Electrochemical sensor facilitating repeated measurement

• US 4,909,921 A
• Filed: 02/09/1989
• Issued: 03/20/1990
• Est. Priority Date: 02/10/1988
• Status: Expired due to Term

• Current Assignee: NEC Corporation
• Original Assignee: NEC Corporation
• Inventors: Ito, Narushi
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US07/308,597
• Time in Patent Office: 404 Days
• Field of Search: 357/25, 435/288, 435/817, 435/291, 204/1 E, 204/403
• US Class Current: 257/253
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/4145 specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

An electrochemical sensor wherein a FET for measuring chemical substances and a reference electrode are covered with a continuous hydrous polymer on an insulating substrate, and on the hydrous polymer, an enzyme-immobilized film is formed in a region positioned on a channel part of FET for measuring chemical substances.

Claims

1. An electrochemical sensor comprising:

a substrate having an insulative surface;

FET for measurement formed on said substrate and having a source, a drain, a channel portion and an enzyme-immobilized film disposed above said channel portion;

a reference electrode formed adjacently to said FET for measurement on said substrate; and

a hydrous polymer continuously covering said channel portion of said FET for measurement and said reference electrode.

2. An electrochemical sensor according to claim 1, wherein said hydrous polymer is constituted by a substance selected from a group consisting of albumin, agar, gelatin, alginic acid and K-carrageenan.

3. An electrochemical sensor according to claim 2, wherein said enzyme-immobilized film is formed on said hydrous polymer on said channel portion of said FET for measurement.

4. An electrochemical sensor according to claim 3, wherein a FET for comparison is further provided adjacently to said reference electrode on said substrate, said hydrous polymer being so formed as to cover said FET for comparison.

5. An electrochemical sensor according to claim 1, wherein said enzyme-immobilized film is formed on said hydrous polymer on said channel portion of said FET for measurement.

6. An electrochemical sensor having:

an insulative substrate;

a reference electrode formed on said insulative substrate;

first and second semiconductor layers formed adjacently to said reference electrode on said insulative substrate;

source and drain electrodes formed on the respective opposite sides of said first and second semiconductor layers;

an insulating film covering the respective surfaces of said first and second semiconductor layers;

a hydrous polymer covering said insulating film and said reference electrode commonly; and

an enzyme-immobilized film formed selectively on said hydrous polymer on said first semiconductor layer.

7. An electrochemical sensor according to claim 6, wherein said hydrous polymer is constituted by a substance selected from a group consisting of albumin, agar, gelatin, alginic acid and K-carrageenan.

8. An electrochemical sensor according to claim 7, wherein said first and second semiconductor layers are different in a conductivity type from each other.