Device for liquid chromatography or immobilized enzyme reaction

• US 4,895,806 A
• Filed: 06/13/1989
• Issued: 01/23/1990
• Est. Priority Date: 02/14/1987
• Status: Expired due to Term

• Current Assignee: Millipore Ireland B.V.
• Original Assignee: Millipore Ireland B.V.
• Inventors: Sanderson, James A., Le, Minh S.
• Primary Examiner(s): Jones, Larry
• Application Number:
  US07/366,312
• Time in Patent Office: 224 Days
• Field of Search: 435/288, 435/311, 435/310
• US Class Current: 435/293.1
• CPC Class Codes: B01D 15/08 Selective adsorption, e.g. chromatography
• CPC Class Codes: B01D 15/3857 Reaction chromatography
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 2030/386 Radial chromatography, i.e. with mobile phase traversing radially the stationary phase
• CPC Class Codes: G01N 2030/527 sorbent material in form of a membrane
• CPC Class Codes: G01N 30/6017 Fluid distributors

Abstract

A device for separating molecular components from liquids. A multi-layer microporous membrane assembly is located within the housing (1) with edges of each membrane within the assembly lying adjacent to a barrier. Means (5,6) are provided for substantially preventing flow between the membrane edges and the barrier, so that flow is through the membranes in series. Inlet means (2) direct incoming liquid on to a first surface of the membrane assembly, and outlet means (3) gather and direct outgoing liquid from a second surface of the membrane assembly. The membrane material of the assembly has a ligand or an immobilized enzyme bound thereto for effecting chromatographic separation or an immobilized enzyme reaction.

Claims

1. A device for the separation of molecular components from liquids containing the same, comprising a housing, a multi-layer microporous membrane assembly within the housing wherein a plurality of membrane layers are disposed in face-to-face relation with edges of each membrane within the assembly lying adjacent to a barrier, means for substantially preventing flow between the membrane edges and the barrier, inlet means for directing incoming liquid on to a first surface of the membrane assembly, and outlet means for gathering and directing outgoing liquid from a second surface of the membrane assembly, wherein the flow of liquid is successively through the plurality of membrane layers, the membrane material of the assembly having bound thereto a ligand or an immobilised enzyme.

2. A device according to claim 1 in which the membrane assembly comprises at least 50 membrane layers.

3. A device according to claim 1 in which the barrier is formed by side wall means of the housing, and the means for substantially preventing flow between the membrane edges and the side wall means comprise first resilient sealing means between a first end wall of the housing and the periphery of the first membrane of the stack, second resilient sealing means between a second end wall of the housing and the periphery of the last membrane of the stack, and a plurality of resilient sealing washers interposed between the periphery of two adjacent membranes at intervals through the stack, the sealing means and sealing washers each having a circumferential surface making contact with the side wall means.

4. A device according to claim 3 in which sealing washers are present in the stack at intervals of from 5 to 10 membranes.

5. A device according to claim 3 in which the side wall means is a circular cylindrical wall, the membranes are circular discs and the sealing means and washers are each annular.

6. A device according to claim 1 in which the edges of the membranes lie adjacent to side wall means of the housing, and the means for substantially preventing flow between the membrane edges and the side wall means comprises a sealant composition bonded to the side wall means and to the peripheral regions of the membranes forming the stack.

7. A device according to claim 1 in which the inlet means includes flow distribution means effective to distribute incoming liquid over a substantial area of the housing before the liquid makes contact with the first surface of the membrane assembly.

8. A device for the separation of molecular components from liquids containing the same, comprising a housing, a multi-layer microporous membrane assembly within the housing comprising a membrane sheet wound around a porous core to form a plurality of layers with the edges of each layer lying adjacent to a barrier, means for substantially preventing flow between the membrane edges and the barrier, inlet means for directing incoming liquid on to a first surface of the membrane assembly, and outlet means for gathering and directing outgoing liquid from a second surface of the membrane assembly, the membrane material of the assembly having bound thereto a ligand or an immobilised enzyme.

9. A device according to claim 8 in which the wound edges of the membrane sheet abut against a barrier formed by sealant composition bonded to the edge regions of the membrane sheet, the composition also forming the means for substantially preventing flow between the membrane edges and the barrier.

10. A device for the separation of molecular components from liquids containing the same, comprising a housing, a multi-layer microporous membrane assembly within the housing with edges of each membrane within the assembly lying adjacent to a barrier, means for substantially preventing flow between the membrane edges and the barrier, inlet means for directing incoming liquid on to a first surface of the membrane assembly including a flow distribution means effective to distribute incoming liquid over a substantial area of the housing before the liquid makes contact with the first surface of the membrane assembly comprising a porous disc overlying the first surface of the membrane assembly, said disc having a solid, non-porous section immediately aligned with an inlet opening, and a distributor arrangement for distributing incoming liquid over the surface of the porous disc, and outlet means for gathering and directing outgoing liquid from a second surface of the membrane assembly, the membrane material of the assembly having bound thereto a ligand or an immobilised enzyme.

11. A device according to claim 10 in which the inlet opening is formed in a cap forming an end wall of the housing, an inner surface of the cap lies in contact with the porous disc, and the distributor arrangement comprises a series of channels formed in the inner surface of the cap.

12. A device according to claim 11 in which the total void volume in the channels does not exceed 1% of the volume of the membrane assembly.

13. A device according to claim 11 in which the flow regime throughout the channel system is substantially constant with a Reynolds number not exceeding 1500 under normal operating conditions.

14. A device according to claim 11 in which the cap is circular, the inlet is at the centre of the cap, and the channels form a dendritic arrangement radiating outwardly from the centre of the cap.

15. A device according to claim 14 in which the sum of the shortest paths from any point on the inner surface of the cap to the two nearest channels does not exceed one tenth the diameter of the membrane assembly.