Enzyme sensor
First Claim
1. An enzyme sensor for measuring a substrate concentration using an enzyme, comprising:
- an electrically conductive carbon having a graphite structure for generating an electric potential responsive to a hydrogen ion concentration;
an enzyme layer comprising an enzyme imparted with a cross-linked structure by a cross-linking agent coating a surface of said electrically conductive carbon having a graphite structure, said enzyme layer being capable of generating a change in said hydrogen ion concentration by promoting the chemical reaction of a substrate on which said enzyme is active; and
measuring means for measuring the electric potential of said electrically conductive carbon having a graphite structure.
1 Assignment
0 Petitions
Accused Products
Abstract
Disclosed is an enzyme sensor for measuring a predetermined substrate concentration by potentiometric or amperometric response on the basis of an enzyme. In one aspect, a solid-state enzyme sensor measures a predetermined substrate concentration by potentiometric response and includes a solid enzyme membrane-coated electrically conductive base, redox layer or ion-sensitive layer. In another aspect, a solid-state enzyme sensor measures a predetermined substrate concentration by amperometric response and includes a solid enzyme membrane-coated oxygen reduction catalyst layer. Also disclosed is an enzyme control layer for controlling the outflow and inflow of the enzyme layer.
-
Citations
36 Claims
-
1. An enzyme sensor for measuring a substrate concentration using an enzyme, comprising:
-
an electrically conductive carbon having a graphite structure for generating an electric potential responsive to a hydrogen ion concentration; an enzyme layer comprising an enzyme imparted with a cross-linked structure by a cross-linking agent coating a surface of said electrically conductive carbon having a graphite structure, said enzyme layer being capable of generating a change in said hydrogen ion concentration by promoting the chemical reaction of a substrate on which said enzyme is active; and measuring means for measuring the electric potential of said electrically conductive carbon having a graphite structure. - View Dependent Claims (2, 3, 4, 5, 6, 7)
-
-
8. An enzyme sensor for measuring a substrate concentration using enzyme, comprising:
-
an electrode generating an electric potential responsive to a hydrogen ion concentration, which electrode comprises an electrically conductive base and an electrolytically polymerized redox layer coating a surface of said electrically conductive base and having a redox reaction which is selected from a quinone-hydroquinone type redox reaction, an amine-quinoid type redox reaction and a poly(pyrrole), poly(chenilene) redox reaction and an enzyme layer comprising an enzyme and coating a surface of said electrode, said enzyme layer being capable of generating a change in said hydrogen ion concentration by promoting the chemical reaction of a substrate on which said enzyme is active. - View Dependent Claims (9, 10, 11, 12, 13, 14)
-
-
15. An enzyme sensor for measuring a substrate concentration using enzyme, comprising:
an electrode generating an electric potential responsive to a hydrogen ion concentration, which electrode comprises an electrically conductive base and an electrolytically polymerized redox layer coating a surface of said electrically conductive base wherein said redox layer is selected from a group of substances having a poly(pyrrole), poly(chenilene) oxidation-reduction function. - View Dependent Claims (16, 17, 18)
-
19. An enzyme sensor for measuring a substrate concentration on the basis of a molecular identifying function of an enzyme, comprising:
-
an electrode generating an electric potential responsive to a hydrogen ion concentration, which electrode comprises an electrically conductive base, an electrolytically polymerized redox layer coating a surface of said electrically conductive base, and a hydrogen ion-sensitive layer coating a surface of said redox layer; and an enzyme layer comprising an enzyme and coating a surface of said electrode, said enzyme layer being capable of generating a change in said hydrogen ion concentration by promoting the chemical reaction of a substrate on which said enzyme is active. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28)
-
-
29. An enzyme sensor for measuring a substrate concentration on the basis of a molecular identifying function of an enzyme, comprising:
-
an electrically conductive base; an oxygen reduction catalyst layer being electrolytically polymerized from an electrolyte solution containing a phthalocyanine or a porphyrin complex coating said electrically conductive base; and an enzyme layer containing an enzyme and electrolytically coating said oxygen reduction catalyst layer. - View Dependent Claims (30, 31, 32, 33, 34, 35, 36)
-
Specification