophilic layer containing a water-absorptive hydrophilic polymer binder, and a spreading layer, and which further contains at least one reagent capable of reacting with a component of the sample to produce a detectable species capable of detection by light, and wherein said hydrophilic layer contains light scattering particulates in an amount to make the light transmittance in the range from 10 to 2.5%.

15. The method of claim 14, wherein said component is cholesterol, uric acid, creatinine, enzymes, ammonia, saccharides or proteins.