Process for producing histidine

• US 4,927,758 A
• Filed: 08/25/1987
• Issued: 05/22/1990
• Est. Priority Date: 02/17/1983
• Status: Expired due to Term

• Current Assignee: Kyowa Hakko Kogyo Company Limited (Kirin Holdings Company Limited)
• Original Assignee: Kyowa Hakko Kogyo Company Limited (Kirin Holdings Company Limited)
• Inventors: Oka, Tetsuo, Mizukami, Toru, Katsumata, Ryoichi
• Primary Examiner(s): Huleatt, Jayme A.
• Application Number:
  US07/089,922
• Time in Patent Office: 1,001 Days
• Field of Search: 435/107, 435/172.3, 435/252.32, 435/320, 435/840, 435/843, 935/29, 935/23, 935/60, 935/73
• US Class Current: 435/107
• CPC Class Codes: C12N 15/52 Genes encoding for enzymes or proenzymes
• CPC Class Codes: C12P 13/04 Alpha- or beta- amino acids other amino acids C12P13/005
• CPC Class Codes: C12P 13/06 Alanine; Leucine; Isoleucine; Serine; Homoserine
• CPC Class Codes: C12P 13/10 Citrulline; Arginine; Ornithine
• CPC Class Codes: C12P 13/22 Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
• CPC Class Codes: Y10S 435/84 Brevibacterium
• CPC Class Codes: Y10S 435/843 Corynebacterium

Abstract

Disclosed is a process for producing histidine by transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA of a DNA fragment containing a gene involved in the biosynthesis of histidine and a vector DNA, culturing the transformant in a nutrient medium, accumulating histidine in the culture medium and recovering histidine therefrom.

Claims

1. A process for producing histidine, which comprises:

transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a vector containing a DNA fragment containing a gene isolated from Corynebacterium glutamicum or Escherichia coli coding for ATP phosphoribosyl transferase;

culturing the transformant in a nutrient medium;

accumulating histidine in the culture medium; and

recovering histidine therefrom.

2. The process according to claim 1, wherein the DNA fragment can confer a microorganism resistance to the group consisting of histidine and a histidine analog.

3. The process according to claim 1, wherein the DNA fragment codes for an enzyme resistant to the group consisting of histidine and a histidine analog.

4. The process according to claim 1, wherein the host microorganism is selected from the group consisting of the species Corynebacterium glutamicum, Corynebacterium herculis, Brevibacterium flavum and Brevibacterium lactofermentum.

5. The process according to claim 1, wherein the host microorganism belongs to the genus Corynebacterium or Brevibacterium and is sensitive to lysozyme.

6. The method according to claim 5, wherein the host microorganism is Corynebacterium glutamicum L-22.

7. A microorganism belonging to the genus Corynebacterium or Brevibacterium containing a vector containing a DNA fragment containing a gene isolated from Corynbacterium glutamicum or Escherichia coli coding for ATP phosphoribosyl transferase.

8. A biologically pure culture of Corynebacterium glutamicum K32, ATCC 39281, Corynebacterium herculis K33, ATCC 39282, Brevibacterium flavum K34, ATCC 39283, or Brevibacterium lactofermentum K35, ATCC 39284.

9. A biologically pure culture of Corynebacterium glutamicum K49, FERM BP-464.