Reagents for the preparation of 5'-biotinylated oligonucleotides
First Claim
1. A chemical compound of the formula
whereinBt is ##STR23## SG is an organic diradical spacer group that separates Bt from A at a distance sufficient to allow efficient complexation of Bt to avidin or streptavidin;
A is selected from the group consisting of --O--, --S--, ##STR24## wherein R and R1 are independently selected from the group consisting of H, C3-10 branched alkyl, C1-10 unbranched alkyl, C6-10 aryl, C7-12 alkaryl, and C7-12 aralkyl;
Q is selected from the group consisting of ##STR25## and salts thereof;
wherein R2 and R3 are independently selected from the group consisting of C3-10 branched alkyl and C1-12 unbranched alkyl;
R4 is selected from the group consisting of --(CRR1)m (A)n (CRR1 )m--, ##STR26## R, R1 and A are as defined above;
m=1-6;
n=0-1;
p=1-10;
q=0-10, provided that 2m+n≦
12, and further provided that 2≦
n+p+q≦
13; and
Y is any phosphate-protecting group.
3 Assignments
0 Petitions
Accused Products
Abstract
Reagents useful in the preparation of 5'"'"'-biotinylated oligonucleotides are disclosed.
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Citations
20 Claims
- 1. A chemical compound of the formula
- space="preserve" listing-type="equation">Bt-(SG)-A-Q
wherein Bt is ##STR23## SG is an organic diradical spacer group that separates Bt from A at a distance sufficient to allow efficient complexation of Bt to avidin or streptavidin; A is selected from the group consisting of --O--, --S--, ##STR24## wherein R and R1 are independently selected from the group consisting of H, C3-10 branched alkyl, C1-10 unbranched alkyl, C6-10 aryl, C7-12 alkaryl, and C7-12 aralkyl; Q is selected from the group consisting of ##STR25## and salts thereof;
wherein R2 and R3 are independently selected from the group consisting of C3-10 branched alkyl and C1-12 unbranched alkyl;
R4 is selected from the group consisting of --(CRR1)m (A)n (CRR1 )m--, ##STR26## R, R1 and A are as defined above;
m=1-6;
n=0-1;
p=1-10;
q=0-10, provided that 2m+n≦
12, and further provided that 2≦
n+p+q≦
13; and
Y is any phosphate-protecting group.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
Specification