Method for rapid adherence of endothelial cells onto a surface and surfaces prepared thereby

• US 4,927,676 A
• Filed: 04/19/1989
• Issued: 05/22/1990
• Est. Priority Date: 07/01/1988
• Status: Expired due to Term

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Baldwin, Lillian P., Williams, Joel L., Montgomery, David B.
• Primary Examiner(s): PIANALTO, BERNARD D
• Application Number:
  US07/340,190
• Time in Patent Office: 398 Days
• Field of Search: 428/36, 428/364, 428/64, 428/423.1, 428/480, 428/523, 427/2, 427/38, 427/39, 427/230, 427/330, 427/372.2, 427/402
• US Class Current: 424/423
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062
• CPC Class Codes: A61L 33/0094 Physical treatment, e.g. plasma treatment
• CPC Class Codes: A61L 33/18 Use of ingredients of undetermined constitution or reaction products thereof
• CPC Class Codes: Y10T 428/1386 Natural or synthetic rubber or rubber-like compound containing
• CPC Class Codes: Y10T 428/2913 Rod, strand, filament or fiber
• CPC Class Codes: Y10T 428/31551 Of polyamidoester [polyurethane, polyisocyanate, polycarbamate, etc.]
• CPC Class Codes: Y10T 428/31786 Of polyester [e.g., alkyd, etc.]
• CPC Class Codes: Y10T 428/31938 Polymer of monoethylenically unsaturated hydrocarbon

Abstract

Small diameter plastic tubing is plasma-treated on the lumen wall and endothelial cells are adhered thereto to give antithrombogenic small diameter conduits useful for vascular grafts and other articles intended to come in contact with blood.

Claims

1. A method for endothelialization of a polymeric surface comprising contacting a polymeric surface with a plasma generated from a gaseous material comprising nitrogen whereby said polymeric surface is modified to contain surface amino groups, and applying to said modified surface sufficient endothelial cells to form a confluent layer of cells on said amino group-containing surface without a requirement for cell proliferation.

2. The method in accordance with claim 1 wherein said surface is selected from the group consisting of polyurethane, polystyrene, polyester, polyolefin and fluorinated polyolefin.

3. The method in accordance with claim 1 wherein said surface is relatively flat.

4. The method in accordance with claim 1 wherein said surface is curved.

5. The method in accordance with claim 1 wherein said material further comprises a second material selected from the group consisting of hydrogen, halogen, argon, neon, krypton and xenon, oxygen and a compound of oxygen.

6. The method in accordance with claim 1 wherein said plasma is generated using a measured power density level of about 0.001 to 400 watts per cubic centimeter generated over a frequency range from DC to 100 megahertz, said plasma being applied to said material for about 2 seconds to 12 hours at about 0°

to 200°

C., the pressure of said material being about 0.01 to 100 torr.

7. The method in accordance with claim 1 wherein said applying includes incubating.

8. The method in accordance with claim 7 wherein said incubating is carried out at a temperature of from about 10°

to 50°

C. for 1 to 120 minutes.

9. A method for applying cells to a surface comprising contacting a surface with a plasma generated from a gas whereby said surface is modified to contain reactive functional groups from said gas and applying to said modified surface sufficient cells to form a confluent layer of cells on said surface containing said functional groups without a requirement for cell proliferation.

10. The method of claim 9 wherein said cells are epithelial cells.

11. The method in accordance with claim 9 wherein said surface is selected from the group consisting of an inorganic surface and a polymeric surface.

12. The method in accordance with claim 9 wherein said gas is selected from the group consisting of nitrogen, oxygen, and a compound thereof.

13. A method comprising treating the lumen wall of a polymeric conduit with a plasma generated from a gas, said plasma causing bonding of a reactive functional group from said gas onto said lumen wall;

and sodding said lumen wall containing said reactive functional group with sufficient endothelial cells to form a confluent layer of cells adhered to said lumen wall without a requirement for cell proliferation.

14. The method of claim 13 wherein the lumen wall to be treated has an inside diameter of 4 mm or less.

15. The method in accordance with claim 13 wherein said gas is selected from the group consisting of oxygen, nitrogen and a compound thereof.

16. An antithrombogenic article comprising a plasma-treated polymeric surface having amino groups bonded thereto and a confluent layer of endothelial cells adhered to said surface having amino groups.

17. The article in accordance with claim 16 wherein said polymeric surface is selected from the group consisting of a polyolefin surface, a polyurethane surface, a fluorinated polyolefin surface, a polyester surface and a polystyrene surface.

18. The article in accordance with claim 16 selected from the group consisting of a plate, strip, film, sheet, fiber, fabric, conduit and cast.

19. An antithrombogenic article comprising a plasma-treated surface having reactive functional groups bonded thereto and a confluent layer of epithelial cells adhered to said surface containing reactive functional groups.

20. The article in accordance with claim 19 wherein said functional groups are selected from the group consisting of amino groups and groups containing oxygen.

21. A polymeric conduit having an inside diameter less than about 6 mm comprising a plasma-treated lumen wall, said wall having amino groups bonded thereto and a confluent layer of endothelial cells adhered to said lumen wall whereby said conduit is antithrombogenic.