Radio frequency plasma deposited polymers that enhance cell growth

• US 4,919,659 A
• Filed: 12/21/1987
• Issued: 04/24/1990
• Est. Priority Date: 12/16/1985
• Status: Expired due to Fees

• Current Assignee: Board of Regents of The University of Washington (University of Washington)
• Original Assignee: Board of Regents of The University of Washington (University of Washington)
• Inventors: Chinn, Joseph A., Haque, Yasmeen, Ratner, Buddy D., Horbett, Thomas A.
• Primary Examiner(s): LUSIGNAN, MICHAEL R
• Application Number:
  US07/136,457
• Time in Patent Office: 855 Days
• Field of Search: 427/2, 427/45.1, 427/40, 427/41, 435/284, 435/285, 435/286, 435/240-243, 623/1, 623/66
• US Class Current: 427/2.25
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 33/0029 using an intermediate layer of polymer
• CPC Class Codes: A61L 33/0088 by grafting of a monomer onto the substrate
• CPC Class Codes: B05D 1/62 Plasma-deposition of organic layers plasma deposition in general C23C14/00, C23C16/00
• CPC Class Codes: C03C 17/32 with synthetic or natural resins C03C17/30 takes precedence
• CPC Class Codes: C12N 2533/50 Proteins
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: Y10S 623/921 Blood vessel

Abstract

A method for enhancing bio-material capability for use as a supporting surface for cell culture is disclosed. The method requires exposure of the bio-material to a plasma polymerizable polymer gas, which includes oxygen-containing organic molecules. The material surfaces are then subjected to an RF plasma gas discharge in the presence of the gas, causing a deposition on the exposed material surfaces of a conformal overcoating of the polymer on the material. The deposition enhances fibronectin adsorption and hence cell attachment, spreading and cell growth. Preferred polymerizable gases are acetone, methanol, and ethylene oxide.

Claims

1. A method of depositing a polymeric overcoat layer on a bio-material wherein said deposited layer has an effective amount of oxygen-containing groups and an enhanced ability to absorb a protein layer on said deposited layer when exposed to a biological fluid, and wherein cell attachment, mass cell culture, cell growth or mass tissue culture on said deposited layer is enhanced, said method comprising:

exposing a surface of said bio-material to an effective amount of a gas, said gas being plasma polymerizable and includes oxygen-containing organic molecules; and

subjecting said surface, in the presence of said gas, to a plasma gas discharge wherein said enhanced protein-adsorbing surface layer is generated and attached to said exposed surface.

2. The method of claim 1 wherein said adsorbed protein layer is rich in fibronectin.

3. The method of claim 1 wherein said gas is selected from the group consisting of acetone, methanol, ethylene oxide, glutaraldehyde and mixtures thereof.

4. The method of claim 1 wherein said surface, after having been subjected to said plasma gas discharge, is further treated by exposing said surface to a biological fluid wherein a protein layer that enhances cell attachment, mass cell culture, cell growth and mass tissue culture is adsorbed by said surface.

5. The method of claim 4 wherein said protein is fibronectin and said biological fluid includes heparin.

6. The method of claim 1 wherein said material is selected from the group consisting of polymers, ceramics, glasses and metals.

7. The method of claim 6 wherein said polymer is selected from the group consisting of polyethylene, polyesters, polyacrylics, polyurethane, polystyrene, and silicon-containing polymers.

8. The method of claim 1 wherein said plasma gas deposition is generated by radio-frequency means.

9. The method of claim 1 wherein said plasma gas deposition is generated by microwave frequency means.

10. The method of claim 1 wherein said modified material surface characteristics result from said plasma-deposited polymer forming a conformal, polymeric overcoating on said material, said modified surfaces further characterized as being polar in nature and including oxygen-containing groups pendent upon said surface.

11. A biological implant article that promotes endothelial cell growth and heparin binding, said article consisting essentially of:

a porous polymer material selected from the group consisting of polyester, tetrafluoroethylene and polyurethane; and

an overcoated surface layer covalently bound to said porous polymer material and comprising a plasma gas discharge layer of gas polymer selected from the group consisting of acetone, methanol, ethylene oxide, gluteraldehyde and mixtures thereof.

12. A composite support member for cell culture comprising a body of bio-material and a plasma-polymerized surface deposit layer thereon, said surface deposit layer consisting essentially of a plasma-polymerized deposit layer from and effective amount of a plasma-polymerizable organic gas, wherein said gas includes oxygen-containing organic molecules, and covalently bonded to the surface of said bio-material through pendent oxygen-containing groups of the plasma-polymerizable gas.

13. A support member according to claim 12 wherein said bio-material is selected from the group consisting of polymers, ceramics, glasses, and metals.

14. A support member according to claim 13 wherein said polymer is selected from the group consisting if polyethylenes, polyesters, polyacrylics, polyurethanes, polystyrenes, and silicon-containing polymers.

15. A support member according to claim 12 wherein said gas is selected from the group consisting of acetone, methanol, ethylene oxide, glutaraldehyde, and mixtures thereof.