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Colorimetric ethanol analysis method and test device

  • US 4,900,666 A
  • Filed: 02/18/1988
  • Issued: 02/13/1990
  • Est. Priority Date: 07/12/1983
  • Status: Expired due to Fees
First Claim
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1. A dry room-temperature-stable test device for detecting ethanol in a aqueous fluid, which comprises an inert support pad containing stabilized alcohol oxidase in a dry form, wherein said stabilized alcohol oxidase comprises alcohol oxidase in intimate admixture with an effective alcohol oxidase stabilizing amount of a stabilizer comprising a solid aliphatic polyhydroxyl compound having from 5 to 8 carbon atoms and 5 to 7 hydroxyl groups, a substance having peroxidative activity, and a substance which will react with hydrogen peroxide in the presence of said substance having peroxidative activity to give a compound of changed color, wherein ethanol in said aqueous fluid reacts with the alcohol oxidase to yield hydrogen peroxide which reacts with the substance having peroxidative activity and the substance which gives the compound of changed color.

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