Method for forming a miniaturized biological assembly

• US 4,911,782 A
• Filed: 03/28/1988
• Issued: 03/27/1990
• Est. Priority Date: 03/28/1988
• Status: Expired due to Term

• Current Assignee: Cytonics Corporation, MD. Corp.
• Original Assignee: Cyto-Fluidics Incorporated
• Inventors: Brown, James F.
• Primary Examiner(s): Powell, William A.
• Application Number:
  US07/174,163
• Time in Patent Office: 729 Days
• Field of Search: 422/99, 422/73, 222/129, 356/36, 356/244, 156/629, 156/630, 156/633, 156/643, 156/646, 156/654, 156/655, 156/659.1, 156/668, 156/657, 156/644, 156/663
• US Class Current: 216/33
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2200/142 Preventing evaporation
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00

Abstract

A method of making a miniature chamber assembly that provides a miniature capillary environment in which a liquid medium containing microscopic size particulate material can be placed for study under a microscope includes the steps of forming components which are inadequate as to wettability relative to the liquid medium, altering the wettability of the components relative to the liquid medium so that they can provide a miniaturized capillary environment that can contain the liquid medium with particulate material for a time sufficient to prevent deterioration while being studied, and assembling the components to define the miniaturized capillary environment.

Claims

1. The method of making a miniaturized assembly to facilitate study of biological samples in a liquid medium while under magnification, which comprises the steps of:

a forming components which are inadequate as to wettablity, relative to the liquid medium, to define a miniaturized capillary environment containing the sample for a time sufficient to prevent deterioration of the sample while it is being studied;

b altering the wettability of the components relative to the liquid medium so that they may define a benign, miniaturized capillary environment containing the sample for a time sufficient to prevent deterioration of the sample while it is being studied; and

c assembling the components to define the benign, miniaturized capillary environment.

2. The method as defined in claim 1 wherein the surface energy of at least one component is altered in step b to a value of at least 100 dynes per centimeter.

3. The method as defined in claim 1 wherein the surface energy of at least one component is altered in step b to a value of not more than 30 dynes per centimeter.

4. The method as defined in claim 1 wherein the surface energy of at least one component is altered in step b to a value of at least 100 dynes per centimeter while the surface energy of another component is simultaneously altered to a value of not more than 30 dynes per centimeter.

5. The method as defined in claim 1 wherein the surface energy of at least one component is altered in step b to a value of not more than 5 dynes per centimeter.

6. The method as defined in claim 1 wherein the surface energy of at least one component is altered in step b to a value of at least 300 dynes per centimeter.

7. The method as defined in claim 1 wherein the surface energy of at least one component is altered in step b to a value of at least 300 dynes per centimeter while the surface energy of another component is simultaneously altered to a value of not more than 5 dynes per centimeter.

8. The method as defined in claim 1 including the step, before step c of forming one component to define a portion of the miniaturized, benign capillary environment having exposed, contiguous, miniaturized surface portions and simultaneously altering such surface portions during step c.

9. The method of making a miniature chamber assembly to facilitate study of microscopic size particulate material contained in a medium while under magnification which comprises the steps of:

a providing two glass plates and forming a thin film of photoresist material on a surface of at least one plate in which the film is of a thickness of 0.25-250 micrometers,

b exposing the thin film to a patterned image and removing film material from the glass plate to leave discrete portions of the film in accord with the pattern,

c altering the patterned film to render it selectively wettable by the medium, and

d superimposing the second glass plate upon the selectively wettable, patterned film to form a system of miniaturized chambers between the plates and bounded by the patterned film.

10. The method as defined in claim 9 wherein the patterned film defines an entrance passage into the interior of the pattern and including the step of adhesively joining the two glass plates in superimposed relation to form a unitary assembly into which a sample may be drawn by capillary action through the entrance passage.

11. The method as defined in claim 9 including the step of exposing the one plate and the patterned thin film thereon to fluorine plasma.