Unilamellar lipid vesicles and method for their formation
First Claim
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1. A method of forming unilamellar lipid vesicles, comprising the steps of:
- (a) forming a solution of short-chain phospholipids having fatty acids with 6 to 8 carbon atoms and long chain phospholipids having fatty acids with at least 12 carbon atoms in an organic solvent;
(b) removing organic solvent from the solution to form a residue;
(c) dispersing the residue in an aqueous medium; and
(d) equilibrating the resulting dispersion until unilamellar lipid vesicles have formed.
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Abstract
This invention relates to unilamellar lipid vesicles comprised of short-chain phospholipids and long-chain phospholipids and a method for making the vesicles. The short-chain phospholipids are comprised of fatty acids with fewer than 9 carbons atoms and the long-chain phospholipids are comprised of fatty acids with at least 12 carbon atoms.
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Citations
37 Claims
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1. A method of forming unilamellar lipid vesicles, comprising the steps of:
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(a) forming a solution of short-chain phospholipids having fatty acids with 6 to 8 carbon atoms and long chain phospholipids having fatty acids with at least 12 carbon atoms in an organic solvent; (b) removing organic solvent from the solution to form a residue; (c) dispersing the residue in an aqueous medium; and (d) equilibrating the resulting dispersion until unilamellar lipid vesicles have formed. - View Dependent Claims (2, 3, 4)
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5. A method of forming unilamellar lipid vesicles, comprising the steps of:
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(a) forming a film of short chain phospholipids having fatty acids with 6 to 8 carbon atoms and of long-chain phospholipids having fatty acids with at least 12 carbon atoms; (b) dispersing the film in an aqueous medium; and (c) equilibrating the resulting dispersion under conditions suitable for unilamellar lipid vesicles to form. - View Dependent Claims (6)
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7. A method of forming unilamellar lipid vesicles, comprising the steps of:
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(a) forming a film of short-chain phospholipids having fatty acids with 6 to 8 carbon atoms;
long-chain phospholipids having fatty acids with at least 12 carbon atoms and at least one additional component selected from the group consisting of;
cholesterol, stearylamine, phosphatidic acid;
cardiolipin and lysophosphatidylcholine;(b) dispersing the film in an aqueous medium; and (c) equilibrating the resulting dispersion under conditions suitable for unilamellar lipid vesicles to form. - View Dependent Claims (8)
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9. A method of forming unilamellar lipid vesicles, comprising the steps of:
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(a) dissolving short-chain phospholipids having fatty acids with fewer than 9 carbon atoms and long-chain phospholipids having fatty acids with at least 12 carbon atoms in an organic solvent to form a solution thereof; (b) evaporating the organic solvent under nitrogen to form a film of short-chain phospholipids and long-chain phospholipids; (c) dispersing the film in an aqueous medium; and (d) equilibrating the resulting dispersion until unilamellar lipid vesicles have formed. - View Dependent Claims (10, 11, 12, 13)
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14. A method of forming unilamellar lipid vesicles, comprising the steps of:
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(a) forming a solution of short-chain phospholipids having fatty acids with fewer than 9 carbon atoms in organic solvent; (b) removing organic solvent to produce a first residue; (c) dispersing the first residue in an aqueous medium; (d) forming a solution of long-chain phospholipids having fatty acids with at least 12 carbon atoms in an organic solvent; (e) removing organic solvent to produce a second residue; (f) dispersing the second residue in an aqueous medium; (g) combining the dispersion of the first residue and the dispersion of the second residue; and (h) equilibrating the resulting dispersion until unilamellar lipid vesicles have formed. - View Dependent Claims (15, 16, 17, 18)
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19. A method of forming unilamellar lipid vesicles, comprising the steps of:
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(a) forming a film of short-chain phospholipids having fatty acids with fewer than 9 carbon atoms; (b) dispersing the film of short-chain phospholipids in an aqueous medium; (c) forming a film of long-chain phospholipids having fatty acids with at least 12 carbon atoms; (d) dispersing the film of long-chain phospholipids in an aqueous medium; (e) combining the dispersion of short-chain phospholipids and the dispersion of long-chain phospholipids; and (f) equilibrating the resulting dispersion under conditions suitable for unilamellar lipid vesicles to form. - View Dependent Claims (20, 21)
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22. A method of forming unilamellar lipid vesicles, comprising the steps of:
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(a) forming a film of short-chain phospholipids having fatty acids with fewer than 9 carbon atoms; (b) dispersing the film of short-chain phospholipids in an aqueous medium to form a first dispersion; (c) forming a film of long-chain phospholipids having fatty acids with at least 12 carbon atoms and at least one additional component selected from the group consisting of;
cholesterol, stearylamine, phosphatidic acid, cardiolipin and lysophosphatidylcholine;(d) dispersing the film of long-chain phospholipids and at least one additional component in an aqueous medium to form a second dispersion; (e) combining the first dispersion and the second dispersion; and (f) equilibrating the resulting dispersion under conditions suitable for unilamellar lipid vesicles to form. - View Dependent Claims (23, 24)
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25. A method of forming unilamellar lipid vesicles, comprising the steps of:
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(a) forming a solution of short-chain phospholipids having fatty acids with fewer than 9 carbon atoms in an organic solvent; (b) evaporating the organic solvent under nitrogen to form a film of short-chain phospholipids; (c) dispersing the film of short-chain phospholipids in an aqueous medium; (d) forming a solution of long-chain phospholipids having fatty acids with at least 12 carbon atoms in an organic solvent; (e) evaporating the organic solvent under nitrogen to form a film of long-chain phospholipids; (f) dispersing the film of long-chain phospholipids in an aqueous medium; (g) combining the dispersion of short-chain phospholipids and the dispersion of long-chain phospholipids; and (h) equilibrating the resulting dispersion until unilamellar lipid vesicles have formed. - View Dependent Claims (26, 27)
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28. A method of forming unilamellar lipid vesicles, comprising the steps of:
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(a) forming a solution of short-chain phospholipids having fatty acids with fewer than 9 carbon atoms in an organic solvent; (b) evaporating the organic solvent under nitrogen to form a first film; (c) dispersing the first film in an aqueous medium; (d) forming a solution of long-chain phospholipids having fatty acids with at least 12 carbon atoms and at least one additional component selected from the group consisting of cholesterol, stearylamine, phosphatidic acid, cardiolipin and lysophosphatidylcholine in an organic solvent; (e) evaporating the organic solvent under nitrogen to form a second film; (f) dispersing the second film in an aqueous medium; (g) combining the dispersion of the first film and the dispersion of the second film; and (h) equilibrating the resulting dispersion until unilamellar lipid vesicles have formed. - View Dependent Claims (29, 30)
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- 31. A unilamellar lipid vesicle comprised of short-chain phospholipids having fatty acids with fewer than 9 carbon atoms and long-chain phospholipids having fatty acids with at least 12 carbon atoms.
- 33. A composition for the delivery of a substance comprising a unilamellar lipid vesicle of short-chain phospholipids comprised of fatty acids having 6-8 carbon atoms and long-chain phospholipids comprised of fatty acids having at least 12 carbon atoms, said unilamellar lipid vesicle encapsulating the substance.
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35. In a method of forming unilamellar lipid vesicles, the improvement comprising combining a dispersion of short-chain phospholipids having fatty acids with fewer than 9 carbon atoms in a first aqueous medium and a dispersion of long-chain phospholipids having fatty acids with at least 12 carbon atoms in a second aqueous medium and equilibrating the resulting dispersion under conditions suitable for unilamellar lipid vesicles to form.
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36. A unilamellar lipid vesicle comprised of short-chain phospholipids comprised of fatty acids having 6-8 carbon atoms, long-chain phospholipids comprised of fatty acids having at least 12 carbon atoms, and at least one additional component selected from the group consisting of:
- cholesterol, stearylamine, phosphatidic acid, cardiolipin and lysopholsphatidylcholine.
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37. Unilamellar lipid vesicles having a single lipid bilayer which is comprised of:
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(a) short-chain phospholipids selected from the group consisting of dihexanoylphosphatidylcholine, diheptanoylphosphatidylcholine and dioctanoylphosphatidylcholine; and (b) long-chain phospholipids comprised of fatty acids having at least 12 carbon atoms.
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Specification