Graft copolymers of crosslinked polymers and polyoxyethylene, processes for their production, and their usage

• US 4,908,405 A
• Filed: 01/02/1986
• Issued: 03/13/1990
• Est. Priority Date: 01/04/1985
• Status: Expired due to Term

• Current Assignee: Wolfgang Rapp
• Original Assignee: Ernst Bayer
• Inventors: Bayer, Ernst, Rapp, Wolfgang
• Primary Examiner(s): Schofer, Joseph L.
• Assistant Examiner(s): Lipman, Bernard
• Application Number:
  US06/815,634
• Time in Patent Office: 1,531 Days
• Field of Search: 525/332.9, 525/61, 525/333.3, 525/333.4, 525/332.2, 525/328.8, 525/330.6
• US Class Current: 525/61
• CPC Class Codes: C08F 8/00 Chemical modification by after-treatment graft polymers, block polymers, crosslinking with unsaturated monomers or with polymers C08F251/00 - C08F299/00; of conjugated diene rubbers C08C

Abstract

The invention relates to graft copolymers of crosslinked polymers and linear polyoxyethylene, processes for their production, and their use as a substrate for peptide or nucleotide synthesis or for the covalent fixation of peptides, active protein compounds, and enzymes for affinity chromotography, diagnostic agents, and biotechnological methods.

Claims

1. A graft copolymer comprising a polymer having a degree of crosslinking of 0.05 to 10%, selected from the group consisting of a polyvinyl alcohol, a polyhydroxystyrene, a polymer produced from chloromethylated polystyrene and ethylene glycol, a polymer produced from chloromethylated polystyrene and oligoethylene glycols, a polyacrylate functionalized by hydroxy groups, and a polymethacrylate functionalized by hydroxy groups which contains polyoxyethylene chains which have an average molecular weight of 500 to 40,000;

said copolymer containing 0.02 to 15 meq free hydroxy groups per gram of copolymer, and being obtained by reacting the crosslinked polymer with ethylene oxide.

2. The graft copolymer of claim 1 wherein the amount of hydroxy groups is 0.05-10 meq/g.

3. The graft copolymer of claim 1 wherein the crosslinked polymer is crosslinked polystyrenes containing hydroxy groups in the amount of 0.05-0.7 meq/g.

4. The graft copolymer of claim 1 wherein the crosslinked polymer is polyvinyl alcohol containing hydroxy groups in the amount of 1-10 meq/g.

5. The graft copolymer of claim 1 wherein the average molecular weight of the polyoxyethylene chains is 800-10,000.

6. The graft copolymer of claim 1 wherein the average molecular weight of the polyoxyethylene chains is 2,000 to 3,000.

7. The graft copolymer of claim 1 wherein the extent of crosslinking of the copolymer is 1 to 2%.

8. The graft copolymer of claim 1 wherein the crosslinked polymer is polystyrene crosslinked with divinylbenzene.

9. The graft copolymer of claim 1 wherein the crosslinked polymer is produced from chloromethylated polystyrene and tetraethylene glycol.