Zero volume cell

• US 4,915,812 A
• Filed: 06/20/1986
• Issued: 04/10/1990
• Est. Priority Date: 06/20/1986
• Status: Expired due to Fees

• Current Assignee: Molecular Devices
• Original Assignee: Molecular Devices
• Inventors: Zuk, Robert F., Parce, John W.
• Primary Examiner(s): Tung, T.
• Application Number:
  US06/876,925
• Time in Patent Office: 1,390 Days
• Field of Search: 204/400, 204/403, 204/222, 204/415
• US Class Current: 204/403.1
• CPC Class Codes: G01N 27/327 Biochemical electrodes , e.g. electrical or mechanical details for in vitro measurements

Abstract

Methods and apparatuses are described for determining the presence of an analyte in a sample suspected of containing the analyte. The method comprises contacting a concentrate of a determinable element capable of being detected by means of a semiconductive electrode with the electrode in the presence of a relatively large volume of an assay medium. The determinable element is present in the concentrate in the amount related to the amount of analyte present in the sample. The volume of the medium in diffusive communication with the concentrate is then reduced and the determinable element is detected by means of the effect that the determinable element has on the semiconductive electrode.

Claims

1. An apparatus for measuring a material capable of affecting the potential of a semiconductive working electrode, said apparatus comprising:

an assay chamber;

a semiconductive working electrode and controlling electrode serving as walls of said chamber;

means for moving said electrodes from a distal position to a proximal confronting position;

at least one channel in communication with said chamber; and

means for connecting said electrodes to an external circuit for measuring changes in the electrical characteristics of said working electrode.

2. An apparatus according to claim 1, including a reference electrode well in communication with said chamber.

3. An apparatus according to claim 1, wherein said moving means is mechanical means.

4. An apparatus according to claim 1, wherein said moving means is pneumatic means.

5. An apparatus according to claim 1, wherein aid moving means is an elastomeric sheet.

6. An apparatus according to claim 1, wherein said working electrode is fixed and said controlling electrode is at the bottom of a reciprocating piston.

7. An apparatus according to claim 1, including irradiating means positioned to irradiate with light at least one site of said working electrode.

8. An apparatus for measuring a material capable of affecting the potential of a semiconductive working electrode, said apparatus comprising:

an assay chamber;

a semiconductive working electrode and a controlling electrode serving as confronting walls of said chamber;

piston means for moving said electrodes from a distal position to a proximal confronting position;

at least one channel in communication with said chamber; and

means for connecting said electrodes to an external circuit for measuring changes in the electrical characteristics of said working electrode.

9. An apparatus according to claim 8, including first and second valve means for controlling the ingress and egress of fluid from said chamber.

10. An apparatus according to claim 8, including irradiating means positioned to irradiate with light at least one site of said working electrode.

11. An apparatus according to claim 8, including a reference electrode well in communication with said chamber.

12. An apparatus for measuring a material capable of affecting the potential of a semiconductive working electrode, said apparatus comprising;

an assay chamber;

a semiconductive working electrode and a controlling electrode serving as walls of said chamber, wherein one of said electrodes is of an elastomeric material;

a thin separating gasket defining the remaining walls of said chamber;

at least one channel in communication with said chamber; and

means for connecting said electrodes to an external circuit for measuring changes in the electrical characteristics of said working electrode.

13. A cartridge apparatus for measuring material capable of, directly or indirectly, affecting the potential of a semiconductive working electrode, said apparatus comprising:

an assay chamber;

a feeding spool for feeding an extended carrier material through said chamber and a takeup spool for receiving spent carrier material from said assay chamber, said carrier material including a plurality of sample sites suitable for containing a reagent, either directly or indirectly, capable of affecting the potential of a semiconductive working electrode;

at least one sample chamber and/or reagent chamber prior to said assay chamber in the direction of movement of said carrier material;

a semiconductive working electrode and a controlling electrode serving as walls of said assay chamber;

means for adjusting the spatial relationship between said working and control electrodes from a distal position to a proximal confronting position;

at least one channel in communication with said chamber; and

means for connecting said electrodes to an external circuit for measuring changes in the electrical characteristics of said working electrode.

14. A cartridge apparatus according to claim 13, wherein said working electrode comprises the lower portion of a housing which encloses a reference electrode chamber in communication with said assay chamber.

15. A cartridge apparatus according to claim 14, wherein a reference electrode is disposed within said reference electrode chamber.

16. A cartridge apparatus according to claim 13, including irradiating means positioned to irradiate with light at at least one site of said working electrode.

17. A filter flow cell apparatus for measuring material capable of affecting the potential of a semi-conductive working electrode, said apparatus comprising:

an assay chamber;

a semiconductive working electrode comprising at least a portion of a wall of said assay chamber;

a piston comprising a controlling electrode as at least a portion of a wall of said chamber in confronting spatial relationship to said working electrode;

means for moving said piston from a distal opposition to a proximal confronting position;

a flexible filter membrane in a lower portion of said assay chamber;

at least one inlet channel in communication with said assay chamber for introducing fluid below said membrane and at least one channel communicating with said chamber for removing fluid above said membrane; and

means for controlling said electrodes to an external circuit for measuring changes in the electrical characteristics of said working electrode.

18. A filter flow cell apparatus according to claim 17 including membrane support means separating said membrane from said working electrode and means for holding said membrane against said support means.

19. A filter flow cell apparatus according to claim 17 including irradiating means positioned to irradiate with light at at least one site of said working electrode.

20. A wicking device apparatus for measuring a material capable of affecting the potential of a semiconductive working electrode, said apparatus comprising:

an assay chamber;

a semiconductive working electrode serving as one wall of said assay chamber;

a piston comprising a central reagent chamber having an opening at the bottom of said chamber and a controlling electrode in confronting spatial relationship to said working electrode;

means for moving said piston from a distal position to a proximal confronting position to said working electrode;

means for holding a membrane against said working electrode;

wicking means external to said piston in close spatial relationship to said working electrode for receiving fluid from said membrane;

means for connecting said electrodes to an external circuit for measuring changes in the electrical characteristics of said working electrode.

21. A wicking device apparatus according to claim 20, including irradiating means positioned to irradiate with light at at least one site of said working electrode.

22. A filter device apparatus for measuring a material capable of affecting the potential of a semiconductive working electrode, said apparatus comprising:

an assay chamber;

a semiconductive working electrode serving as at least a part of one wall of said assay chamber;

a tubular movable membrane holder having a membrane serving as the flow of said holder and fitted within said assay chamber for positioning said membrane against said working electrode;

a piston within said membrane holder comprising a controlling electrode in confronting spatial relationship to said working electrode and defining at least in part a reservoir for receiving fluid from said assay chamber;

means for moving said controlling electrode from a distal position to a proximal confronting position for pressing said membrane against said working electrode;

at least one channel in communication with said assay chamber; and

means for connecting said electrodes to an external circuit for measuring changes in the electrical characteristics of said working electrode.

23. A diaphragm assay apparatus for measuring a material capable of affecting the potential of a semiconductive working electrode, said apparatus comprising:

an assay chamber;

a diaphragm member dividing said assay chamber into a liquid compartment and a pressure compartment;

a semiconductive working electrode serving as at least a part of one wall of said pressure compartment;

a controlling electrode mounted on said diaphragm in confronting relationship to said working electrode;

pressurizing means for pressurizing said pressure compartment and moving said controlling electrode into close juxtaposition to said working electrode;

at least one channel in communication with said chamber; and

means for connecting said electrodes to an external circuit for measuring changes in the electrical characteristics of said working electrode.

24. A diaphragm assay apparatus according to claim 23, wherein said external circuit measures a change in capacitance of said working electrode.

25. A diaphragm assay apparatus according to claim 23, which further comprises means positioned to irradiate with light at at least one site of said working electrode.

26. A dipstick assay apparatus for measuring a material capable of affecting the potential of a semiconductive working electrode, said apparatus comprising:

an assay chamber;

a semiconductive working electrode serving as at least a part of one wall of said assay chamber;

at least one controlling electrode positioned in a distal position from said working electrode;

an entry way for introducing a dipstick into said assay chamber;

means for moving at least one of said controlling electrodes from a distal position to a position pressing said dipstick against said working electrode;

at least one channel in communication with said assay chamber; and

means for connecting said electrodes to an external circuit for measuring changes in the electrical characteristics of said working electrode.

27. A dipstick assay apparatus according to claim 26, including a reference electrode wall in communication with said assay chamber through said channel.

28. A dipstick assay apparatus according to claim 26, including a plurality of controlling electrodes.

29. A dipstick assay apparatus according to claim 26, including irradiating means positioned to irradiate with light at at least one site of said working electrode.