Stimulation of hair growth

• US 4,919,664 A
• Filed: 02/19/1987
• Issued: 04/24/1990
• Est. Priority Date: 02/21/1986
• Status: Expired due to Fees

• Current Assignee: University of Dundee
• Original Assignee: University of Dundee
• Inventors: Oliver, Roy F., Jahoda, Colin A. B.
• Primary Examiner(s): Apley, Richard J.
• Assistant Examiner(s): Isabella, David J.
• Application Number:
  US07/016,224
• Time in Patent Office: 1,160 Days
• Field of Search: 623/1, 623/2, 623/11, 623/12, 623/15, 623/16, 623/8, 128/1 R, 435/240.25
• US Class Current: 128/898
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 35/36 Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells islets of Langerhans A61K35/39
• CPC Class Codes: A61K 8/985 Skin or skin outgrowth, e.g. hair, nails
• CPC Class Codes: A61Q 7/00 Preparations for affecting hair growth
• CPC Class Codes: C12N 5/0627 Hair cells

Abstract

The present invention relates to a method of stimulating hair growth in part of the skin of a mammal. The method comprises selecting lower follicular dermal cells and culturing them. An opening is then formed in the skin and the cultured dermal cells introduced through the opening in the outer epidermis layer of the skin into contact with the dermis, with said cultured dermal cells in association with epidermis cells.

The invention also provides a hair growth stimulating combination comprising cultured lower follicular dermal cells in admixture with epidermis cells, as well as such cultured dermal cells for use in the preparation of an injection composition for stimulating hair growth in human skin.

Claims

1. A method of inducing hair follicle development and hair growth of a desired hair type in part of the skin of a mammal said skin having an outer epidermis layer and an inner dermis layer, which method comprises the steps of:

selecting at least one lower follicular dermal cell to provide selected dermal cell, culturing the selected dermal cell so as to produce cultured dermal cells;

forming an opening in the outer epidermis layer of said part of the mammal; and

introducing cultured dermal cells through said opening in the epidermis layer into contact with the dermis layer in proximity to the epidermis and with at least some of said cultured dermal cells in contact with epidermal cells.

2. A method as claimed in claim 1 wherein said cultured dermal cells are subjected to aggregation by an aggregation means thereby enhancing interaction with the epidermal cells.

3. A method as claimed in claim 2 wherein said cultured dermal cells are treated with means for causing aggregation comprising an aggregation promoting glycoprotein, or glycosaminoglycan.

4. A method as claimed in claim 1 wherein said opening is in the form of a narrow incision having a depth of from 0.4 to 6 mm.

5. A method as claimed in claim 1 which method includes the step of inducing a blister defining a blister cavity in said part of the skin, and wherein said opening is made in part of the epidermis layer at said blister, and said cultured dermal cells are introduced into the blister cavity.

6. A method as claimed in claim 1 wherein a sheet of epidermis is formed into a pocket and the cultured dermal cells are enclosed inside said pocket of epidermis prior to introduction of said cultured dermal cells through said opening in the epidermis layer.

7. A method as claimed in claim 1 wherein the dermal cells are introduced in the form of a mixture with epidermal cells.

8. A method as claimed in claim 7 wherein said epidermal cells are selected from epidermal cells of inter-follicular origin and epidermal cells of intra-follicular origin.

9. A method as claimed in claim 1 wherein said mammal is additionally treated with a hair growth stimulating promoter so as to enhance growth of new hair induced by said introduced cultured dermal cells.

10. A method as claimed in claim 1 wherein said selected dermal cell comprise at least one of dermal papilla and dermal sheath cells.

11. A method as claimed in claim 1 wherein said selected dermal cells are of human scalp origin.

12. A method of treatment of the scalp skin area of a human subject, which area has sufficient hair loss and which has an outer epidermis layer and an inner dermis layer, which method comprises the steps of:

(a) selecting at least one human scalp lower follicular dermal cell;

(b) culturing at least one human scalp dermal cell so as to produce cultured dermal cells;

(c) forming an opening in the outer epidermis layer of said human scalp skin area; and

(d) introducing said cultured dermal cells through said opening in the epidermis layer into contact with the dermis layer in proximity to the epidermis and with at least some of said cultured dermal cells in contact with epidermal cells.

13. The method of treatment of claim 12 which includes the step of obtaining said at least one lower follicular dermal cell from the scalp of said human subject.

14. The method of treatment of claim 12 which includes the step of obtaining said at least one lower follicular dermal cell from the scalp of another human subject.