Sample preparation chamber with mixer/grinder and sample aliquot isolation

• US 4,925,628 A
• Filed: 07/07/1987
• Issued: 05/15/1990
• Est. Priority Date: 12/16/1986
• Status: Expired due to Fees

• Current Assignee: Thermo Instrument Systems, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Ciba-Geigy Corporation (Novartis Ag)
• Inventors: Metzger, Andre, Grimm, Peter, Nau, Vance J., Nohl, Andre J.
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): Kummert, Lynn M.
• Application Number:
  US07/070,759
• Time in Patent Office: 1,043 Days
• Field of Search: 422/99, 422/100, 422/101, 422/102, 422/283, 422/68, 422/103, 436/54, 436/18, 436/174, 436/179, 436/180, 366/64, 366/65, 366/168, 366/270, 366/169
• US Class Current: 422/510
• CPC Class Codes: B01F 27/00 Mixers with rotary stirring devices in fixed receptacles magnetic mixers B01F33/45; Kneaders
• CPC Class Codes: B01F 33/5011 portable during use, e.g. hand-held
• CPC Class Codes: B01F 35/32005 Type of drive
• CPC Class Codes: B01F 35/3204 Motor driven, i.e. by means of an electric or IC motor
• CPC Class Codes: G01N 1/286 involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising microtomes G01N1/06; pulverising in general B02C; mixing in general B01F
• CPC Class Codes: Y10T 436/108331 Preservative, buffer, anticoagulant or diluent
• CPC Class Codes: Y10T 436/119163 with aspirator of claimed structure
• CPC Class Codes: Y10T 436/25625 Dilution
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

There is disclosed a sample preparation chamber for a system for preparing samples of various compositions for assay by liquid chromatography. The sample preparation chamber is includes a container having a threaded cap and a threaded, lightweight, translucent plastic cup. A stirrer/grinder shaft driven by a motor and connected to a propeller/grinder passes through the cap. The cup has a sloped bottom with a sump region, and a fill/empty pipe passes through the cap and has its outlet at or near the sump. A nozzle/fill pipe arrangement allows the walls to be washed down as liquid is pumped into the cup. A second fill pipe with its outlet spaced up from the bottom of the cup is also used, and a sample metering valve having an inlet in said cup is present. The sample metering valve is used to isolate a known volume of the sample from the rest of the sample for release back into the cup after the remaining sample has been pumped to waste for purposes of diluting the known volume of sample to a known concentration.

Claims

1. A sample preparation chamber for allowing samples of different compositions to be prepared for assay including a container having a bottom with at least one lowest region which is lower than all other regions in said bottom, said bottom having a configuration that tends to cause gravity to drive sample in said container to said at least one lowest region, comprising:

means insertable into said container for mixing non-homogeneous mixtures;

a fill/empty tube entering said container and having an inlet/outlet port located at said at least one lowest region; and

sample metering means coupled to said container for isolating a known volume of sample from sample in said container and for allowing the known volume of sample to be released back into said container;

wherein said container comprises a first cup having a lip defining a first opening, a second cup for receiving said first cup and having a flange defining a second opening and further having means for receiving said lip onto said flange, and a cap having an edge for compression against said lip for sealing said first opening; and

wherein said sample preparation chamber further comprises means for latching said cap against said first cup and said second cup, including a latch piece disposed adjacent said flange and said cap and having a notch for receiving said edge of said cap and said flange of said second cup, and a camming lever for compressing said cap edge against said flange and for compressing said lip of said first cup against said lip receiving means of said flange, with said camming lever rotatably carried by said latch piece and including a cam for contacting at least one of said cap edge and said flange of said second cup when said edge and said flange are positioned within said notch of said latch piece, where said cam is configured for compressing said cap edge against said flange of said second cup upon rotation of said camming lever.

2. The apparatus of claim 1, wherein said container has walls, and wherein the chamber further comprises a means for introducing liquids into said first cup having means for spraying the liquids against said walls of said first cup as the liquids are introduced into said first cup.

3. The apparatus of claim 1 further comprising a fill pipe means having an output port located in said first cup but displaced up from the bottom of said first cup by a predetermined clearance for allowing liquid to be introduced into said first cup.

4. The apparatus of claim 3 wherein said fill pipe means has a smaller diameter than said fill/empty tube.

5. An apparatus as defined in claim 1, further comprising means for aligning said latching means with said cap edge and said flange.

6. An apparatus as defined in claim 5, wherein said aligning means includes at least one pin carried on said cap and means defining at least one hole through said latch piece for receiving said at least me pin.

7. An apparatus as defined in claim 1, further comprising a piston mounted adjacent said camming lever and having an end contacting said camming lever for exerting a force against said lever, wherein the amount of force is selected for allowing said piston to be compressed when pressure in said first cup exceeds a predetermined amount, such that said pressure is vented through a gap formed between said flange and said edge, for automatically lowering said pressure to said predetermined amount.

8. The apparatus of claim 7 wherein said container has walls, and wherein the chamber further comprises means for introducing liquids into said first cup and having means for spraying the liquids against the walls of said first cup as the liquids are introduced into said first cup.

9. The apparatus of claim 8 further comprising a fill pipe means having an output port located in said first cup but displaced up from the bottom of said first cup by a predetermined clearance for allowing liquid to be introduced into said first cup.

10. The apparatus of claim 9 wherein said means for mixing includes means for grinding solid samples into smaller pieces.

11. A sample preparation chamber for allowing samples of different compositions to be prepared including a container comprising a cap and a chemically inert first cup having walls and a bottom with at least one lowest region, said bottom having a configuration that tends to cause gravity to drive sample in said container to said at least one lowest region, comprising:

means insertable into said container for mixing non-homogeneous fluids and for grinding samples into smaller pieces;

a fill/empty tube having a first diameter entering said container and having an inlet/outlet port located at said at least one lowest region;

sample metering means coupled to said container for isolating a known volume of sample from sample in said cup and for allowing the known volume of sample to be released back into said container; and

means disposed within said cup for spraying said walls of said cup with liquids;

wherein said first cup includes a lip defining a first opening and said container further comprises a second cup for receiving said first cup and having a flange defining a second opening and further having means for receiving said lip of said first cup, and wherein said cap includes an edge for compression against said lip for sealing said first opening; and

wherein said sample preparation chamber further comprises means for latching said cap against said first cup and said second cup, including a latch piece disposed adjacent said flange of said second cup and said cap and having a notch for receiving said edge of said cap and said flange, and a camming lever for compressing said cap edge against said flange and for compressing said lip of said first cup against said lip receiving means, with said camming lever rotatably carried by said latch piece and including a cam for contacting at least one of said cap edge and said flange of said second cup when said edge and said flange are positioned within said notch, where said cam is configured for compressing said cap edge against said flange of said second cup upon rotation of said camming lever.

12. The apparatus of claim 11 wherein said first cup is made of a material which is lightweight and allows light to pass therethrough.

13. The apparatus of claim 11 wherein said sample metering means comprises:

means for allowing a sample to enter a chamber of a known volume;

means for isolating said chamber of a known volume from the surrounding environment after the sample has been loaded therein;

sealing means in said means for isolating to isolate the sample in said chamber without entrapping an unknown volume of sample in the sealing means.

14. The apparatus of claim 11 wherein said sample metering means comprises:

a piston having a sample chamber formed therein as a cavity formed in a side of the piston, said cavity having a known volume;

a cylinder enclosing said piston such that the piston may slide therein, said cylinder having means defining an aperture therein through which said piston may slide so as to expose said cavity to the environment outside said cylinder;

means for forming a flush seal between said piston and said cylinder.

15. The apparatus of claim 11 wherein said sample metering means comprises:

means for pumping predetermined amounts of liquid into or out of means defining a first system port;

means for supplying pressurized gas or liquid to means defining a second system port;

a container for storing sample and diluted sample;

valve means for allowing said pumping means to pump sample out of said container into a sample chamber which may be selectively coupled to either said first or said second system port and for allowing sample in said sample chamber to be isolated from said container and both said first and second system ports and for allowing sample in said sample chamber to be placed back into said container.

16. The apparatus of claim 15 wherein control means causes said means for supplying pressurized gas or liquid to pressurize sample in said sample chamber before it is isolated in such a way as to minimize volume occupied by gas bubbles in said sample chamber.