Polymer coated solid matrices and use in immunoassays
First Claim
1. In an immunoassay solid support carrying a capture molecule directly or through an interposing layer of a material facilitating covalent or adsorptive bonding of the capture molecule to the support, the improvement which comprises a layer of a polyethyleneimine on said support intermediate said support and said capture molecule and wherein there is a blocking agent present on the polyethyleneimine capture antibody composite comprising a mixture of ovalbumin and dithiothreitol.
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Accused Products
Abstract
Certain polyethyleneimines are used to coat solid carriers used in immunoassays to improve the sensitivity of the assay over what is obtained without the coating. Use of the invention in enzyme immunoassays yields sensitivities equal to that of radioimmunoassays. A post-coating is also used after the solid carrier has had a layer of capture molecule applied and before the assay is performed, to improve even further the sensitivity, reliability and dynamic range of the assay results.
130 Citations
22 Claims
- 1. In an immunoassay solid support carrying a capture molecule directly or through an interposing layer of a material facilitating covalent or adsorptive bonding of the capture molecule to the support, the improvement which comprises a layer of a polyethyleneimine on said support intermediate said support and said capture molecule and wherein there is a blocking agent present on the polyethyleneimine capture antibody composite comprising a mixture of ovalbumin and dithiothreitol.
- 9. In a method for producing a solid support substrate useful in performing an immunoassay wherein a capture molecule is attached to said solid support directly or through an interposing layer of a material facilitating covalent or adsorptive bonding of the capture molecule to said layer, the improvement which comprises applying a layer of a polyethyleneimine onto said support intermediate said support and said capture molecule and then treating the resulting product with a mixture comprising ovalbumin and dithiothreitol sufficient in amount to provide a blocking layer of the polyethyleneimine layer.
- 18. In a method for conducting an immunoassay wherein a solid support carrier having a capture antibody attached thereto either directly or through an interposing layer of a material facilitating covalent or adsorptive bonding of the capture antibody to the layer, and said antibody is contacted with a liquid in which the presence and/or concentration of an antigen is to be determined, and said contacted antibody is contacted with a labeled form of a binding partner of said antigen and the extent of attachment of such labeled form is measured to measure the presence and/ or concentration of said antigen, the improvement which comprises applying a layer of a polyethyleneimine to said support carrier intermediate said support and said capture antibody and then treating the resulting product with a mixture comprising ovalbumin and dithiothreitol sufficient in amount to provide a blocking layer on the polyethyleneimine layer.
Specification