Fluorescence polarization immunoassy and reagents for measurement of c-reactive protein

• US 4,902,630 A
• Filed: 07/14/1988
• Issued: 02/20/1990
• Est. Priority Date: 07/22/1985
• Status: Expired due to Fees

• Current Assignee: Abbott Laboratories North Chicago IL
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Chiapetta, Enrico G., Bennett, Larry G.
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Spiegel, Jack
• Application Number:
  US07/219,618
• Time in Patent Office: 586 Days
• Field of Search: 436/501, 436/536, 436/546, 436/800, 436/537, 436/815
• US Class Current: 436/546
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: G01N 33/6893 related to diseases not provided for elsewhere
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine
• CPC Class Codes: Y10S 436/815 Test for named compound or class of compounds

Abstract

This disclosure relates to a fluorescence polarization immunoassay method for determining C-reactive protein in liquids, especially in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine. This disclosure also relates to novel reagents useful in such fluorescence polarization immunoassays.

Claims

1. A method for determining the presence of concentration of C-reactive protein in a liquid sample of biological fluid, comprising intermixing the a sample of said biological fluid a fluorescent tracer comprising a conjugate of C-reactive protein and a fluorescent moiety, so as to be specifically recognizable by a common antibody or receptor binding site with a solution of an antibody capable of specifically recognizing the C-reactive protein in the sample and the tracer;

and determining the amount of the tracer which becomes bound to the antibody by fluorescence polarization as a measure of the amount of C-reactive protein in the sample.

2. The method of claim 1 where said step (a) is conducted at a pH sufficient to maintain said fluorescent tracer in an ionized state.

3. The method of claim 1 where said fluorescent moiety is 5(4,6-dichlorotriazin-2-yl)-amino fluorescein.

4. A compound useful in a fluorescence polarization assay for determining the presence or amount of a C-reactive protein in a liquid sample of a biological fluid, which compound consisting of C-reactive protein conjugated to 5(4,6-dichlorotriazin-2-yl)amino fluorescein.