Cell culture media flexible container

• US 4,910,147 A
• Filed: 09/21/1988
• Issued: 03/20/1990
• Est. Priority Date: 09/21/1988
• Status: Expired due to Term

• Current Assignee: Baxter International Inc.
• Original Assignee: Baxter International Inc.
• Inventors: Fisher, David, Gliniecki, Robert, Keilman, Michael R., Bilstad, Arnold C., Smith, Sidney T., Bacehowski, David V.
• Primary Examiner(s): Jones, Larry
• Application Number:
  US07/247,463
• Time in Patent Office: 545 Days
• Field of Search: 435/287, 435/296, 435/284, 435/286, 215/247, 215/365, 215/DIG. 3, 604/403, 604/408, 604/409, 206/484, 206/484.1, 206/484.2, 141/10
• US Class Current: 435/304.1
• CPC Class Codes: C12M 23/14 Bags
• CPC Class Codes: C12M 29/06 Nozzles; Sprayers; Spargers; Diffusers per se B01F23/231, B01J19/26

Abstract

A cell culture media container is provided. The container comprises a body constructed from flexible film and defining a containment area for containing the cell culture media, the body including a front face and a back face, the front and back face being sealed to each other along at least three sides thereof. The container includes a fill port for filling the containment area with cell culture media, the port being sealed to a face of the body and being so constructed and arranged that it extends from the face normal thereto. The container is constructed from a high barrier, optically clear, radiation sterilizable film.

Claims

1. A cell culture media container comprising:

a body constructed from flexible film that comprises an inner layer constructed from a polyethylene material, a core layer constructed from a barrier material, and an outer layer constructed from a material chosen from the group consisting of polyethylene and polyester-based material, the body defining a containment area for containing the cell culture media and including a front face and a back face, the front and back face being sealed to each other along at least three sides thereof; and

a fill port for receiving a nozzle means that fills the containment area with cell culture media, the port being sealed to a face of the body and being so constructed and arranged that it extends from the face normal thereto.

2. The cell culture media container of claim 1 wherein the film is a high barrier, optically clear, radiation sterilizable film.

3. The cell culture media container of claim 2 wherein the film is a laminate.

4. The cell culture media container of claim 1 wherein the fill port is constructed from a material that can be sonically welded.

5. The cell culture media container of claim 1 wherein the flexible film is constructed, at least in part, from a polyolefin.

6. The cell culture media container of claim 1 wherein the port is constructed from a moldable polyethylene.

7. A cell culture media container comprising:

a body, constructed from a web of film folded onto itself and sealed on at least three sides to define a containment area to be filled with a cell culture media, the web of film comprising an inner layer constructed from a polyethylene material, a core layer constructed from a barrier layer, and an outer layer constructed from a material chosen from the group consisting of polyethylene and polyester-based material, the body including, prior to being filled with the cell culture media, two faces that lie in substantially parallel planes; and

at least one port providing means for filling the containment area with cell culture media, the port being secured to a face of the body and extending outwardly therefrom normal to at least one plane defined by the faces of the body.

8. The cell culture media container of claim 7 wherein the fitment is constructed from a high density polyethylene.

9. The cell culture media container of claim 7 wherein the fitment is secured to the face of the body by having portions thereof being received within a hole punched in the web of film, and portions thereof extending outwardly from the hole.

10. The cell culture media container of claim 7 wherein the body includes at least one chevron seal for sealing a portion of the first face to a portion of the second face.

11. The cell culture media container of claim 7 wherein the barrier layer is constructed from a material chosen from the group consisting of polyvinylidene chloride and ethyl-vinyl alcohol.

12. The cell culture media container of claim 7 wherein the film includes an outer layer constructed from 5%, by weight, ethyl-vinyl acetate and 95%, by weight, polyethylene, a core layer constructed from ethyl-vinyl alcohol, and an inner layer constructed from polyethylene.

13. The cell culture media container of claim 12 wherein the film is a laminate and the layers are secured together by a polyester adhesive.

14. The cell culture media container of claim 7 including a second access port for accessing the contents of the containment area.

15. A container for housing product, the container constructed from a web of flexible film and comprising:

a top edge, a bottom edge, and side edges, each of the side edges having a greater length than a length of either of the top edge or bottom edge and the side edges including portions thereof that do not extend perpendicular to the top edge, one portion of one of the side edges extending outwardly from a remaining portion of the edge at an angle θ

that is greater than 0°

, the container including a fill port.

16. The container of claim 15 wherein the portion of the side edge that extends outwardly defines, with portions of the body, a fill segment of the container, the fill port being secured to the fill segment of the container.

17. The container of claim 16 including an access port secured to the container at a location other than the fill amount of the container.

18. The container of claim 15 wherein one portion of one of the side edges extends inwardly from remaining portions of the side edge at an angle φ

that is greater than 0° and

less than 90°

.

19. A method for storing a fluid in a flexible container comprising the steps of:

at least partially filling a flexible container constructed from a web of film with a fluid, by feeding fluid into the container through a fill port in the container;

sealing a portion of the flexible container to define two sealed portions, one of the portions including the fill port; and

severing the sealed portion of the container having the fill port from remaining portions of the container.

20. The method of claim 19 including the step of sealing the fill port before sealing the portion of the container.

21. The product of the process of claim 20.

22. The method of claim 19 including the step of contemporaneously sealing and severing a portion of the bag.

23. The product of the process of claim 19.

24. A container for housing product made in accordance with the method of claim 19, the container constructed from a web of flexible film and further including a top-edge, a bottom edge, side edges, and a fill port.