Method and apparatus for removal of cells from bone marrow

• US 4,904,391 A
• Filed: 10/09/1985
• Issued: 02/27/1990
• Est. Priority Date: 10/09/1985
• Status: Expired due to Fees

• Current Assignee: University of Florida (State University System of Florida)
• Original Assignee: University of Florida (State University System of Florida)
• Inventors: Freeman, Richard B.
• Primary Examiner(s): Jones, W. Gary
• Application Number:
  US06/785,729
• Time in Patent Office: 1,602 Days
• Field of Search: 210/87, 210/97, 210/137, 210/222, 210/223, 210/695, 210/927, 435/2, 424/3, 424/7.1
• US Class Current: 210/695
• CPC Class Codes: B03C 1/01 by addition of magnetic adjuvants
• CPC Class Codes: B03C 1/035 Open gradient magnetic separators, i.e. separators in which the gap is unobstructed, characterised by the configuration of the gap
• CPC Class Codes: C12M 47/04 Cell isolation or sorting purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 33/56972 White blood cells
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C

Abstract

An improved system for removing cells from bone marrow wherein the cells are bound by monoclonal antibodies conjugated to magnetic particles, the system comprising a chamber provided with inlets and outlets for flowing through the chamber a liquid sample containing the bone marrow and magnetic conjugated antibodies bound to the cells and a magnetic field source associated with the chamber wherein the improvement comprises a non-uniform magnetic field in an ascending gradient from the inlet to the outlet.

Claims

1. In a method for removing cells from bone marrow wherein said cells, bound to monoclonal antibodies, conjugated to magnetic particles, said method comprising flowing a liquid sample containing said bone marrow and magnetic conjugated antibodies bound to said cells through a zone provided with an inlet and an outlet associated with a magnetic field such that said liquid sample flowing there through and passes through said magnetic field whereby said bound cells and unconjugated particles are removed from said liquid sample by magnetic attraction, the improvement wherein:

said magnetic field through which said liquid sample flows is non-uniform and is an ascending gradient from said inlet to said outlet.

2. The method of claim 1 wherein said particles are microspheres.

3. The method of claim 2 wherein said microspheres contain magnetite.

4. The method of claim 2 wherein said microspheres comprise polystyrene.

5. The method of claim 1 wherein said cells are malignant.

6. In a system for removing cells from bone marrow wherein said cells are bound by monoclonal antibodies, conjugated to magnetic particles, said system comprising (1) means defining a chamber provided with inlet and outlet means adapted to enable to flow through said chamber of a liquid sample containing said bone marrow and magnetic conjugated antibodies bound to said cells and (2) at least one magnetic field source associated with said chamber such that said liquid sample flowing therethrough passes through said magnetic field whereby said bound cells are removed from said liquid sample by magnetic attraction, the improvement wherein:

said at least one magnetic field source comprises an assembly of plural magnets arranged such that said magnetic field is non-uniform and is an ascending gradient from said inlet means to said outlet means.

said improvement further comprising means for eliminating movement in said chamber of said conjugated and any unconjugated magnetic particles after removal from said liquid sample.

7. The system of claim 6 wherein said movement elimination means is positioned so as to retard the flow of said conjugated and any unconjugated magnetic particles after capture thereof by said magnetic field.

8. The system of claim 7 wherein said movement elimination means comprises a mesh element adapted to prevent the escape of said conjugated and any unconjugated particles from said magnetic field.

9. The system of claim 8 wherein said mesh element is positioned adjacent the wall or walls of said chamber to which said conjugated or any unconjugated magnetic particles are attracted by said magnetic field.