Self contained immunoassay element
First Claim
1. A self-contained element for analysis of a liquid sample containing a component to be determined which comprises:
- a housing containing a capillary tube having opposed interior top and bottom surfaces, a portion of at least one of said top and bottom surfaces having bound thereto a first reagent consisting of either said component to be determined or the binding partner of said component to be determined, said bound first reagent defining a reaction zone and interactive with said component to be determined to form a first nondiffusible species, and at least one of said top and bottom surfaces including a transparent area to permit measurement of radiation from at least a portion of said reaction zone;
a labelled second reagent, said second reagent consisting of said component to be determined or the binding partner of said component to be determined and being interactive with said first nondiffusible species or said first reagent to form a second labelled species;
means for introducing at least a portion of said sample and said labelled second reagent into said capillary tube by capillary forces;
at least one reactant reservoir containing a wash solution or a third reagent which is interactive with said second labelled species, said at least one reactant reservoir being in fluid communication with said capillary tube;
a waste reservoir in fluid communication with said capillary tube adapted to receive waste fluid from said capillary tube;
and said means for introducing said sample and said labelled second reagent, said at least one reactant reservoir and said waste reservoir being housed within said housing.
6 Assignments
0 Petitions
Accused Products
Abstract
An element for analysis of a liquid sample including a capillary containing a fixed reagent in fluid communication with reagent reservoirs and a waste liquid reservoir. A sample introducing means is provided which permits analyte in a sample to interact with the fixed reagent. A labelled reagent is provided which interacts with either the fixed reagent or the analyte in a manner which permits detection of the concentration of analyte in the sample. The capillary, reagent reservoirs, waste liquid reservoir and the sample introducing means are arranged within a common housing so that reagent waste liquid need not be removed from the element.
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Citations
24 Claims
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1. A self-contained element for analysis of a liquid sample containing a component to be determined which comprises:
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a housing containing a capillary tube having opposed interior top and bottom surfaces, a portion of at least one of said top and bottom surfaces having bound thereto a first reagent consisting of either said component to be determined or the binding partner of said component to be determined, said bound first reagent defining a reaction zone and interactive with said component to be determined to form a first nondiffusible species, and at least one of said top and bottom surfaces including a transparent area to permit measurement of radiation from at least a portion of said reaction zone; a labelled second reagent, said second reagent consisting of said component to be determined or the binding partner of said component to be determined and being interactive with said first nondiffusible species or said first reagent to form a second labelled species; means for introducing at least a portion of said sample and said labelled second reagent into said capillary tube by capillary forces; at least one reactant reservoir containing a wash solution or a third reagent which is interactive with said second labelled species, said at least one reactant reservoir being in fluid communication with said capillary tube; a waste reservoir in fluid communication with said capillary tube adapted to receive waste fluid from said capillary tube; and said means for introducing said sample and said labelled second reagent, said at least one reactant reservoir and said waste reservoir being housed within said housing. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A process for analyzing for an analyte in a liquid sample within the confines of a self-contained element housing which comprises:
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introducing a liquid sample into a porous layer within said element, said porous layer containing a labelled reagent which is interactive with said analyte; passing said analyte and said labelled reagent by capillary force into a capillary having opposed top and bottom surfaces and a first reagent bound to at least one of said top and bottom surfaces, said first reagent being interactive with said analyte and said labelled reagent to form a first nondiffusible species comprising said labelled reagent, said analyte and said first reagent; washing the interior of said capillary with a liquid composition positioned in a reservoir within said element and in fluid communication with said capillary to pass the portion of the sample noninteracted with said first reagent into a wastereservoir located within said element, and rendering said labelled reagent detectable. - View Dependent Claims (15, 16, 17, 18)
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19. A process for analyzing for an analyte in a liquid sample within the confines of a self-contained element housing which comprises:
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introducing by capillary forces a liquid sample into a capillary having opposed top and bottom surfaces and a first reagent bound to at least one of said surfaces, said first reagent being interactive with said analyte to form a first nondiffusible species comprising said analyte and said first reagent; washing said capillary with a liquid wash composition positioned in a reservoir within said element, said reservoir being in fluid communication with said capillary to pass the portion of the sample noninteracted with said first reagent into a waste reservoir located within said element; passing a labelled reagent interactive with said analyte from a reservoir within said element into said capillary and directing the liquid wash composition from said capillary into said waste reservoir; incubating said labelled reagent and said first nondiffusible species to effect interaction between said analyte and said first reagent to form a second nondiffusible species; and rendering said second nondiffusible species detectable. - View Dependent Claims (20)
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21. A process for analyzing for an analyte in a liquid sample within the confines of a self-contained element housing which comprises:
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introducing a liquid sample containing an analyte into a porous layer within said element, said porous layer containing a labelled reagent; introducing by capillary forces the labelled reagent and said analyte into a capillary having an inner surface and a first reagent bound to said inner surface, said first reagent being interactive with said analyte to form a first nondiffusible species comprising said analyte and said first reagent and a second nondiffusible species comprising said first reagent and said labelled reagent; washing said capillary with a liquid composition positioned in a reservoir within said element and in fluid communication with said capillary to pass the portion of the sample and labelled reagent noninteracted with said first reagent into a waste reservoir located with in said element and rendering said labelled reagent detectable. - View Dependent Claims (22, 23, 24)
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Specification