Detection of occult blood in feces

• US 4,920,045 A
• Filed: 09/22/1988
• Issued: 04/24/1990
• Est. Priority Date: 03/31/1987
• Status: Expired due to Term

• Current Assignee: Ikagaku Company Limited
• Original Assignee: Kyoto Medical Science Laboratory
• Inventors: Uchida, Kazuo, Okuda, Shoji
• Primary Examiner(s): Kepplinger, Esther M.
• Assistant Examiner(s): Scheiner, Laurie A.
• Application Number:
  US07/247,616
• Time in Patent Office: 579 Days
• Field of Search: 435/7, 435/268, 435/269, 435/272, 436/66, 436/175, 436/177, 436/825, 436/815
• US Class Current: 435/7.1
• CPC Class Codes: G01N 33/725 using peroxidative activity
• CPC Class Codes: Y10S 435/973 Simultaneous determination of more than one analyte
• CPC Class Codes: Y10S 436/815 Test for named compound or class of compounds
• CPC Class Codes: Y10S 436/825 Pretreatment for removal of interfering factors from sample
• CPC Class Codes: Y10T 436/25125 Digestion or removing interfering materials
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A method for detection of occult blood in a fecal sample which comprises acting a glycosidase type bacteriolytic enzyme on the fecal sample and subjecting the resulting fecal sample to simultaneous detection of hemoglobin, preferably in combination with transferrin, by an immunological measurement procedure.

Claims

1. A method for detection of occult blood in a fecal sample comprising simultaneously reacting said fecal sample with antibodies to hemoglobin and antibodies to transferrin and determining the presence of immunologic reaction products of said hemoglobin and transferrin as an indication of the presence of occult blood.

2. The method according to claim 1, wherein the fecal sample is admixed with a glycosidase capable of lysing bacterial cell walls without decomposing hemoglobin prior to the determination of hemoglobin and transferrin.

3. The method according to claim 1, wherein the fecal sample is sampled by the use of a spoon or spatula coated with anti-human hemoglobin antibody and anti-human transferrin antibody.

4. The method according to claim 2, wherein the enzyme is lysozyme.