Antiviral method, agents and apparatus

• US 4,915,683 A
• Filed: 03/10/1987
• Issued: 04/10/1990
• Est. Priority Date: 11/21/1986
• Status: Expired due to Fees

• Current Assignee: Medical College of Wisconsin Incorporated
• Original Assignee: Medical College of Wisconsin Incorporated
• Inventors: Sieber, Fritz
• Primary Examiner(s): RIMELL, SAMUEL G
• Application Number:
  US07/024,150
• Time in Patent Office: 1,127 Days
• Field of Search: 435/238, 435/236, 424/3, 424/89, 514/274, 544/300, 544/319, 604/4, 604/5, 604/6, 604/416
• US Class Current: 604/5.02
• CPC Class Codes: A61K 31/505 Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
• CPC Class Codes: A61K 31/515 Barbituric acids; Derivatives thereof, e.g. sodium pentobarbital
• CPC Class Codes: A61L 2/0011 using physical methods
• CPC Class Codes: A61L 2/08 Radiation
• CPC Class Codes: A61M 1/3623 Means for actively controlling temperature of blood
• CPC Class Codes: A61M 1/3681 by irradiation
• CPC Class Codes: A61M 1/3686 by removing photoactive agents after irradiation
• CPC Class Codes: C12N 2710/16663 by chemical treatment
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00

Abstract

A method of treating a body fluid which is to be infused so as to inactivate any enveloped viruses in said fluid comprises mixing the body fluid with an effective amount of a photosensitizing agent which will bind to the viruses and/or virus infected cells and photosensitize them, and then exposing the resulting mixture to visible light to excite and inactivate the viruses. An apparatus for use in the method includes at least one container which contains an effective amount of the photosensitizing agent and which has at least one wall which is permeable to visible light. A number of photosensitizing agents which can be used in the method also are disclosed.

Claims

1. The method of treating a body fluid to inactivate viruses therein which comprises first bringing said body fluid into contact with an effective amount of a photosensitizing agent which selectively binds to enveloped viruses and enveloped virus infected cells and then exposing the combination of body fluid and agent to visible light to photosensitize and inactivate the enveloped viruses in said body fluid said photosensitizing agent having the following formula:

##STR4## in which n is 1-5, x is oxygen (0), sulfur (S), --CR₁ R₂ -- or selenium (Se);

y is O or S, M is alkali metal or other basic group;

R₁ and R₂ are the same or different alkyl groups of 1 to 8 carbon; and

, R₃, R₄, R₅ and R₆ are selected from hydrogen, lower alkyl group of 1 to 5 carbons, lower alkoxy groups of 1 to 5 carbon atoms, phenyl lower alkyls;

or R₃ and R₄, or R₄ and R₅, or R₅ and R₆ are part of an aromatic ring.

2. The method of the claim 1 in which the photosensitizing agent is merocyanine 540.

3. In a blood collection system which comprises a sterile, closed blood bag which is transparent to visible light and which has integral blood collection means, the improvement which comprises the interior of said sterile, closed bag prior to the collection of blood containing a safe and effective amount of a merocyanine dye which selectively will bind to any enveloped virus in the blood to be collected so that when the blood is collected and the bag containing the blood and dye is exposed to visible light of sufficient strength for a sufficient length of time any enveloped virus present will be photosensitized and inactivated.